Signal Integration in Quorum Sensing Enables Cross-Species Induction of Virulence in Pectobacterium wasabiae
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2017 |
| Outros Autores: | , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10400.7/761 |
Resumo: | This deposit is composed by the main article plus the supplementary materials of the publication. |
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Signal Integration in Quorum Sensing Enables Cross-Species Induction of Virulence in Pectobacterium wasabiaeBacterialPectobacterium wasabiaeExpR2AHL systemVirulenceN-Acyl homoserine lactoneRNA RsmBGac/RsmThis deposit is composed by the main article plus the supplementary materials of the publication.Bacterial communities can sense their neighbors, regulating group behaviors in response to cell density and environmental changes. The diversity of signaling networks in a single species has been postulated to allow custom responses to different stimuli; however, little is known about how multiple signals are integrated and the implications of this integration in different ecological contexts. In the plant pathogen Pectobacterium wasabiae (formerly Erwinia carotovora), two signaling networks-the N-acyl homoserine lactone (AHL) quorum-sensing system and the Gac/Rsm signal transduction pathway-control the expression of secreted plant cell wall-degrading enzymes, its major virulence determinants. We show that the AHL system controls the Gac/Rsm system by affecting the expression of the regulatory RNA RsmB. This regulation is mediated by ExpR2, the quorum-sensing receptor that responds to the P. wasabiae cognate AHL but also to AHLs produced by other bacterial species. As a consequence, this level of regulation allows P. wasabiae to bypass the Gac-dependent regulation of RsmB in the presence of exogenous AHLs or AHL-producing bacteria. We provide in vivo evidence that this pivotal role of RsmB in signal transduction is important for the ability of P. wasabiae to induce virulence in response to other AHL-producing bacteria in multispecies plant lesions. Our results suggest that the signaling architecture in P. wasabiae was coopted to prime the bacteria to eavesdrop on other bacteria and quickly join the efforts of other species, which are already exploiting host resources.IMPORTANCE Quorum-sensing mechanisms enable bacteria to communicate through small signal molecules and coordinate group behaviors. Often, bacteria have various quorum-sensing receptors and integrate information with other signal transduction pathways, presumably allowing them to respond to different ecological contexts. The plant pathogen Pectobacterium wasabiae has two N-acyl homoserine lactone receptors with apparently the same regulatory functions. Our work revealed that the receptor with the broadest signal specificity is also responsible for establishing the link between the main signaling pathways regulating virulence in P. wasabiae This link is essential to provide P. wasabiae with the ability to induce virulence earlier in response to higher densities of other bacterial species. We further present in vivo evidence that this novel regulatory link enables P. wasabiae to join related bacteria in the effort to degrade host tissue in multispecies plant lesions. Our work provides support for the hypothesis that interspecies interactions are among the major factors influencing the network architectures observed in bacterial quorum-sensing pathways.Fundação Para a Ciência e Tecnologia grants: (PTDC/BIA-BCM/101585/2008, SFRH/BD/33570/2008, SRFH/BD/113986/2015, PD/00133/2012); Marie Curie Intra-European grant: (PIEF-GA-2011-301365); Howard Hughes Medical Institute international early career scientist grant: (HHMI 55007436).American Society for MicrobiologyARCAValente, Rita S.Nadal-Jimenez, PolCarvalho, André F. P.Vieira, Filipe J. D.Xavier, Karina B.2017-05-29T11:10:24Z2017-05-232017-05-23T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/10400.7/761engValente RS, Nadal-Jimenez P, Carvalho AFP, Vieira FJD, Xavier KB. 2017. Signal integration in quorum sensing enables cross- species induction of virulence in Pectobacterium wasabiae. mBio 8:e00398-17. https://doi.org/10.1128/mBio.00398-17 .10.1128/mBio.00398-17info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-11-29T14:35:09Zoai:arca.igc.gulbenkian.pt:10400.7/761Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:11:59.248756Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Signal Integration in Quorum Sensing Enables Cross-Species Induction of Virulence in Pectobacterium wasabiae |
| title |
Signal Integration in Quorum Sensing Enables Cross-Species Induction of Virulence in Pectobacterium wasabiae |
| spellingShingle |
Signal Integration in Quorum Sensing Enables Cross-Species Induction of Virulence in Pectobacterium wasabiae Valente, Rita S. Bacterial Pectobacterium wasabiae ExpR2 AHL system Virulence N-Acyl homoserine lactone RNA RsmB Gac/Rsm |
| title_short |
Signal Integration in Quorum Sensing Enables Cross-Species Induction of Virulence in Pectobacterium wasabiae |
| title_full |
Signal Integration in Quorum Sensing Enables Cross-Species Induction of Virulence in Pectobacterium wasabiae |
| title_fullStr |
Signal Integration in Quorum Sensing Enables Cross-Species Induction of Virulence in Pectobacterium wasabiae |
| title_full_unstemmed |
Signal Integration in Quorum Sensing Enables Cross-Species Induction of Virulence in Pectobacterium wasabiae |
| title_sort |
Signal Integration in Quorum Sensing Enables Cross-Species Induction of Virulence in Pectobacterium wasabiae |
| author |
Valente, Rita S. |
| author_facet |
Valente, Rita S. Nadal-Jimenez, Pol Carvalho, André F. P. Vieira, Filipe J. D. Xavier, Karina B. |
| author_role |
author |
| author2 |
Nadal-Jimenez, Pol Carvalho, André F. P. Vieira, Filipe J. D. Xavier, Karina B. |
| author2_role |
author author author author |
| dc.contributor.none.fl_str_mv |
ARCA |
| dc.contributor.author.fl_str_mv |
Valente, Rita S. Nadal-Jimenez, Pol Carvalho, André F. P. Vieira, Filipe J. D. Xavier, Karina B. |
| dc.subject.por.fl_str_mv |
Bacterial Pectobacterium wasabiae ExpR2 AHL system Virulence N-Acyl homoserine lactone RNA RsmB Gac/Rsm |
| topic |
Bacterial Pectobacterium wasabiae ExpR2 AHL system Virulence N-Acyl homoserine lactone RNA RsmB Gac/Rsm |
| description |
This deposit is composed by the main article plus the supplementary materials of the publication. |
| publishDate |
2017 |
| dc.date.none.fl_str_mv |
2017-05-29T11:10:24Z 2017-05-23 2017-05-23T00:00:00Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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http://hdl.handle.net/10400.7/761 |
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http://hdl.handle.net/10400.7/761 |
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eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Valente RS, Nadal-Jimenez P, Carvalho AFP, Vieira FJD, Xavier KB. 2017. Signal integration in quorum sensing enables cross- species induction of virulence in Pectobacterium wasabiae. mBio 8:e00398-17. https://doi.org/10.1128/mBio.00398-17 . 10.1128/mBio.00398-17 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf application/pdf application/pdf application/pdf application/pdf application/pdf application/pdf application/pdf application/pdf application/pdf |
| dc.publisher.none.fl_str_mv |
American Society for Microbiology |
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American Society for Microbiology |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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