On to the impact of low cost substrates for BNC production

Detalhes bibliográficos
Autor(a) principal: Rodrigues, Ana Cristina
Data de Publicação: 2019
Outros Autores: Soares da Silva, F. A. G., Oliveira, João V., Forte, Ana, Dourado, Fernando, Alves, M. M., Gama, F. M.
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/61752
Resumo: Bacterial nanocellulose (BNC) is an exopolysaccharide produced by certain acetic acid bacteria. It has high crystallinity, high mechanical strength, high purity and high water-holding capacity. These properties make it useful in making artificial skin (1), electronic paper, composite reinforcement, development of food and cosmetic applications (2). The cost of fermentation media is believed to contribute significantly to the operational costs, especially if synthetic commercial media are used. Hence, much research on BNC production using low-cost substrates has been done focusing on lowering the production costs (3). Also, to meet the requirement for industrial applications, effective large-scale BNC production systems need to be developed, which involves improving the fermentation conditions and identifying high yield BNC-producing strains (4). However, as with many fermentation systems, while promoting the recycling of low value-added products, the use of complex substrates may in fact represent a bottleneck in the BNC fermentation processes. Some of these substrates present, comparatively to synthetic nutrients, high chemical oxygen demand (COD), total and volatile solids (TS and VS), total nitrogen (TN), antimicrobial components (such as phenols) Consequently, these alternative substrates may place an economic problem either downhill, due to the need for wastewaters treatments and/or, uphill, due to the need of substrates pre-treatment. In this work, the optimization of alternative BNC culture medium (Molasses-Corn Steep Liquor, MOL-CSL), using Response surface methodology central composite design was used to evaluate the effect of inexpensive and widely available nutrients sources, namely MOL, ethanol (EtOH), CSL and ammonium sulphate on BNC production yield under static culture by komagataeibacter xylinus BPR 2001. The optimized parameters for maximum BNC production were: % (m/v): MOL 5.38, CSL 1.91, ammonium sulphate 0.63, disodium phosphate 0.270, citric acid 0.115 and ethanol 1.38 % (v/v). The maximum BNC production yield were 7.5 ± 0.54 g/L versus 1.79 ± 0.04 g/L for MOL-CSL and synthetic medium (HS-EtOH) culture medium, respectively. The resulting wastewater from each culture medium was characterized regarding COD, TN, TS and VS, leading to the conclusion that the wastewaters generated using MOL-CSL are more heavily charged with organic matter, increasing the final costs of BNC production due to the higher costs associated to wastewater treatment. Anaerobic digestion (AD) was studied for wastewater treatment and biogas production from the wastewaters of the BNC fermentation and purification process. Finally, a preliminary Life Cycle Assessment of BNC production was performed and will be presented.
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spelling On to the impact of low cost substrates for BNC productionEngenharia e Tecnologia::Biotecnologia IndustrialBacterial nanocellulose (BNC) is an exopolysaccharide produced by certain acetic acid bacteria. It has high crystallinity, high mechanical strength, high purity and high water-holding capacity. These properties make it useful in making artificial skin (1), electronic paper, composite reinforcement, development of food and cosmetic applications (2). The cost of fermentation media is believed to contribute significantly to the operational costs, especially if synthetic commercial media are used. Hence, much research on BNC production using low-cost substrates has been done focusing on lowering the production costs (3). Also, to meet the requirement for industrial applications, effective large-scale BNC production systems need to be developed, which involves improving the fermentation conditions and identifying high yield BNC-producing strains (4). However, as with many fermentation systems, while promoting the recycling of low value-added products, the use of complex substrates may in fact represent a bottleneck in the BNC fermentation processes. Some of these substrates present, comparatively to synthetic nutrients, high chemical oxygen demand (COD), total and volatile solids (TS and VS), total nitrogen (TN), antimicrobial components (such as phenols) Consequently, these alternative substrates may place an economic problem either downhill, due to the need for wastewaters treatments and/or, uphill, due to the need of substrates pre-treatment. In this work, the optimization of alternative BNC culture medium (Molasses-Corn Steep Liquor, MOL-CSL), using Response surface methodology central composite design was used to evaluate the effect of inexpensive and widely available nutrients sources, namely MOL, ethanol (EtOH), CSL and ammonium sulphate on BNC production yield under static culture by komagataeibacter xylinus BPR 2001. The optimized parameters for maximum BNC production were: % (m/v): MOL 5.38, CSL 1.91, ammonium sulphate 0.63, disodium phosphate 0.270, citric acid 0.115 and ethanol 1.38 % (v/v). The maximum BNC production yield were 7.5 ± 0.54 g/L versus 1.79 ± 0.04 g/L for MOL-CSL and synthetic medium (HS-EtOH) culture medium, respectively. The resulting wastewater from each culture medium was characterized regarding COD, TN, TS and VS, leading to the conclusion that the wastewaters generated using MOL-CSL are more heavily charged with organic matter, increasing the final costs of BNC production due to the higher costs associated to wastewater treatment. Anaerobic digestion (AD) was studied for wastewater treatment and biogas production from the wastewaters of the BNC fermentation and purification process. Finally, a preliminary Life Cycle Assessment of BNC production was performed and will be presented.info:eu-repo/semantics/publishedVersionUniversidade do MinhoRodrigues, Ana CristinaSoares da Silva, F. A. G.Oliveira, João V.Forte, AnaDourado, FernandoAlves, M. M.Gama, F. M.2019-10-032019-10-03T00:00:00Zconference objectinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/1822/61752engRodrigues, Ana Cristina; Soares da Silva, F. A. G.; Oliveira, João V.; Forte, Ana; Dourado, Fernando; Alves, M. Madalena; Gama, F. M., On to the impact of low cost substrates for BNC production. ISBNC 2019 - 4th International Symposium on Bacterial Nanocellulose. Porto, Portugal, Oct 3-4, 2019.http://www.4isbnc.com/Book%20of%20Abstracts_4ISBNC.pdfinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-11T06:10:52Zoai:repositorium.sdum.uminho.pt:1822/61752Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-11T06:10:52Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv On to the impact of low cost substrates for BNC production
title On to the impact of low cost substrates for BNC production
spellingShingle On to the impact of low cost substrates for BNC production
Rodrigues, Ana Cristina
Engenharia e Tecnologia::Biotecnologia Industrial
title_short On to the impact of low cost substrates for BNC production
title_full On to the impact of low cost substrates for BNC production
title_fullStr On to the impact of low cost substrates for BNC production
title_full_unstemmed On to the impact of low cost substrates for BNC production
title_sort On to the impact of low cost substrates for BNC production
author Rodrigues, Ana Cristina
author_facet Rodrigues, Ana Cristina
Soares da Silva, F. A. G.
Oliveira, João V.
Forte, Ana
Dourado, Fernando
Alves, M. M.
Gama, F. M.
author_role author
author2 Soares da Silva, F. A. G.
Oliveira, João V.
Forte, Ana
Dourado, Fernando
Alves, M. M.
Gama, F. M.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Rodrigues, Ana Cristina
Soares da Silva, F. A. G.
Oliveira, João V.
Forte, Ana
Dourado, Fernando
Alves, M. M.
Gama, F. M.
dc.subject.por.fl_str_mv Engenharia e Tecnologia::Biotecnologia Industrial
topic Engenharia e Tecnologia::Biotecnologia Industrial
description Bacterial nanocellulose (BNC) is an exopolysaccharide produced by certain acetic acid bacteria. It has high crystallinity, high mechanical strength, high purity and high water-holding capacity. These properties make it useful in making artificial skin (1), electronic paper, composite reinforcement, development of food and cosmetic applications (2). The cost of fermentation media is believed to contribute significantly to the operational costs, especially if synthetic commercial media are used. Hence, much research on BNC production using low-cost substrates has been done focusing on lowering the production costs (3). Also, to meet the requirement for industrial applications, effective large-scale BNC production systems need to be developed, which involves improving the fermentation conditions and identifying high yield BNC-producing strains (4). However, as with many fermentation systems, while promoting the recycling of low value-added products, the use of complex substrates may in fact represent a bottleneck in the BNC fermentation processes. Some of these substrates present, comparatively to synthetic nutrients, high chemical oxygen demand (COD), total and volatile solids (TS and VS), total nitrogen (TN), antimicrobial components (such as phenols) Consequently, these alternative substrates may place an economic problem either downhill, due to the need for wastewaters treatments and/or, uphill, due to the need of substrates pre-treatment. In this work, the optimization of alternative BNC culture medium (Molasses-Corn Steep Liquor, MOL-CSL), using Response surface methodology central composite design was used to evaluate the effect of inexpensive and widely available nutrients sources, namely MOL, ethanol (EtOH), CSL and ammonium sulphate on BNC production yield under static culture by komagataeibacter xylinus BPR 2001. The optimized parameters for maximum BNC production were: % (m/v): MOL 5.38, CSL 1.91, ammonium sulphate 0.63, disodium phosphate 0.270, citric acid 0.115 and ethanol 1.38 % (v/v). The maximum BNC production yield were 7.5 ± 0.54 g/L versus 1.79 ± 0.04 g/L for MOL-CSL and synthetic medium (HS-EtOH) culture medium, respectively. The resulting wastewater from each culture medium was characterized regarding COD, TN, TS and VS, leading to the conclusion that the wastewaters generated using MOL-CSL are more heavily charged with organic matter, increasing the final costs of BNC production due to the higher costs associated to wastewater treatment. Anaerobic digestion (AD) was studied for wastewater treatment and biogas production from the wastewaters of the BNC fermentation and purification process. Finally, a preliminary Life Cycle Assessment of BNC production was performed and will be presented.
publishDate 2019
dc.date.none.fl_str_mv 2019-10-03
2019-10-03T00:00:00Z
dc.type.driver.fl_str_mv conference object
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/61752
url http://hdl.handle.net/1822/61752
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Rodrigues, Ana Cristina; Soares da Silva, F. A. G.; Oliveira, João V.; Forte, Ana; Dourado, Fernando; Alves, M. Madalena; Gama, F. M., On to the impact of low cost substrates for BNC production. ISBNC 2019 - 4th International Symposium on Bacterial Nanocellulose. Porto, Portugal, Oct 3-4, 2019.
http://www.4isbnc.com/Book%20of%20Abstracts_4ISBNC.pdf
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eu_rights_str_mv openAccess
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dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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