Phagosome maturation during endosome interaction revealed by partial rhodopsin processing in retinal pigment epithelium
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10362/21960 |
Resumo: | Defects in phagocytosis and degradation of photoreceptor outer segments (POS) by the retinal pigment epithelium (RPE) are associated with aging and retinal disease. The daily burst of rod outer segment (ROS) phagocytosis by the RPE provides a unique opportunity to analyse phagosome processing in vivo. In mouse retinae, phagosomes containing stacked rhodopsin-rich discs were identified by immuno-electron microscopy. Early apical phagosomes stained with antibodies against both cytoplasmic and intradiscal domains of rhodopsin. During phagosome maturation, a remarkably synchronised loss of the cytoplasmic epitope coincided with movement to the cell body and preceded phagosome-lysosome fusion and disc degradation. Loss of the intradiscal rhodopsin epitope and disc digestion occurred upon fusion with cathepsin-D-positive lysosomes. The same sequential stages of phagosome maturation were identified in cultured RPE and macrophages challenged with isolated POS. Loss of the cytoplasmic rhodopsin epitope was insensitive to pH but sensitive to protease inhibition and coincided with the interaction of phagosomes with endosomes. Thus, during pre-lysosomal maturation of ROS-containing phagosomes, limited rhodopsin processing occurs upon interaction with endosomes. This potentially provides a sensitive readout of phagosome-endosome interactions that is applicable to multiple phagocytes. |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Phagosome maturation during endosome interaction revealed by partial rhodopsin processing in retinal pigment epitheliumPHAGOCYTOSISRPEENDOCYTIC PATHWAYUSHER-SYNDROME 1BAGE-RELATED-CHANGESCELL BIOLOGYPHOSPHATIDYLINOSITOL 3-KINASERhodopsinCATHEPSIN-DROD OUTER SEGMENTSMDCK CELLSPhagosomeALPHA-V-BETA-5 INTEGRINRPEPhagosomeRhodopsinDefects in phagocytosis and degradation of photoreceptor outer segments (POS) by the retinal pigment epithelium (RPE) are associated with aging and retinal disease. The daily burst of rod outer segment (ROS) phagocytosis by the RPE provides a unique opportunity to analyse phagosome processing in vivo. In mouse retinae, phagosomes containing stacked rhodopsin-rich discs were identified by immuno-electron microscopy. Early apical phagosomes stained with antibodies against both cytoplasmic and intradiscal domains of rhodopsin. During phagosome maturation, a remarkably synchronised loss of the cytoplasmic epitope coincided with movement to the cell body and preceded phagosome-lysosome fusion and disc degradation. Loss of the intradiscal rhodopsin epitope and disc digestion occurred upon fusion with cathepsin-D-positive lysosomes. The same sequential stages of phagosome maturation were identified in cultured RPE and macrophages challenged with isolated POS. Loss of the cytoplasmic rhodopsin epitope was insensitive to pH but sensitive to protease inhibition and coincided with the interaction of phagosomes with endosomes. Thus, during pre-lysosomal maturation of ROS-containing phagosomes, limited rhodopsin processing occurs upon interaction with endosomes. This potentially provides a sensitive readout of phagosome-endosome interactions that is applicable to multiple phagocytes.Centro de Estudos de Doenças Crónicas (CEDOC)NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)RUNWavre-Shapton, Silène TMeschede, Ingrid PSeabra, Miguel CFutter, Clare E2017-07-14T22:01:02Z2014-09-012014-09-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10application/pdfhttp://hdl.handle.net/10362/21960eng0021-9533PURE: 398898https://doi.org/10.1242/jcs.154757info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-22T17:26:37Zoai:run.unl.pt:10362/21960Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-22T17:26:37Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Phagosome maturation during endosome interaction revealed by partial rhodopsin processing in retinal pigment epithelium |
title |
Phagosome maturation during endosome interaction revealed by partial rhodopsin processing in retinal pigment epithelium |
spellingShingle |
Phagosome maturation during endosome interaction revealed by partial rhodopsin processing in retinal pigment epithelium Wavre-Shapton, Silène T PHAGOCYTOSIS RPE ENDOCYTIC PATHWAY USHER-SYNDROME 1B AGE-RELATED-CHANGES CELL BIOLOGY PHOSPHATIDYLINOSITOL 3-KINASE Rhodopsin CATHEPSIN-D ROD OUTER SEGMENTS MDCK CELLS Phagosome ALPHA-V-BETA-5 INTEGRIN RPE Phagosome Rhodopsin |
title_short |
Phagosome maturation during endosome interaction revealed by partial rhodopsin processing in retinal pigment epithelium |
title_full |
Phagosome maturation during endosome interaction revealed by partial rhodopsin processing in retinal pigment epithelium |
title_fullStr |
Phagosome maturation during endosome interaction revealed by partial rhodopsin processing in retinal pigment epithelium |
title_full_unstemmed |
Phagosome maturation during endosome interaction revealed by partial rhodopsin processing in retinal pigment epithelium |
title_sort |
Phagosome maturation during endosome interaction revealed by partial rhodopsin processing in retinal pigment epithelium |
author |
Wavre-Shapton, Silène T |
author_facet |
Wavre-Shapton, Silène T Meschede, Ingrid P Seabra, Miguel C Futter, Clare E |
author_role |
author |
author2 |
Meschede, Ingrid P Seabra, Miguel C Futter, Clare E |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Centro de Estudos de Doenças Crónicas (CEDOC) NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM) RUN |
dc.contributor.author.fl_str_mv |
Wavre-Shapton, Silène T Meschede, Ingrid P Seabra, Miguel C Futter, Clare E |
dc.subject.por.fl_str_mv |
PHAGOCYTOSIS RPE ENDOCYTIC PATHWAY USHER-SYNDROME 1B AGE-RELATED-CHANGES CELL BIOLOGY PHOSPHATIDYLINOSITOL 3-KINASE Rhodopsin CATHEPSIN-D ROD OUTER SEGMENTS MDCK CELLS Phagosome ALPHA-V-BETA-5 INTEGRIN RPE Phagosome Rhodopsin |
topic |
PHAGOCYTOSIS RPE ENDOCYTIC PATHWAY USHER-SYNDROME 1B AGE-RELATED-CHANGES CELL BIOLOGY PHOSPHATIDYLINOSITOL 3-KINASE Rhodopsin CATHEPSIN-D ROD OUTER SEGMENTS MDCK CELLS Phagosome ALPHA-V-BETA-5 INTEGRIN RPE Phagosome Rhodopsin |
description |
Defects in phagocytosis and degradation of photoreceptor outer segments (POS) by the retinal pigment epithelium (RPE) are associated with aging and retinal disease. The daily burst of rod outer segment (ROS) phagocytosis by the RPE provides a unique opportunity to analyse phagosome processing in vivo. In mouse retinae, phagosomes containing stacked rhodopsin-rich discs were identified by immuno-electron microscopy. Early apical phagosomes stained with antibodies against both cytoplasmic and intradiscal domains of rhodopsin. During phagosome maturation, a remarkably synchronised loss of the cytoplasmic epitope coincided with movement to the cell body and preceded phagosome-lysosome fusion and disc degradation. Loss of the intradiscal rhodopsin epitope and disc digestion occurred upon fusion with cathepsin-D-positive lysosomes. The same sequential stages of phagosome maturation were identified in cultured RPE and macrophages challenged with isolated POS. Loss of the cytoplasmic rhodopsin epitope was insensitive to pH but sensitive to protease inhibition and coincided with the interaction of phagosomes with endosomes. Thus, during pre-lysosomal maturation of ROS-containing phagosomes, limited rhodopsin processing occurs upon interaction with endosomes. This potentially provides a sensitive readout of phagosome-endosome interactions that is applicable to multiple phagocytes. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-09-01 2014-09-01T00:00:00Z 2017-07-14T22:01:02Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/21960 |
url |
http://hdl.handle.net/10362/21960 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0021-9533 PURE: 398898 https://doi.org/10.1242/jcs.154757 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
10 application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
mluisa.alvim@gmail.com |
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1817545593718833152 |