Differential survival of Saccharomyces cerevisiae UE-ME3 and BY4741 strains to titanium dioxide nanoparticles depend on glutathione level increase and Catalase T induction.
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10174/7837 https://doi.org/10.1016/j.cbpa.2012.05.053 |
Resumo: | Nanotechnology has released materials into the environment whose action on living organisms is poorly known and that is urgent clarify. Some authors have shown that these materials behave as ROS generators, reason which justifies its use in studies of stress. In general, the cell health is correlated with elevated values of GSH/GSSG ratio, a marker of stability linked to a highly reducing environment. Changes in this parameter are frequently used as markers of oxidative stress, sometimes responsible for the inducing of cell growth and differentiation or in the activation of signaling pathways and cell death. The wild-type S. cerevisiae UE-ME3 strain isolated from the musts of Alentejo, Portugal, was used in this work in order to detect singular physiological properties that facilitate their survival in extreme environments for the life, comparing with the response exhibited by S. cerevisiae BY4741, a strain belonging to the Eurocast collection extensively used in physiological and biochemical studies of stress. Cells growing at mid exponential phase in liquid YEPD medium with 2 % (w/v) glucose, at 25 ºC, were exposed during 200 min to 0.1 or 1.0 µg/mL of titanium dioxide nanoparticles (TiO2-NP), prepared by sonication, at same temperature conditions. Samples of each treatment were used to obtain the post-12000 g supernatant for determination of proteins, glutathione, ROS and MDA contents as well as CAT T activity. The results show that UE-ME3 strain, at 25 °C, has greater capacity to adapt to TiO2-NP presence in the culture media than demonstrated by the BY4741 strain, since the protein and glutathione content in UE-ME3 strain was significantly highest in either treatment. It´s probable that adaptive response to titanium nanoparticles detected in the UE-ME3 strain, partly depends on antioxidant activity mediated by GSH/GSSG ratio and CAT T activity, significantly highest in the wild-type strain which protect the cells from the ROS generation by TiO2-NP. |
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Differential survival of Saccharomyces cerevisiae UE-ME3 and BY4741 strains to titanium dioxide nanoparticles depend on glutathione level increase and Catalase T induction.glutathioneROSMDA contentsNanotechnology has released materials into the environment whose action on living organisms is poorly known and that is urgent clarify. Some authors have shown that these materials behave as ROS generators, reason which justifies its use in studies of stress. In general, the cell health is correlated with elevated values of GSH/GSSG ratio, a marker of stability linked to a highly reducing environment. Changes in this parameter are frequently used as markers of oxidative stress, sometimes responsible for the inducing of cell growth and differentiation or in the activation of signaling pathways and cell death. The wild-type S. cerevisiae UE-ME3 strain isolated from the musts of Alentejo, Portugal, was used in this work in order to detect singular physiological properties that facilitate their survival in extreme environments for the life, comparing with the response exhibited by S. cerevisiae BY4741, a strain belonging to the Eurocast collection extensively used in physiological and biochemical studies of stress. Cells growing at mid exponential phase in liquid YEPD medium with 2 % (w/v) glucose, at 25 ºC, were exposed during 200 min to 0.1 or 1.0 µg/mL of titanium dioxide nanoparticles (TiO2-NP), prepared by sonication, at same temperature conditions. Samples of each treatment were used to obtain the post-12000 g supernatant for determination of proteins, glutathione, ROS and MDA contents as well as CAT T activity. The results show that UE-ME3 strain, at 25 °C, has greater capacity to adapt to TiO2-NP presence in the culture media than demonstrated by the BY4741 strain, since the protein and glutathione content in UE-ME3 strain was significantly highest in either treatment. It´s probable that adaptive response to titanium nanoparticles detected in the UE-ME3 strain, partly depends on antioxidant activity mediated by GSH/GSSG ratio and CAT T activity, significantly highest in the wild-type strain which protect the cells from the ROS generation by TiO2-NP.Elsevier2013-01-28T16:35:29Z2013-01-282012-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10174/7837http://hdl.handle.net/10174/7837https://doi.org/10.1016/j.cbpa.2012.05.053engCapela-Pires J, Ferreira R, Alves-Pereira I (2012) Differential survival of Saccharomyces cerevisiae UE-ME3 and BY4741 strains to titanium dioxide nanoparticles depend on glutathione level increase and Catalase T induction. Comparative Biochemistry and physiology - Part A: molecular & integrative physiology (CBA), Part A 163:S17ICAAMndraf@uevora.ptiap@uevora.pt548Capela-Pires, JFerreira, RuiAlves-Pereira, Isabelinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T18:48:21Zoai:dspace.uevora.pt:10174/7837Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:02:15.715506Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Differential survival of Saccharomyces cerevisiae UE-ME3 and BY4741 strains to titanium dioxide nanoparticles depend on glutathione level increase and Catalase T induction. |
title |
Differential survival of Saccharomyces cerevisiae UE-ME3 and BY4741 strains to titanium dioxide nanoparticles depend on glutathione level increase and Catalase T induction. |
spellingShingle |
Differential survival of Saccharomyces cerevisiae UE-ME3 and BY4741 strains to titanium dioxide nanoparticles depend on glutathione level increase and Catalase T induction. Capela-Pires, J glutathione ROS MDA contents |
title_short |
Differential survival of Saccharomyces cerevisiae UE-ME3 and BY4741 strains to titanium dioxide nanoparticles depend on glutathione level increase and Catalase T induction. |
title_full |
Differential survival of Saccharomyces cerevisiae UE-ME3 and BY4741 strains to titanium dioxide nanoparticles depend on glutathione level increase and Catalase T induction. |
title_fullStr |
Differential survival of Saccharomyces cerevisiae UE-ME3 and BY4741 strains to titanium dioxide nanoparticles depend on glutathione level increase and Catalase T induction. |
title_full_unstemmed |
Differential survival of Saccharomyces cerevisiae UE-ME3 and BY4741 strains to titanium dioxide nanoparticles depend on glutathione level increase and Catalase T induction. |
title_sort |
Differential survival of Saccharomyces cerevisiae UE-ME3 and BY4741 strains to titanium dioxide nanoparticles depend on glutathione level increase and Catalase T induction. |
author |
Capela-Pires, J |
author_facet |
Capela-Pires, J Ferreira, Rui Alves-Pereira, Isabel |
author_role |
author |
author2 |
Ferreira, Rui Alves-Pereira, Isabel |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Capela-Pires, J Ferreira, Rui Alves-Pereira, Isabel |
dc.subject.por.fl_str_mv |
glutathione ROS MDA contents |
topic |
glutathione ROS MDA contents |
description |
Nanotechnology has released materials into the environment whose action on living organisms is poorly known and that is urgent clarify. Some authors have shown that these materials behave as ROS generators, reason which justifies its use in studies of stress. In general, the cell health is correlated with elevated values of GSH/GSSG ratio, a marker of stability linked to a highly reducing environment. Changes in this parameter are frequently used as markers of oxidative stress, sometimes responsible for the inducing of cell growth and differentiation or in the activation of signaling pathways and cell death. The wild-type S. cerevisiae UE-ME3 strain isolated from the musts of Alentejo, Portugal, was used in this work in order to detect singular physiological properties that facilitate their survival in extreme environments for the life, comparing with the response exhibited by S. cerevisiae BY4741, a strain belonging to the Eurocast collection extensively used in physiological and biochemical studies of stress. Cells growing at mid exponential phase in liquid YEPD medium with 2 % (w/v) glucose, at 25 ºC, were exposed during 200 min to 0.1 or 1.0 µg/mL of titanium dioxide nanoparticles (TiO2-NP), prepared by sonication, at same temperature conditions. Samples of each treatment were used to obtain the post-12000 g supernatant for determination of proteins, glutathione, ROS and MDA contents as well as CAT T activity. The results show that UE-ME3 strain, at 25 °C, has greater capacity to adapt to TiO2-NP presence in the culture media than demonstrated by the BY4741 strain, since the protein and glutathione content in UE-ME3 strain was significantly highest in either treatment. It´s probable that adaptive response to titanium nanoparticles detected in the UE-ME3 strain, partly depends on antioxidant activity mediated by GSH/GSSG ratio and CAT T activity, significantly highest in the wild-type strain which protect the cells from the ROS generation by TiO2-NP. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-01-01T00:00:00Z 2013-01-28T16:35:29Z 2013-01-28 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10174/7837 http://hdl.handle.net/10174/7837 https://doi.org/10.1016/j.cbpa.2012.05.053 |
url |
http://hdl.handle.net/10174/7837 https://doi.org/10.1016/j.cbpa.2012.05.053 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Capela-Pires J, Ferreira R, Alves-Pereira I (2012) Differential survival of Saccharomyces cerevisiae UE-ME3 and BY4741 strains to titanium dioxide nanoparticles depend on glutathione level increase and Catalase T induction. Comparative Biochemistry and physiology - Part A: molecular & integrative physiology (CBA), Part A 163:S17 ICAAM nd raf@uevora.pt iap@uevora.pt 548 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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