Characterization of the lipidome profile from two brown macroalgae, Sargassum muticum and Bifurcaria bifurcata and their bioactive properties

Detalhes bibliográficos
Autor(a) principal: Santos, Fábio da Fonte
Data de Publicação: 2019
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10773/30136
Resumo: Brown macroalgae elicited great interest due to their nutritional properties and biochemical composition rich in compounds with bioactive properties that can be used in pharmaceutical, nutritional and cosmetical applications. Among the lipids in algae, polar lipids such phospholipids and glycolipids have been associated to anti-inflammatory and antioxidant properties that have triggered the interest of the scientific community. However, the polar lipid composition of brown macrolagae is poorly known. Therefore, this work intended to identify the polar lipid composition of brown macroalgae Sargassum muticum and Bifurcaria bifurcata from the Portuguese coast, and to evaluate their antioxidant properties. The characterization of the polar lipid profile was performed using mass spectrometry coupled with liquid chromatography, HILICESI-MS and MS/MS. The fatty acid profile of these polar lipids was determined by CG-MS. The polar lipid profile of Sargassum muticum and Bifurcaria bifurcata revealed the presence of 176 and 141different molecular lipid species respectively, distributed between glycolipids, phospholipids and betaine lipids. Some of the identified lipid species were previously associated with bioactive properties. Fatty acid profile present in these lipid classes includes polyunsaturated fatty acids (PUFA), namely 18:3 n-3, 18:4 n-3, 20:4 n-6 and 20:5 n-3 that have nutritional value. Both lipidic extracts revealed antioxidant activity, but with better results in the case of S. muticum highlighting best antioxidant results. In a general way, the results obtained showed that both lipid extracts can be promising for pharmaceutical, biomedical and nutritional applications, contributing to the valorisation of these two macroalgae as a source of bioactive and added-value compounds.
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spelling Characterization of the lipidome profile from two brown macroalgae, Sargassum muticum and Bifurcaria bifurcata and their bioactive propertiesBrown macroalgaeSargassum muticumBifurcaria bifurcataLipidic profileBioactivityGlycolipidsPhospholipidsAntioxidant activityBrown macroalgae elicited great interest due to their nutritional properties and biochemical composition rich in compounds with bioactive properties that can be used in pharmaceutical, nutritional and cosmetical applications. Among the lipids in algae, polar lipids such phospholipids and glycolipids have been associated to anti-inflammatory and antioxidant properties that have triggered the interest of the scientific community. However, the polar lipid composition of brown macrolagae is poorly known. Therefore, this work intended to identify the polar lipid composition of brown macroalgae Sargassum muticum and Bifurcaria bifurcata from the Portuguese coast, and to evaluate their antioxidant properties. The characterization of the polar lipid profile was performed using mass spectrometry coupled with liquid chromatography, HILICESI-MS and MS/MS. The fatty acid profile of these polar lipids was determined by CG-MS. The polar lipid profile of Sargassum muticum and Bifurcaria bifurcata revealed the presence of 176 and 141different molecular lipid species respectively, distributed between glycolipids, phospholipids and betaine lipids. Some of the identified lipid species were previously associated with bioactive properties. Fatty acid profile present in these lipid classes includes polyunsaturated fatty acids (PUFA), namely 18:3 n-3, 18:4 n-3, 20:4 n-6 and 20:5 n-3 that have nutritional value. Both lipidic extracts revealed antioxidant activity, but with better results in the case of S. muticum highlighting best antioxidant results. In a general way, the results obtained showed that both lipid extracts can be promising for pharmaceutical, biomedical and nutritional applications, contributing to the valorisation of these two macroalgae as a source of bioactive and added-value compounds.As macroalgas castanhas têm vindo a despertar um grande interesse pelas suas propriedades nutricionais e pela sua composição bioquímica nomeadamente pela presença de compostos com propriedades bioativas e por isso, com potencial aplicação nas indústrias farmacêutica nutracêutica e cosmética. Entre estes, os lípidos polares tais como os fosfolípidos e glicolípidos têm sido associados a propriedades antiinflamatórias, antioxidantes, entre outras. No entanto a composição lipídica das algas castanhas é ainda pouco conhecida. Assim, este trabalho teve como objetivo identificar a composição em lípidos polares das macroalgas castanhas Sargassum muticum e Bifurcaria bifurcata presentes na costa Portuguesa e avaliar as suas propriedades antioxidantes. A identificação do perfil lipídico destas algas foi realizada usando uma abordagem lipidómica baseada em espectrometria de massa e acoplada a cromatografia líquida HILIC-ESI-MS e MS/MS. O perfil de ácidos gordos esterificados foi analisado por GC-MS. O perfil de lípidos polares do Sargassum muticum e da Bifurcaria bifurcata revelou a presença de 176 e 141espécies moleculares lipídicas diferentes, respetivamente, distribuídas entre as classes dos glicolípidos, fosfolípidos e betaínas. Entre as espécies lipídicas identificadas, algumas foram já associadas a propriedades bioativas. O perfil em ácidos gordos presente nestas classes lipídicas inclui ácidos gordos polinsaturados (PUFA), nomeadamente o 18:3 n-3, 18:4 n-3, 20:4 n-6 e o 20:5 n-3 com grande valor nutricional. Ambos os extratos lipídicos evidenciaram atividade antioxidante tendo o S. muticum evidenciado valores mais promissores. De uma forma geral, os extratos lipídicos destas duas macroalgas revelaram ser muito promissores para aplicações farmacêuticas, biomédicas e nutricionais, permitindo assim a valorização destas duas macroalgas como fonte de compostos bioativos.2019-122019-12-01T00:00:00Z2022-01-10T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10773/30136engSantos, Fábio da Fonteinfo:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-22T11:58:17Zoai:ria.ua.pt:10773/30136Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:02:19.509746Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Characterization of the lipidome profile from two brown macroalgae, Sargassum muticum and Bifurcaria bifurcata and their bioactive properties
title Characterization of the lipidome profile from two brown macroalgae, Sargassum muticum and Bifurcaria bifurcata and their bioactive properties
spellingShingle Characterization of the lipidome profile from two brown macroalgae, Sargassum muticum and Bifurcaria bifurcata and their bioactive properties
Santos, Fábio da Fonte
Brown macroalgae
Sargassum muticum
Bifurcaria bifurcata
Lipidic profile
Bioactivity
Glycolipids
Phospholipids
Antioxidant activity
title_short Characterization of the lipidome profile from two brown macroalgae, Sargassum muticum and Bifurcaria bifurcata and their bioactive properties
title_full Characterization of the lipidome profile from two brown macroalgae, Sargassum muticum and Bifurcaria bifurcata and their bioactive properties
title_fullStr Characterization of the lipidome profile from two brown macroalgae, Sargassum muticum and Bifurcaria bifurcata and their bioactive properties
title_full_unstemmed Characterization of the lipidome profile from two brown macroalgae, Sargassum muticum and Bifurcaria bifurcata and their bioactive properties
title_sort Characterization of the lipidome profile from two brown macroalgae, Sargassum muticum and Bifurcaria bifurcata and their bioactive properties
author Santos, Fábio da Fonte
author_facet Santos, Fábio da Fonte
author_role author
dc.contributor.author.fl_str_mv Santos, Fábio da Fonte
dc.subject.por.fl_str_mv Brown macroalgae
Sargassum muticum
Bifurcaria bifurcata
Lipidic profile
Bioactivity
Glycolipids
Phospholipids
Antioxidant activity
topic Brown macroalgae
Sargassum muticum
Bifurcaria bifurcata
Lipidic profile
Bioactivity
Glycolipids
Phospholipids
Antioxidant activity
description Brown macroalgae elicited great interest due to their nutritional properties and biochemical composition rich in compounds with bioactive properties that can be used in pharmaceutical, nutritional and cosmetical applications. Among the lipids in algae, polar lipids such phospholipids and glycolipids have been associated to anti-inflammatory and antioxidant properties that have triggered the interest of the scientific community. However, the polar lipid composition of brown macrolagae is poorly known. Therefore, this work intended to identify the polar lipid composition of brown macroalgae Sargassum muticum and Bifurcaria bifurcata from the Portuguese coast, and to evaluate their antioxidant properties. The characterization of the polar lipid profile was performed using mass spectrometry coupled with liquid chromatography, HILICESI-MS and MS/MS. The fatty acid profile of these polar lipids was determined by CG-MS. The polar lipid profile of Sargassum muticum and Bifurcaria bifurcata revealed the presence of 176 and 141different molecular lipid species respectively, distributed between glycolipids, phospholipids and betaine lipids. Some of the identified lipid species were previously associated with bioactive properties. Fatty acid profile present in these lipid classes includes polyunsaturated fatty acids (PUFA), namely 18:3 n-3, 18:4 n-3, 20:4 n-6 and 20:5 n-3 that have nutritional value. Both lipidic extracts revealed antioxidant activity, but with better results in the case of S. muticum highlighting best antioxidant results. In a general way, the results obtained showed that both lipid extracts can be promising for pharmaceutical, biomedical and nutritional applications, contributing to the valorisation of these two macroalgae as a source of bioactive and added-value compounds.
publishDate 2019
dc.date.none.fl_str_mv 2019-12
2019-12-01T00:00:00Z
2022-01-10T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10773/30136
url http://hdl.handle.net/10773/30136
dc.language.iso.fl_str_mv eng
language eng
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dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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