Construction of a flocculent brewer's yeast strain secreting Aspergillus niger β-galactosidase

Detalhes bibliográficos
Autor(a) principal: Domingues, Lucília
Data de Publicação: 2000
Outros Autores: Onnela, M., Teixeira, J. A., Lima, Nelson, Penttilä, M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/1772
Resumo: One way of improving heterologous protein production is to use high cell density systems, one of the most attractive being the flocculating yeast production system. Also, lactose is available in large amounts as a waste product from cheese production processes. The construction of flocculent and non-flocculent brewer's yeast strains secreting β-galactosidase and growing on lactose is presented. A plasmid was constructed coding for an extracellular β-galactosidase of Aspergillus niger and having, as selective marker, the yeast CUP1 gene conferring resistance to copper. This selective marker allows for the transformation of wild-type yeasts. This work represents an important step towards the study of heterologous protein secretion by flocculent cells.
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spelling Construction of a flocculent brewer's yeast strain secreting Aspergillus niger β-galactosidaseScience & TechnologyOne way of improving heterologous protein production is to use high cell density systems, one of the most attractive being the flocculating yeast production system. Also, lactose is available in large amounts as a waste product from cheese production processes. The construction of flocculent and non-flocculent brewer's yeast strains secreting β-galactosidase and growing on lactose is presented. A plasmid was constructed coding for an extracellular β-galactosidase of Aspergillus niger and having, as selective marker, the yeast CUP1 gene conferring resistance to copper. This selective marker allows for the transformation of wild-type yeasts. This work represents an important step towards the study of heterologous protein secretion by flocculent cells.Fundação para a Ciência e a Tecnologia (FCT) – PRAXIS XXI/BD/11306/97.Finland. Center For International Mobility.European Science Foundation.Springer VerlagUniversidade do MinhoDomingues, LucíliaOnnela, M.Teixeira, J. A.Lima, NelsonPenttilä, M.20002000-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/1772eng“Applied microbiology and biotechnology”. ISSN 0175-7598. 54 (2000) 97-103.0175-759810.1007/s00253000035810952011info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:33:51Zoai:repositorium.sdum.uminho.pt:1822/1772Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:29:26.498264Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Construction of a flocculent brewer's yeast strain secreting Aspergillus niger β-galactosidase
title Construction of a flocculent brewer's yeast strain secreting Aspergillus niger β-galactosidase
spellingShingle Construction of a flocculent brewer's yeast strain secreting Aspergillus niger β-galactosidase
Domingues, Lucília
Science & Technology
title_short Construction of a flocculent brewer's yeast strain secreting Aspergillus niger β-galactosidase
title_full Construction of a flocculent brewer's yeast strain secreting Aspergillus niger β-galactosidase
title_fullStr Construction of a flocculent brewer's yeast strain secreting Aspergillus niger β-galactosidase
title_full_unstemmed Construction of a flocculent brewer's yeast strain secreting Aspergillus niger β-galactosidase
title_sort Construction of a flocculent brewer's yeast strain secreting Aspergillus niger β-galactosidase
author Domingues, Lucília
author_facet Domingues, Lucília
Onnela, M.
Teixeira, J. A.
Lima, Nelson
Penttilä, M.
author_role author
author2 Onnela, M.
Teixeira, J. A.
Lima, Nelson
Penttilä, M.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Domingues, Lucília
Onnela, M.
Teixeira, J. A.
Lima, Nelson
Penttilä, M.
dc.subject.por.fl_str_mv Science & Technology
topic Science & Technology
description One way of improving heterologous protein production is to use high cell density systems, one of the most attractive being the flocculating yeast production system. Also, lactose is available in large amounts as a waste product from cheese production processes. The construction of flocculent and non-flocculent brewer's yeast strains secreting β-galactosidase and growing on lactose is presented. A plasmid was constructed coding for an extracellular β-galactosidase of Aspergillus niger and having, as selective marker, the yeast CUP1 gene conferring resistance to copper. This selective marker allows for the transformation of wild-type yeasts. This work represents an important step towards the study of heterologous protein secretion by flocculent cells.
publishDate 2000
dc.date.none.fl_str_mv 2000
2000-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/1772
url http://hdl.handle.net/1822/1772
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv “Applied microbiology and biotechnology”. ISSN 0175-7598. 54 (2000) 97-103.
0175-7598
10.1007/s002530000358
10952011
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer Verlag
publisher.none.fl_str_mv Springer Verlag
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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