VviRafS5 is a raffinose synthase involved in cold acclimation in grapevine woody tissues

Detalhes bibliográficos
Autor(a) principal: Noronha, Henrique
Data de Publicação: 2022
Outros Autores: Silva, Angélica, Silva, Tiago, Frusciante, Sarah, Diretto, Gianfranco, Gerós, H.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://hdl.handle.net/1822/76688
Resumo: The accumulation of raffinose family oligosaccharides (RFOs) is a hallmark of plant response to different abiotic stresses, including cold. The synthesis of galactinol, by galactinol synthases (GolS), and raffinose, by raffinose synthases (RafS), are fundamental for stress-induced accumulation of RFOs, but the role of these enzymes in the cold response of grapevine (Vitis vinifera L.) woody tissues is still unclear. To address this gap in the literature, 1-year-lignified grapevine canes were incubated at 4°C for 7 and 14days and tissues were analyzed for sugar content and gene expression. Results showed that, in parallel to starch breakdown, there was an increase in soluble sugars, including sucrose, glucose, fructose, raffinose, and stachyose. Remarkably, abscisic acid (ABA) levels increased during cold acclimation, which correlated with the increased expression of the key ABA-synthesis genes VviNCED2 and VviNCED3. Expression analysis of the VviGolS and VviRafS family allowed the identification of VviRafS5 as a key player in grapevine cold response. The overexpression of VviRafS5 in Saccharomyces cerevisiae allowed the biochemical characterization of the encoded protein as a raffinose synthase with a size of ~87kDa. In grapevine cultured cells, VviRafS5 was upregulated by cold and ABA but not by heat and salt stresses. Our results suggest that ABA accumulation in woody tissues during cold acclimation upregulates VivRafS5 leading to raffinose synthesis.
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spelling VviRafS5 is a raffinose synthase involved in cold acclimation in grapevine woody tissuesVitis viniferaRaffinose biosynthesisRFOs sugarsCold acclimationWoody tissuesScience & TechnologyThe accumulation of raffinose family oligosaccharides (RFOs) is a hallmark of plant response to different abiotic stresses, including cold. The synthesis of galactinol, by galactinol synthases (GolS), and raffinose, by raffinose synthases (RafS), are fundamental for stress-induced accumulation of RFOs, but the role of these enzymes in the cold response of grapevine (Vitis vinifera L.) woody tissues is still unclear. To address this gap in the literature, 1-year-lignified grapevine canes were incubated at 4°C for 7 and 14days and tissues were analyzed for sugar content and gene expression. Results showed that, in parallel to starch breakdown, there was an increase in soluble sugars, including sucrose, glucose, fructose, raffinose, and stachyose. Remarkably, abscisic acid (ABA) levels increased during cold acclimation, which correlated with the increased expression of the key ABA-synthesis genes VviNCED2 and VviNCED3. Expression analysis of the VviGolS and VviRafS family allowed the identification of VviRafS5 as a key player in grapevine cold response. The overexpression of VviRafS5 in Saccharomyces cerevisiae allowed the biochemical characterization of the encoded protein as a raffinose synthase with a size of ~87kDa. In grapevine cultured cells, VviRafS5 was upregulated by cold and ABA but not by heat and salt stresses. Our results suggest that ABA accumulation in woody tissues during cold acclimation upregulates VivRafS5 leading to raffinose synthesis.The work was supported by National Funds by FCT— Portuguese Foundation for Science and Technology, under the strategic program UIDB/04050/2020. The work was also supported by FCT and European Funds (FEDER/POCI/COMPETE2020) through the research projects MitiVineDrought (PTDC/BIAFBT/30341/2017 and POCI 01-0145-FEDER-030341), BerryPlastid (PTDC/BIA-FBT/28165/2017 and POCI-01-0145-FEDER-028165), and AgrifoodXXI (NORTE-01-0145-FEDER-000041). AS and HN were supported by FCT grants SFRH/BD/135782/2018 and SFRH/BPD/115518/2016, respectively. This work also benefited from the networking activities within COST ACTION CA17111 INTEGRAPE and Collaborative Laboratory “CoLab ADVID Vines & Wines.”info:eu-repo/semantics/publishedVersionFrontiers Media S.A.Universidade do MinhoNoronha, HenriqueSilva, AngélicaSilva, TiagoFrusciante, SarahDiretto, GianfrancoGerós, H.2022-02-152022-02-15T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/76688engNoronha, Henrique; Silva, Angélica; Silva, Tiago; Frusciante, Sarah; Diretto, Gianfranco; Gerós, Hernâni, VviRafS5 is a raffinose synthase involved in cold acclimation in grapevine woody tissues. Frontiers in Plant Science, 12(754537), 20221664-462X10.3389/fpls.2021.754537http://www.frontiersin.org/Plant_Physiologyinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-11T07:00:11Zoai:repositorium.sdum.uminho.pt:1822/76688Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-11T07:00:11Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv VviRafS5 is a raffinose synthase involved in cold acclimation in grapevine woody tissues
title VviRafS5 is a raffinose synthase involved in cold acclimation in grapevine woody tissues
spellingShingle VviRafS5 is a raffinose synthase involved in cold acclimation in grapevine woody tissues
Noronha, Henrique
Vitis vinifera
Raffinose biosynthesis
RFOs sugars
Cold acclimation
Woody tissues
Science & Technology
title_short VviRafS5 is a raffinose synthase involved in cold acclimation in grapevine woody tissues
title_full VviRafS5 is a raffinose synthase involved in cold acclimation in grapevine woody tissues
title_fullStr VviRafS5 is a raffinose synthase involved in cold acclimation in grapevine woody tissues
title_full_unstemmed VviRafS5 is a raffinose synthase involved in cold acclimation in grapevine woody tissues
title_sort VviRafS5 is a raffinose synthase involved in cold acclimation in grapevine woody tissues
author Noronha, Henrique
author_facet Noronha, Henrique
Silva, Angélica
Silva, Tiago
Frusciante, Sarah
Diretto, Gianfranco
Gerós, H.
author_role author
author2 Silva, Angélica
Silva, Tiago
Frusciante, Sarah
Diretto, Gianfranco
Gerós, H.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Noronha, Henrique
Silva, Angélica
Silva, Tiago
Frusciante, Sarah
Diretto, Gianfranco
Gerós, H.
dc.subject.por.fl_str_mv Vitis vinifera
Raffinose biosynthesis
RFOs sugars
Cold acclimation
Woody tissues
Science & Technology
topic Vitis vinifera
Raffinose biosynthesis
RFOs sugars
Cold acclimation
Woody tissues
Science & Technology
description The accumulation of raffinose family oligosaccharides (RFOs) is a hallmark of plant response to different abiotic stresses, including cold. The synthesis of galactinol, by galactinol synthases (GolS), and raffinose, by raffinose synthases (RafS), are fundamental for stress-induced accumulation of RFOs, but the role of these enzymes in the cold response of grapevine (Vitis vinifera L.) woody tissues is still unclear. To address this gap in the literature, 1-year-lignified grapevine canes were incubated at 4°C for 7 and 14days and tissues were analyzed for sugar content and gene expression. Results showed that, in parallel to starch breakdown, there was an increase in soluble sugars, including sucrose, glucose, fructose, raffinose, and stachyose. Remarkably, abscisic acid (ABA) levels increased during cold acclimation, which correlated with the increased expression of the key ABA-synthesis genes VviNCED2 and VviNCED3. Expression analysis of the VviGolS and VviRafS family allowed the identification of VviRafS5 as a key player in grapevine cold response. The overexpression of VviRafS5 in Saccharomyces cerevisiae allowed the biochemical characterization of the encoded protein as a raffinose synthase with a size of ~87kDa. In grapevine cultured cells, VviRafS5 was upregulated by cold and ABA but not by heat and salt stresses. Our results suggest that ABA accumulation in woody tissues during cold acclimation upregulates VivRafS5 leading to raffinose synthesis.
publishDate 2022
dc.date.none.fl_str_mv 2022-02-15
2022-02-15T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/1822/76688
url https://hdl.handle.net/1822/76688
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Noronha, Henrique; Silva, Angélica; Silva, Tiago; Frusciante, Sarah; Diretto, Gianfranco; Gerós, Hernâni, VviRafS5 is a raffinose synthase involved in cold acclimation in grapevine woody tissues. Frontiers in Plant Science, 12(754537), 2022
1664-462X
10.3389/fpls.2021.754537
http://www.frontiersin.org/Plant_Physiology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media S.A.
publisher.none.fl_str_mv Frontiers Media S.A.
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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