Dietary exposure to imidacloprid, and sub-lethal effects in springtails

Detalhes bibliográficos
Autor(a) principal: Silva, Andreia Sofia Jorge
Data de Publicação: 2021
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/56094
Resumo: Tese de mestrado, Biologia Humana e Ambiente, 2021, Universidade de Lisboa, Faculdade de Ciências
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spelling Dietary exposure to imidacloprid, and sub-lethal effects in springtailsColêmbolosNeonicotinóidesImidaclopridaMétodo de alimentaçãoExposição concentração-respostaTeses de mestrado - 2021Departamento de Biologia AnimalTese de mestrado, Biologia Humana e Ambiente, 2021, Universidade de Lisboa, Faculdade de CiênciasNeonicotinoids are the most widely used insecticides worldwide. A large proportion ends up in the soil, where they degrade slowly, and are likely to affect non-target species in the soil community, such as Collembola (springtails). Springtails play an important role in soil ecosystems by contributing to litter decomposition and nutrient cycling. Standard toxicity tests use soil exposure to toxicants, which does not allow for detailed observation of the individuals during the experiment. These tests are developed to expose organisms through soil to certain types of contaminants, but in nature springtails can also be exposed to them through their diet. We therefore aimed to, and successfully developed, a method for dietary exposure to toxicants, allowing us to observe springtails performance during exposure. The neonicotinoid imidacloprid was applied as a test substance, and we studied two springtail species, Folsomia quadrioculata and Hypogastrura viatica, with different life histories, habitat, and ecology, to assess potential difference in sensitivity. For each species, five replicates of 20 newly hatched animals were exposed through feed treated with one of two different applications: moistening the feed with a micropipette (estimated final concentration of 129 mg/kg imidacloprid) or soaking it in a solution with 129 ug/L imidacloprid, at room temperature. Moistening the Tilia cordata bark was most accurate, giving concentrations similar to the estimated nominal ones, while soaking resulted in 38 times higher concentrations than moistened measured method. With our established method of moistening the bark, we conducted a concentration-response experiment to study how imidacloprid affects different life history traits in juvenile H. viatica using 5 sub-lethal concentrations from 0.01 to 1.2 mg/kg dry bark. Due to time constraints as a result of the COVID-19 pandemic, which prevented access to the laboratory, it was difficult to analyze some endpoints for Folsomia quadrioculata, and since it was possible to register molting and measure body size in Hypogastrura viatica we conducted the second experiment only on that species. Exposure to the soil relevant concentrations of imidacloprid caused H. viatica to reach maturity late, reduced its molting frequency, and thus reduced its body size. Since these endpoints have negative impacts on Collembola egg production, the studied neonicotinoid shown negative effects on population growth and may therefore cause population-level effects. There is still little information about these two springtails species and especially on the life history traits for springtails in general. Therefore, in the future it would be important to use methods that allow the monitoring of effects on life history traits.Rebelo, Maria Teresa Ferreira Ramos Nabais de Oliveira, 1964-Borgå, KatrineRepositório da Universidade de LisboaSilva, Andreia Sofia Jorge2023-02-01T11:44:48Z202120212021-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10451/56094TID:202934438enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T17:03:29Zoai:repositorio.ul.pt:10451/56094Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:06:39.148257Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Dietary exposure to imidacloprid, and sub-lethal effects in springtails
title Dietary exposure to imidacloprid, and sub-lethal effects in springtails
spellingShingle Dietary exposure to imidacloprid, and sub-lethal effects in springtails
Silva, Andreia Sofia Jorge
Colêmbolos
Neonicotinóides
Imidacloprida
Método de alimentação
Exposição concentração-resposta
Teses de mestrado - 2021
Departamento de Biologia Animal
title_short Dietary exposure to imidacloprid, and sub-lethal effects in springtails
title_full Dietary exposure to imidacloprid, and sub-lethal effects in springtails
title_fullStr Dietary exposure to imidacloprid, and sub-lethal effects in springtails
title_full_unstemmed Dietary exposure to imidacloprid, and sub-lethal effects in springtails
title_sort Dietary exposure to imidacloprid, and sub-lethal effects in springtails
author Silva, Andreia Sofia Jorge
author_facet Silva, Andreia Sofia Jorge
author_role author
dc.contributor.none.fl_str_mv Rebelo, Maria Teresa Ferreira Ramos Nabais de Oliveira, 1964-
Borgå, Katrine
Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Silva, Andreia Sofia Jorge
dc.subject.por.fl_str_mv Colêmbolos
Neonicotinóides
Imidacloprida
Método de alimentação
Exposição concentração-resposta
Teses de mestrado - 2021
Departamento de Biologia Animal
topic Colêmbolos
Neonicotinóides
Imidacloprida
Método de alimentação
Exposição concentração-resposta
Teses de mestrado - 2021
Departamento de Biologia Animal
description Tese de mestrado, Biologia Humana e Ambiente, 2021, Universidade de Lisboa, Faculdade de Ciências
publishDate 2021
dc.date.none.fl_str_mv 2021
2021
2021-01-01T00:00:00Z
2023-02-01T11:44:48Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/56094
TID:202934438
url http://hdl.handle.net/10451/56094
identifier_str_mv TID:202934438
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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