Ultrasound-assisted extraction of Polyphenols from maritime pine residues with deep eutectic solvents

Detalhes bibliográficos
Autor(a) principal: Duarte, Hugo
Data de Publicação: 2022
Outros Autores: Gomes, Valentim, Aliaño-González, María José, Faleiro, Maria Leonor, Romano, Anabela, Medronho, Bruno
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.1/18673
Resumo: Deep eutectic solvents represent an important alternative in the field of green solvents due to their low volatility, non-toxicity, and low synthesis cost. In the present investigation, we propose the production of enriched polyphenolic extracts from maritime pine forest residues via an ultrasound-assisted approach. A Box–Behnken experimental design with a response surface methodology was used with six variables to be optimized: solid-to-solvent ratio, water percentage, temperature and time of extraction, amplitude, and catalyst concentration. The mixture of levulinic and formic acids achieved the highest extraction yield of polyphenols from pine needle and bark biomass. In addition, the solid-to-solvent ratio was found to be the only influential variable in the extraction (p-value: 0.0000). The optimal conditions were established as: 0.1 g of sample in 10 mL of LA:FA (70:30%, v/v) with 0% water and 0 M H2SO4 heated to 30 ◦C and extracted during 40 min with an ultrasound amplitude of 80% at 37 kHz. The bioactive properties of polyphenol-enriched extracts have been proven with significant antioxidant (45.90 ± 2.10 and 66.96 ± 2.75 mg Trolox equivalents/g dw) and antimicrobial activities. The possibility to recycle and reuse the solvent was also demonstrated; levulinic acid was successfully recovered from the extracts and reused in novel extractions on pine residues. This research shows an important alternative to obtaining polyphenolenriched extracts from forest residues that are commonly discarded without any clear application, thus opening an important window toward the valorization of such residues.
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spelling Ultrasound-assisted extraction of Polyphenols from maritime pine residues with deep eutectic solventsBox–Behnken experimental designPinus pinasterBiomassDeep eutectic solventsGreen solventsAntimicrobial activityAntioxidant activityDeep eutectic solvents represent an important alternative in the field of green solvents due to their low volatility, non-toxicity, and low synthesis cost. In the present investigation, we propose the production of enriched polyphenolic extracts from maritime pine forest residues via an ultrasound-assisted approach. A Box–Behnken experimental design with a response surface methodology was used with six variables to be optimized: solid-to-solvent ratio, water percentage, temperature and time of extraction, amplitude, and catalyst concentration. The mixture of levulinic and formic acids achieved the highest extraction yield of polyphenols from pine needle and bark biomass. In addition, the solid-to-solvent ratio was found to be the only influential variable in the extraction (p-value: 0.0000). The optimal conditions were established as: 0.1 g of sample in 10 mL of LA:FA (70:30%, v/v) with 0% water and 0 M H2SO4 heated to 30 ◦C and extracted during 40 min with an ultrasound amplitude of 80% at 37 kHz. The bioactive properties of polyphenol-enriched extracts have been proven with significant antioxidant (45.90 ± 2.10 and 66.96 ± 2.75 mg Trolox equivalents/g dw) and antimicrobial activities. The possibility to recycle and reuse the solvent was also demonstrated; levulinic acid was successfully recovered from the extracts and reused in novel extractions on pine residues. This research shows an important alternative to obtaining polyphenolenriched extracts from forest residues that are commonly discarded without any clear application, thus opening an important window toward the valorization of such residues.PTDC/ASPSIL/30619/2017MDPISapientiaDuarte, HugoGomes, ValentimAliaño-González, María JoséFaleiro, Maria LeonorRomano, AnabelaMedronho, Bruno2022-12-20T10:27:37Z2022-11-222022-12-09T20:23:12Z2022-11-22T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.1/18673engFoods 11 (23): 3754 (2022)10.3390/foods112337542304-8158info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-24T10:30:54Zoai:sapientia.ualg.pt:10400.1/18673Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:08:22.054956Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Ultrasound-assisted extraction of Polyphenols from maritime pine residues with deep eutectic solvents
title Ultrasound-assisted extraction of Polyphenols from maritime pine residues with deep eutectic solvents
spellingShingle Ultrasound-assisted extraction of Polyphenols from maritime pine residues with deep eutectic solvents
Duarte, Hugo
Box–Behnken experimental design
Pinus pinaster
Biomass
Deep eutectic solvents
Green solvents
Antimicrobial activity
Antioxidant activity
title_short Ultrasound-assisted extraction of Polyphenols from maritime pine residues with deep eutectic solvents
title_full Ultrasound-assisted extraction of Polyphenols from maritime pine residues with deep eutectic solvents
title_fullStr Ultrasound-assisted extraction of Polyphenols from maritime pine residues with deep eutectic solvents
title_full_unstemmed Ultrasound-assisted extraction of Polyphenols from maritime pine residues with deep eutectic solvents
title_sort Ultrasound-assisted extraction of Polyphenols from maritime pine residues with deep eutectic solvents
author Duarte, Hugo
author_facet Duarte, Hugo
Gomes, Valentim
Aliaño-González, María José
Faleiro, Maria Leonor
Romano, Anabela
Medronho, Bruno
author_role author
author2 Gomes, Valentim
Aliaño-González, María José
Faleiro, Maria Leonor
Romano, Anabela
Medronho, Bruno
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Sapientia
dc.contributor.author.fl_str_mv Duarte, Hugo
Gomes, Valentim
Aliaño-González, María José
Faleiro, Maria Leonor
Romano, Anabela
Medronho, Bruno
dc.subject.por.fl_str_mv Box–Behnken experimental design
Pinus pinaster
Biomass
Deep eutectic solvents
Green solvents
Antimicrobial activity
Antioxidant activity
topic Box–Behnken experimental design
Pinus pinaster
Biomass
Deep eutectic solvents
Green solvents
Antimicrobial activity
Antioxidant activity
description Deep eutectic solvents represent an important alternative in the field of green solvents due to their low volatility, non-toxicity, and low synthesis cost. In the present investigation, we propose the production of enriched polyphenolic extracts from maritime pine forest residues via an ultrasound-assisted approach. A Box–Behnken experimental design with a response surface methodology was used with six variables to be optimized: solid-to-solvent ratio, water percentage, temperature and time of extraction, amplitude, and catalyst concentration. The mixture of levulinic and formic acids achieved the highest extraction yield of polyphenols from pine needle and bark biomass. In addition, the solid-to-solvent ratio was found to be the only influential variable in the extraction (p-value: 0.0000). The optimal conditions were established as: 0.1 g of sample in 10 mL of LA:FA (70:30%, v/v) with 0% water and 0 M H2SO4 heated to 30 ◦C and extracted during 40 min with an ultrasound amplitude of 80% at 37 kHz. The bioactive properties of polyphenol-enriched extracts have been proven with significant antioxidant (45.90 ± 2.10 and 66.96 ± 2.75 mg Trolox equivalents/g dw) and antimicrobial activities. The possibility to recycle and reuse the solvent was also demonstrated; levulinic acid was successfully recovered from the extracts and reused in novel extractions on pine residues. This research shows an important alternative to obtaining polyphenolenriched extracts from forest residues that are commonly discarded without any clear application, thus opening an important window toward the valorization of such residues.
publishDate 2022
dc.date.none.fl_str_mv 2022-12-20T10:27:37Z
2022-11-22
2022-12-09T20:23:12Z
2022-11-22T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.1/18673
url http://hdl.handle.net/10400.1/18673
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Foods 11 (23): 3754 (2022)
10.3390/foods11233754
2304-8158
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
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dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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