Impact of the Pd2Spermine Chelate on Osteosarcoma Metabolism: An NMR Metabolomics Study

Detalhes bibliográficos
Autor(a) principal: Lamego, Inês
Data de Publicação: 2017
Outros Autores: Marques, M. Paula M., Duarte, Iola F., Martins, Ana S., Oliveira, Helena, Gil, Ana M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10316/45030
https://doi.org/10.1021/acs.jproteome.7b00035
Resumo: A metabolomics study of Pd2Spermine(Spm) on osteosarcoma MG-63 and osteoblastic HOb cells is presented to assess the impact of the potential palladium drug on cell metabolism compared with cisplatin (cDDP). Despite its higher cytotoxicity, Pd2Spm induced lower (and reversible) metabolic impact on MG-63 cells and the absence of apoptosis; conversely, it induced significant deviations in osteoblastic amino acid metabolism. However, when in combination with doxorubicin and methotrexate, Pd2Spm induced strong metabolic deviations on lipids, choline compounds, amino acids, nucleotides, and compounds related to antioxidative mechanisms (e.g., glutathione, inositol, hypoxanthine), similarly to the cDDP cocktail. Synergetic effects included triggering of lipid biosynthesis by Pd2Spm in the presence of doxorubicin (and reinforced by methotrexate) and changes in the glycosylation substrate uridine diphosphate acetylgalactosamine and methionine and serine metabolisms. This work provides promising results related to the impact of Pd2Spm on osteosarcoma cellular metabolism, particularly in drug combination protocols. Lipid metabolism, glycosylation, and amino acid metabolisms emerge as relevant features for targeted studies to further understand a potential anticancer mechanism of combined Pd2Spm.
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spelling Impact of the Pd2Spermine Chelate on Osteosarcoma Metabolism: An NMR Metabolomics StudyAmino AcidsAntineoplastic Combined Chemotherapy ProtocolsApoptosisCell Line, TumorCisplatinDoxorubicinGlycosylationHumansLipid MetabolismMagnetic Resonance SpectroscopyOsteosarcomaPalladiumSpermineMetabolomicsA metabolomics study of Pd2Spermine(Spm) on osteosarcoma MG-63 and osteoblastic HOb cells is presented to assess the impact of the potential palladium drug on cell metabolism compared with cisplatin (cDDP). Despite its higher cytotoxicity, Pd2Spm induced lower (and reversible) metabolic impact on MG-63 cells and the absence of apoptosis; conversely, it induced significant deviations in osteoblastic amino acid metabolism. However, when in combination with doxorubicin and methotrexate, Pd2Spm induced strong metabolic deviations on lipids, choline compounds, amino acids, nucleotides, and compounds related to antioxidative mechanisms (e.g., glutathione, inositol, hypoxanthine), similarly to the cDDP cocktail. Synergetic effects included triggering of lipid biosynthesis by Pd2Spm in the presence of doxorubicin (and reinforced by methotrexate) and changes in the glycosylation substrate uridine diphosphate acetylgalactosamine and methionine and serine metabolisms. This work provides promising results related to the impact of Pd2Spm on osteosarcoma cellular metabolism, particularly in drug combination protocols. Lipid metabolism, glycosylation, and amino acid metabolisms emerge as relevant features for targeted studies to further understand a potential anticancer mechanism of combined Pd2Spm.2017-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/45030http://hdl.handle.net/10316/45030https://doi.org/10.1021/acs.jproteome.7b00035engLamego, InêsMarques, M. Paula M.Duarte, Iola F.Martins, Ana S.Oliveira, HelenaGil, Ana M.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2021-09-22T08:28:18Zoai:estudogeral.uc.pt:10316/45030Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:56:07.439830Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Impact of the Pd2Spermine Chelate on Osteosarcoma Metabolism: An NMR Metabolomics Study
title Impact of the Pd2Spermine Chelate on Osteosarcoma Metabolism: An NMR Metabolomics Study
spellingShingle Impact of the Pd2Spermine Chelate on Osteosarcoma Metabolism: An NMR Metabolomics Study
Lamego, Inês
Amino Acids
Antineoplastic Combined Chemotherapy Protocols
Apoptosis
Cell Line, Tumor
Cisplatin
Doxorubicin
Glycosylation
Humans
Lipid Metabolism
Magnetic Resonance Spectroscopy
Osteosarcoma
Palladium
Spermine
Metabolomics
title_short Impact of the Pd2Spermine Chelate on Osteosarcoma Metabolism: An NMR Metabolomics Study
title_full Impact of the Pd2Spermine Chelate on Osteosarcoma Metabolism: An NMR Metabolomics Study
title_fullStr Impact of the Pd2Spermine Chelate on Osteosarcoma Metabolism: An NMR Metabolomics Study
title_full_unstemmed Impact of the Pd2Spermine Chelate on Osteosarcoma Metabolism: An NMR Metabolomics Study
title_sort Impact of the Pd2Spermine Chelate on Osteosarcoma Metabolism: An NMR Metabolomics Study
author Lamego, Inês
author_facet Lamego, Inês
Marques, M. Paula M.
Duarte, Iola F.
Martins, Ana S.
Oliveira, Helena
Gil, Ana M.
author_role author
author2 Marques, M. Paula M.
Duarte, Iola F.
Martins, Ana S.
Oliveira, Helena
Gil, Ana M.
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Lamego, Inês
Marques, M. Paula M.
Duarte, Iola F.
Martins, Ana S.
Oliveira, Helena
Gil, Ana M.
dc.subject.por.fl_str_mv Amino Acids
Antineoplastic Combined Chemotherapy Protocols
Apoptosis
Cell Line, Tumor
Cisplatin
Doxorubicin
Glycosylation
Humans
Lipid Metabolism
Magnetic Resonance Spectroscopy
Osteosarcoma
Palladium
Spermine
Metabolomics
topic Amino Acids
Antineoplastic Combined Chemotherapy Protocols
Apoptosis
Cell Line, Tumor
Cisplatin
Doxorubicin
Glycosylation
Humans
Lipid Metabolism
Magnetic Resonance Spectroscopy
Osteosarcoma
Palladium
Spermine
Metabolomics
description A metabolomics study of Pd2Spermine(Spm) on osteosarcoma MG-63 and osteoblastic HOb cells is presented to assess the impact of the potential palladium drug on cell metabolism compared with cisplatin (cDDP). Despite its higher cytotoxicity, Pd2Spm induced lower (and reversible) metabolic impact on MG-63 cells and the absence of apoptosis; conversely, it induced significant deviations in osteoblastic amino acid metabolism. However, when in combination with doxorubicin and methotrexate, Pd2Spm induced strong metabolic deviations on lipids, choline compounds, amino acids, nucleotides, and compounds related to antioxidative mechanisms (e.g., glutathione, inositol, hypoxanthine), similarly to the cDDP cocktail. Synergetic effects included triggering of lipid biosynthesis by Pd2Spm in the presence of doxorubicin (and reinforced by methotrexate) and changes in the glycosylation substrate uridine diphosphate acetylgalactosamine and methionine and serine metabolisms. This work provides promising results related to the impact of Pd2Spm on osteosarcoma cellular metabolism, particularly in drug combination protocols. Lipid metabolism, glycosylation, and amino acid metabolisms emerge as relevant features for targeted studies to further understand a potential anticancer mechanism of combined Pd2Spm.
publishDate 2017
dc.date.none.fl_str_mv 2017-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10316/45030
http://hdl.handle.net/10316/45030
https://doi.org/10.1021/acs.jproteome.7b00035
url http://hdl.handle.net/10316/45030
https://doi.org/10.1021/acs.jproteome.7b00035
dc.language.iso.fl_str_mv eng
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