RUFY4 exists as two translationally regulated isoforms, that localize to the mitochondrion in activated macrophages

Detalhes bibliográficos
Autor(a) principal: Valečka, Jan
Data de Publicação: 2021
Outros Autores: Camosseto, Voahirana, McEwan, David G., Terawaki, Seigo, Liu, Zhuangzhuang, Strock, Eva, Almeida, Catarina R., Su, Bing, Dikic, Ivan, Liang, Yinming, Gatti, Evelina, Pierre, Philippe
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10773/37953
Resumo: We report here that RUFY4, a newly characterized member of the 'RUN and FYVE domain-containing' family of proteins previously associated with autophagy enhancement, is highly expressed in alveolar macrophages (AM). We show that RUFY4 interacts with mitochondria upon stimulation by microbial-associated molecular patterns of AM and dendritic cells. RUFY4 interaction with mitochondria and other organelles is dependent on a previously uncharacterized OmpH domain located immediately upstream of its C-terminal FYVE domain. Further, we demonstrate that rufy4 messenger RNA can be translated from an alternative translation initiation codon, giving rise to a N-terminally truncated form of the molecule lacking most of its RUN domain and with enhanced potential for its interaction with mitochondria. Our observations point towards a role of RUFY4 in selective mitochondria clearance in activated phagocytes.
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spelling RUFY4 exists as two translationally regulated isoforms, that localize to the mitochondrion in activated macrophagesRUFYMitophagySkpAlveolar macrophagesLPSWe report here that RUFY4, a newly characterized member of the 'RUN and FYVE domain-containing' family of proteins previously associated with autophagy enhancement, is highly expressed in alveolar macrophages (AM). We show that RUFY4 interacts with mitochondria upon stimulation by microbial-associated molecular patterns of AM and dendritic cells. RUFY4 interaction with mitochondria and other organelles is dependent on a previously uncharacterized OmpH domain located immediately upstream of its C-terminal FYVE domain. Further, we demonstrate that rufy4 messenger RNA can be translated from an alternative translation initiation codon, giving rise to a N-terminally truncated form of the molecule lacking most of its RUN domain and with enhanced potential for its interaction with mitochondria. Our observations point towards a role of RUFY4 in selective mitochondria clearance in activated phagocytes.The Royal Society2023-06-07T13:28:21Z2021-07-01T00:00:00Z2021-07info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10773/37953eng10.1098/rsos.202333Valečka, JanCamosseto, VoahiranaMcEwan, David G.Terawaki, SeigoLiu, ZhuangzhuangStrock, EvaAlmeida, Catarina R.Su, BingDikic, IvanLiang, YinmingGatti, EvelinaPierre, Philippeinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-22T12:11:54Zoai:ria.ua.pt:10773/37953Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:07:53.418560Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv RUFY4 exists as two translationally regulated isoforms, that localize to the mitochondrion in activated macrophages
title RUFY4 exists as two translationally regulated isoforms, that localize to the mitochondrion in activated macrophages
spellingShingle RUFY4 exists as two translationally regulated isoforms, that localize to the mitochondrion in activated macrophages
Valečka, Jan
RUFY
Mitophagy
Skp
Alveolar macrophages
LPS
title_short RUFY4 exists as two translationally regulated isoforms, that localize to the mitochondrion in activated macrophages
title_full RUFY4 exists as two translationally regulated isoforms, that localize to the mitochondrion in activated macrophages
title_fullStr RUFY4 exists as two translationally regulated isoforms, that localize to the mitochondrion in activated macrophages
title_full_unstemmed RUFY4 exists as two translationally regulated isoforms, that localize to the mitochondrion in activated macrophages
title_sort RUFY4 exists as two translationally regulated isoforms, that localize to the mitochondrion in activated macrophages
author Valečka, Jan
author_facet Valečka, Jan
Camosseto, Voahirana
McEwan, David G.
Terawaki, Seigo
Liu, Zhuangzhuang
Strock, Eva
Almeida, Catarina R.
Su, Bing
Dikic, Ivan
Liang, Yinming
Gatti, Evelina
Pierre, Philippe
author_role author
author2 Camosseto, Voahirana
McEwan, David G.
Terawaki, Seigo
Liu, Zhuangzhuang
Strock, Eva
Almeida, Catarina R.
Su, Bing
Dikic, Ivan
Liang, Yinming
Gatti, Evelina
Pierre, Philippe
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Valečka, Jan
Camosseto, Voahirana
McEwan, David G.
Terawaki, Seigo
Liu, Zhuangzhuang
Strock, Eva
Almeida, Catarina R.
Su, Bing
Dikic, Ivan
Liang, Yinming
Gatti, Evelina
Pierre, Philippe
dc.subject.por.fl_str_mv RUFY
Mitophagy
Skp
Alveolar macrophages
LPS
topic RUFY
Mitophagy
Skp
Alveolar macrophages
LPS
description We report here that RUFY4, a newly characterized member of the 'RUN and FYVE domain-containing' family of proteins previously associated with autophagy enhancement, is highly expressed in alveolar macrophages (AM). We show that RUFY4 interacts with mitochondria upon stimulation by microbial-associated molecular patterns of AM and dendritic cells. RUFY4 interaction with mitochondria and other organelles is dependent on a previously uncharacterized OmpH domain located immediately upstream of its C-terminal FYVE domain. Further, we demonstrate that rufy4 messenger RNA can be translated from an alternative translation initiation codon, giving rise to a N-terminally truncated form of the molecule lacking most of its RUN domain and with enhanced potential for its interaction with mitochondria. Our observations point towards a role of RUFY4 in selective mitochondria clearance in activated phagocytes.
publishDate 2021
dc.date.none.fl_str_mv 2021-07-01T00:00:00Z
2021-07
2023-06-07T13:28:21Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10773/37953
url http://hdl.handle.net/10773/37953
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1098/rsos.202333
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv The Royal Society
publisher.none.fl_str_mv The Royal Society
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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