Antiproliferative activity of fucan nanogel

Detalhes bibliográficos
Autor(a) principal: Santos, Nednaldo Dantas
Data de Publicação: 2012
Outros Autores: Lima, Jailma Almeida, Vidal, Arthur Anthunes Jacome, Oliveira, Ruth Medeiros, Pedrosa, Sílvia Santos, Pereira, Paula, Gama, F. M., Rocha, Hugo Alexandre Oliveira, Gomes, Dayanne Lopes
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/22278
Resumo: Sulfated fucans comprise families of polydisperse natural polysaccharides based on sulfated l-fucose. Our aim was to investigate whether fucan nanogel induces cell-specific responses. To that end, a non toxic fucan extracted from Spatoglossum schröederi was chemically modified by grafting hexadecylamine to the polymer hydrophilic backbone. The resulting modified material (SNFuc) formed nanosized particles. The degree of substitution with hydrophobic chains was close to 100%, as estimated by elemental analysis. SNFfuc in aqueous media had a mean diameter of 123 nm and zeta potential of −38.3 ± 0.74 mV, as measured by dynamic light scattering. Nanoparticles conserved their size for up to 70 days. SNFuc cytotoxicity was determined using the MTT assay after culturing different cell lines for 24 h. Tumor-cell (HepG2, 786, H-S5) proliferation was inhibited by 2.0%–43.7% at nanogel concentrations of 0.05–0.5 mg/mL and rabbit aorta endothelial cells (RAEC) non-tumor cell line proliferation displayed inhibition of 8.0%–22.0%. On the other hand, nanogel improved Chinese hamster ovary (CHO) and monocyte macrophage cell (RAW) non-tumor cell line proliferation in the same concentration range. The antiproliferative effect against tumor cells was also confirmed using the BrdU test. Flow cytometric analysis revealed that the fucan nanogel inhibited 786 cell proliferation through caspase and caspase-independent mechanisms. In addition, SNFuc blocks 786 cell passages in the S and G2-M phases of the cell cycle.
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spelling Antiproliferative activity of fucan nanogelNanogelFucanNanogelsCancer cellsSulfated polysaccharidesSpatoglossum schröederiSpatoglossum schroederiScience & TechnologySulfated fucans comprise families of polydisperse natural polysaccharides based on sulfated l-fucose. Our aim was to investigate whether fucan nanogel induces cell-specific responses. To that end, a non toxic fucan extracted from Spatoglossum schröederi was chemically modified by grafting hexadecylamine to the polymer hydrophilic backbone. The resulting modified material (SNFuc) formed nanosized particles. The degree of substitution with hydrophobic chains was close to 100%, as estimated by elemental analysis. SNFfuc in aqueous media had a mean diameter of 123 nm and zeta potential of −38.3 ± 0.74 mV, as measured by dynamic light scattering. Nanoparticles conserved their size for up to 70 days. SNFuc cytotoxicity was determined using the MTT assay after culturing different cell lines for 24 h. Tumor-cell (HepG2, 786, H-S5) proliferation was inhibited by 2.0%–43.7% at nanogel concentrations of 0.05–0.5 mg/mL and rabbit aorta endothelial cells (RAEC) non-tumor cell line proliferation displayed inhibition of 8.0%–22.0%. On the other hand, nanogel improved Chinese hamster ovary (CHO) and monocyte macrophage cell (RAW) non-tumor cell line proliferation in the same concentration range. The antiproliferative effect against tumor cells was also confirmed using the BrdU test. Flow cytometric analysis revealed that the fucan nanogel inhibited 786 cell proliferation through caspase and caspase-independent mechanisms. In addition, SNFuc blocks 786 cell passages in the S and G2-M phases of the cell cycle.The present study was supported by CAPES, MCT, FAPERN/CNPq and CNPq, Brazil, as well as FCT, Portugal. N Dantas-Santos, J Almeida-Lima, AAJ Vidal, HAO Rocha are grateful to the CNPq and CAPES for their fellowship support. This research was submitted to the Graduate Program in Health Sciences at the Federal University of Rio Grande do Norte as part of the D.Sc. thesis of ND-S.MDPIUniversidade do MinhoSantos, Nednaldo DantasLima, Jailma AlmeidaVidal, Arthur Anthunes JacomeOliveira, Ruth MedeirosPedrosa, Sílvia SantosPereira, PaulaGama, F. M.Rocha, Hugo Alexandre OliveiraGomes, Dayanne Lopes20122012-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/22278eng1660-339710.3390/md1009200223118717info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:39:54Zoai:repositorium.sdum.uminho.pt:1822/22278Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:36:37.155006Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Antiproliferative activity of fucan nanogel
title Antiproliferative activity of fucan nanogel
spellingShingle Antiproliferative activity of fucan nanogel
Santos, Nednaldo Dantas
NanogelFucan
Nanogels
Cancer cells
Sulfated polysaccharides
Spatoglossum schröederi
Spatoglossum schroederi
Science & Technology
title_short Antiproliferative activity of fucan nanogel
title_full Antiproliferative activity of fucan nanogel
title_fullStr Antiproliferative activity of fucan nanogel
title_full_unstemmed Antiproliferative activity of fucan nanogel
title_sort Antiproliferative activity of fucan nanogel
author Santos, Nednaldo Dantas
author_facet Santos, Nednaldo Dantas
Lima, Jailma Almeida
Vidal, Arthur Anthunes Jacome
Oliveira, Ruth Medeiros
Pedrosa, Sílvia Santos
Pereira, Paula
Gama, F. M.
Rocha, Hugo Alexandre Oliveira
Gomes, Dayanne Lopes
author_role author
author2 Lima, Jailma Almeida
Vidal, Arthur Anthunes Jacome
Oliveira, Ruth Medeiros
Pedrosa, Sílvia Santos
Pereira, Paula
Gama, F. M.
Rocha, Hugo Alexandre Oliveira
Gomes, Dayanne Lopes
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Santos, Nednaldo Dantas
Lima, Jailma Almeida
Vidal, Arthur Anthunes Jacome
Oliveira, Ruth Medeiros
Pedrosa, Sílvia Santos
Pereira, Paula
Gama, F. M.
Rocha, Hugo Alexandre Oliveira
Gomes, Dayanne Lopes
dc.subject.por.fl_str_mv NanogelFucan
Nanogels
Cancer cells
Sulfated polysaccharides
Spatoglossum schröederi
Spatoglossum schroederi
Science & Technology
topic NanogelFucan
Nanogels
Cancer cells
Sulfated polysaccharides
Spatoglossum schröederi
Spatoglossum schroederi
Science & Technology
description Sulfated fucans comprise families of polydisperse natural polysaccharides based on sulfated l-fucose. Our aim was to investigate whether fucan nanogel induces cell-specific responses. To that end, a non toxic fucan extracted from Spatoglossum schröederi was chemically modified by grafting hexadecylamine to the polymer hydrophilic backbone. The resulting modified material (SNFuc) formed nanosized particles. The degree of substitution with hydrophobic chains was close to 100%, as estimated by elemental analysis. SNFfuc in aqueous media had a mean diameter of 123 nm and zeta potential of −38.3 ± 0.74 mV, as measured by dynamic light scattering. Nanoparticles conserved their size for up to 70 days. SNFuc cytotoxicity was determined using the MTT assay after culturing different cell lines for 24 h. Tumor-cell (HepG2, 786, H-S5) proliferation was inhibited by 2.0%–43.7% at nanogel concentrations of 0.05–0.5 mg/mL and rabbit aorta endothelial cells (RAEC) non-tumor cell line proliferation displayed inhibition of 8.0%–22.0%. On the other hand, nanogel improved Chinese hamster ovary (CHO) and monocyte macrophage cell (RAW) non-tumor cell line proliferation in the same concentration range. The antiproliferative effect against tumor cells was also confirmed using the BrdU test. Flow cytometric analysis revealed that the fucan nanogel inhibited 786 cell proliferation through caspase and caspase-independent mechanisms. In addition, SNFuc blocks 786 cell passages in the S and G2-M phases of the cell cycle.
publishDate 2012
dc.date.none.fl_str_mv 2012
2012-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/22278
url http://hdl.handle.net/1822/22278
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1660-3397
10.3390/md10092002
23118717
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dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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