Preliminary Studies Towards the Development of DNA Biosensors for Detection of Cylindrospermopsin - A Cyanobacterial Toxin
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://scielo.pt/scielo.php?script=sci_arttext&pid=S0872-19042008000100006 |
Resumo: | The suitability of polytyramine (PTy) modified electrodes for the development of DNA biosensors is analysed in this work. PTy films were synthesised potentiodynamically on Pt electrode, from acidic aqueous solution. A cylindrospermopsin coding sequence (PKSM4 probe 5-phosphate modified) was covalently bound on the polytyramine matrix using N-hydroxysuccinimide (NHS) and 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride (EDC) as coupling agents. Changes of the open circuit potential were registered as a first approach to monitorise the immobilization and hybridization processes. The modified electrodes have been characterised by cyclic voltammetry and square wave voltammetry, using methylene blue (MB), as electroactive indicator and the successful immobilization and hybridization steps are revealed by the increase of the peak currents. The hybridization has also been confirmed by measuring the fluorescence of the modified electrodes after staining with PicoGreen® DNA dye. |
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Preliminary Studies Towards the Development of DNA Biosensors for Detection of Cylindrospermopsin - A Cyanobacterial ToxincylindrospermopsinDNA biosensorPicoGreen®polytyraminemethylene blueThe suitability of polytyramine (PTy) modified electrodes for the development of DNA biosensors is analysed in this work. PTy films were synthesised potentiodynamically on Pt electrode, from acidic aqueous solution. A cylindrospermopsin coding sequence (PKSM4 probe 5-phosphate modified) was covalently bound on the polytyramine matrix using N-hydroxysuccinimide (NHS) and 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride (EDC) as coupling agents. Changes of the open circuit potential were registered as a first approach to monitorise the immobilization and hybridization processes. The modified electrodes have been characterised by cyclic voltammetry and square wave voltammetry, using methylene blue (MB), as electroactive indicator and the successful immobilization and hybridization steps are revealed by the increase of the peak currents. The hybridization has also been confirmed by measuring the fluorescence of the modified electrodes after staining with PicoGreen® DNA dye.Sociedade Portuguesa de Electroquímica2008-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articletext/htmlhttp://scielo.pt/scielo.php?script=sci_arttext&pid=S0872-19042008000100006Portugaliae Electrochimica Acta v.26 n.1 2008reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAPenghttp://scielo.pt/scielo.php?script=sci_arttext&pid=S0872-19042008000100006Valério,E.Tenreiro,A.Abrantes,L.M.info:eu-repo/semantics/openAccess2024-02-06T17:06:54Zoai:scielo:S0872-19042008000100006Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T02:20:03.686346Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Preliminary Studies Towards the Development of DNA Biosensors for Detection of Cylindrospermopsin - A Cyanobacterial Toxin |
title |
Preliminary Studies Towards the Development of DNA Biosensors for Detection of Cylindrospermopsin - A Cyanobacterial Toxin |
spellingShingle |
Preliminary Studies Towards the Development of DNA Biosensors for Detection of Cylindrospermopsin - A Cyanobacterial Toxin Valério,E. cylindrospermopsin DNA biosensor PicoGreen® polytyramine methylene blue |
title_short |
Preliminary Studies Towards the Development of DNA Biosensors for Detection of Cylindrospermopsin - A Cyanobacterial Toxin |
title_full |
Preliminary Studies Towards the Development of DNA Biosensors for Detection of Cylindrospermopsin - A Cyanobacterial Toxin |
title_fullStr |
Preliminary Studies Towards the Development of DNA Biosensors for Detection of Cylindrospermopsin - A Cyanobacterial Toxin |
title_full_unstemmed |
Preliminary Studies Towards the Development of DNA Biosensors for Detection of Cylindrospermopsin - A Cyanobacterial Toxin |
title_sort |
Preliminary Studies Towards the Development of DNA Biosensors for Detection of Cylindrospermopsin - A Cyanobacterial Toxin |
author |
Valério,E. |
author_facet |
Valério,E. Tenreiro,A. Abrantes,L.M. |
author_role |
author |
author2 |
Tenreiro,A. Abrantes,L.M. |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Valério,E. Tenreiro,A. Abrantes,L.M. |
dc.subject.por.fl_str_mv |
cylindrospermopsin DNA biosensor PicoGreen® polytyramine methylene blue |
topic |
cylindrospermopsin DNA biosensor PicoGreen® polytyramine methylene blue |
description |
The suitability of polytyramine (PTy) modified electrodes for the development of DNA biosensors is analysed in this work. PTy films were synthesised potentiodynamically on Pt electrode, from acidic aqueous solution. A cylindrospermopsin coding sequence (PKSM4 probe 5-phosphate modified) was covalently bound on the polytyramine matrix using N-hydroxysuccinimide (NHS) and 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride (EDC) as coupling agents. Changes of the open circuit potential were registered as a first approach to monitorise the immobilization and hybridization processes. The modified electrodes have been characterised by cyclic voltammetry and square wave voltammetry, using methylene blue (MB), as electroactive indicator and the successful immobilization and hybridization steps are revealed by the increase of the peak currents. The hybridization has also been confirmed by measuring the fluorescence of the modified electrodes after staining with PicoGreen® DNA dye. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://scielo.pt/scielo.php?script=sci_arttext&pid=S0872-19042008000100006 |
url |
http://scielo.pt/scielo.php?script=sci_arttext&pid=S0872-19042008000100006 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
http://scielo.pt/scielo.php?script=sci_arttext&pid=S0872-19042008000100006 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Portuguesa de Electroquímica |
publisher.none.fl_str_mv |
Sociedade Portuguesa de Electroquímica |
dc.source.none.fl_str_mv |
Portugaliae Electrochimica Acta v.26 n.1 2008 reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
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1799137289857662976 |