Impact of the Pd2Spermine Chelate on Osteosarcoma Metabolism: An NMR Metabolomics Study
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/45030 https://doi.org/10.1021/acs.jproteome.7b00035 |
Resumo: | A metabolomics study of Pd2Spermine(Spm) on osteosarcoma MG-63 and osteoblastic HOb cells is presented to assess the impact of the potential palladium drug on cell metabolism compared with cisplatin (cDDP). Despite its higher cytotoxicity, Pd2Spm induced lower (and reversible) metabolic impact on MG-63 cells and the absence of apoptosis; conversely, it induced significant deviations in osteoblastic amino acid metabolism. However, when in combination with doxorubicin and methotrexate, Pd2Spm induced strong metabolic deviations on lipids, choline compounds, amino acids, nucleotides, and compounds related to antioxidative mechanisms (e.g., glutathione, inositol, hypoxanthine), similarly to the cDDP cocktail. Synergetic effects included triggering of lipid biosynthesis by Pd2Spm in the presence of doxorubicin (and reinforced by methotrexate) and changes in the glycosylation substrate uridine diphosphate acetylgalactosamine and methionine and serine metabolisms. This work provides promising results related to the impact of Pd2Spm on osteosarcoma cellular metabolism, particularly in drug combination protocols. Lipid metabolism, glycosylation, and amino acid metabolisms emerge as relevant features for targeted studies to further understand a potential anticancer mechanism of combined Pd2Spm. |
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Impact of the Pd2Spermine Chelate on Osteosarcoma Metabolism: An NMR Metabolomics StudyAmino AcidsAntineoplastic Combined Chemotherapy ProtocolsApoptosisCell Line, TumorCisplatinDoxorubicinGlycosylationHumansLipid MetabolismMagnetic Resonance SpectroscopyOsteosarcomaPalladiumSpermineMetabolomicsA metabolomics study of Pd2Spermine(Spm) on osteosarcoma MG-63 and osteoblastic HOb cells is presented to assess the impact of the potential palladium drug on cell metabolism compared with cisplatin (cDDP). Despite its higher cytotoxicity, Pd2Spm induced lower (and reversible) metabolic impact on MG-63 cells and the absence of apoptosis; conversely, it induced significant deviations in osteoblastic amino acid metabolism. However, when in combination with doxorubicin and methotrexate, Pd2Spm induced strong metabolic deviations on lipids, choline compounds, amino acids, nucleotides, and compounds related to antioxidative mechanisms (e.g., glutathione, inositol, hypoxanthine), similarly to the cDDP cocktail. Synergetic effects included triggering of lipid biosynthesis by Pd2Spm in the presence of doxorubicin (and reinforced by methotrexate) and changes in the glycosylation substrate uridine diphosphate acetylgalactosamine and methionine and serine metabolisms. This work provides promising results related to the impact of Pd2Spm on osteosarcoma cellular metabolism, particularly in drug combination protocols. Lipid metabolism, glycosylation, and amino acid metabolisms emerge as relevant features for targeted studies to further understand a potential anticancer mechanism of combined Pd2Spm.2017-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/45030http://hdl.handle.net/10316/45030https://doi.org/10.1021/acs.jproteome.7b00035engLamego, InêsMarques, M. Paula M.Duarte, Iola F.Martins, Ana S.Oliveira, HelenaGil, Ana M.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2021-09-22T08:28:18Zoai:estudogeral.uc.pt:10316/45030Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:56:07.439830Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Impact of the Pd2Spermine Chelate on Osteosarcoma Metabolism: An NMR Metabolomics Study |
title |
Impact of the Pd2Spermine Chelate on Osteosarcoma Metabolism: An NMR Metabolomics Study |
spellingShingle |
Impact of the Pd2Spermine Chelate on Osteosarcoma Metabolism: An NMR Metabolomics Study Lamego, Inês Amino Acids Antineoplastic Combined Chemotherapy Protocols Apoptosis Cell Line, Tumor Cisplatin Doxorubicin Glycosylation Humans Lipid Metabolism Magnetic Resonance Spectroscopy Osteosarcoma Palladium Spermine Metabolomics |
title_short |
Impact of the Pd2Spermine Chelate on Osteosarcoma Metabolism: An NMR Metabolomics Study |
title_full |
Impact of the Pd2Spermine Chelate on Osteosarcoma Metabolism: An NMR Metabolomics Study |
title_fullStr |
Impact of the Pd2Spermine Chelate on Osteosarcoma Metabolism: An NMR Metabolomics Study |
title_full_unstemmed |
Impact of the Pd2Spermine Chelate on Osteosarcoma Metabolism: An NMR Metabolomics Study |
title_sort |
Impact of the Pd2Spermine Chelate on Osteosarcoma Metabolism: An NMR Metabolomics Study |
author |
Lamego, Inês |
author_facet |
Lamego, Inês Marques, M. Paula M. Duarte, Iola F. Martins, Ana S. Oliveira, Helena Gil, Ana M. |
author_role |
author |
author2 |
Marques, M. Paula M. Duarte, Iola F. Martins, Ana S. Oliveira, Helena Gil, Ana M. |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Lamego, Inês Marques, M. Paula M. Duarte, Iola F. Martins, Ana S. Oliveira, Helena Gil, Ana M. |
dc.subject.por.fl_str_mv |
Amino Acids Antineoplastic Combined Chemotherapy Protocols Apoptosis Cell Line, Tumor Cisplatin Doxorubicin Glycosylation Humans Lipid Metabolism Magnetic Resonance Spectroscopy Osteosarcoma Palladium Spermine Metabolomics |
topic |
Amino Acids Antineoplastic Combined Chemotherapy Protocols Apoptosis Cell Line, Tumor Cisplatin Doxorubicin Glycosylation Humans Lipid Metabolism Magnetic Resonance Spectroscopy Osteosarcoma Palladium Spermine Metabolomics |
description |
A metabolomics study of Pd2Spermine(Spm) on osteosarcoma MG-63 and osteoblastic HOb cells is presented to assess the impact of the potential palladium drug on cell metabolism compared with cisplatin (cDDP). Despite its higher cytotoxicity, Pd2Spm induced lower (and reversible) metabolic impact on MG-63 cells and the absence of apoptosis; conversely, it induced significant deviations in osteoblastic amino acid metabolism. However, when in combination with doxorubicin and methotrexate, Pd2Spm induced strong metabolic deviations on lipids, choline compounds, amino acids, nucleotides, and compounds related to antioxidative mechanisms (e.g., glutathione, inositol, hypoxanthine), similarly to the cDDP cocktail. Synergetic effects included triggering of lipid biosynthesis by Pd2Spm in the presence of doxorubicin (and reinforced by methotrexate) and changes in the glycosylation substrate uridine diphosphate acetylgalactosamine and methionine and serine metabolisms. This work provides promising results related to the impact of Pd2Spm on osteosarcoma cellular metabolism, particularly in drug combination protocols. Lipid metabolism, glycosylation, and amino acid metabolisms emerge as relevant features for targeted studies to further understand a potential anticancer mechanism of combined Pd2Spm. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/45030 http://hdl.handle.net/10316/45030 https://doi.org/10.1021/acs.jproteome.7b00035 |
url |
http://hdl.handle.net/10316/45030 https://doi.org/10.1021/acs.jproteome.7b00035 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1817553617994907648 |