Pseudomonas associated with Bursaphelenchus xylophilus, its insect vector and the host tree: A role in pine wilt disease?
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10174/27553 https://doi.org/10.1111/efp.12564 |
Resumo: | In this study, we characterized the diversity of Pseudomonas associated with Bursaphelenchus xylophilus, its insect vector (Monochamus galloprovincialis) and its host (Pinus pinaster), by a culture‐independent approach using rpoD clone libraries. Clone libraries of Pseudomonas rpoD were obtained from B. xylophilus, M. galloprovincialis and infected P. pinaster. Most M. galloprovincialis and B. xylophilus sequences grouped together in the P. fluorescens group. Genes related to xenobiotics degradation and phenylacetate synthesis were present in the genomes of the type strains closely related to sequences retrieved from the nematode libraries. Results demonstrated that the nematode, during its life stages inside the tree, maintains a diverse Pseudomonas community that is closely related to the one associated with the insect vector. These bacteria might contribute to degradation of xenobiotics and tree weakening during the nematode tree infection. |
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Pseudomonas associated with Bursaphelenchus xylophilus, its insect vector and the host tree: A role in pine wilt disease?Bursaphelenchus xylophilusPseudomonasIn this study, we characterized the diversity of Pseudomonas associated with Bursaphelenchus xylophilus, its insect vector (Monochamus galloprovincialis) and its host (Pinus pinaster), by a culture‐independent approach using rpoD clone libraries. Clone libraries of Pseudomonas rpoD were obtained from B. xylophilus, M. galloprovincialis and infected P. pinaster. Most M. galloprovincialis and B. xylophilus sequences grouped together in the P. fluorescens group. Genes related to xenobiotics degradation and phenylacetate synthesis were present in the genomes of the type strains closely related to sequences retrieved from the nematode libraries. Results demonstrated that the nematode, during its life stages inside the tree, maintains a diverse Pseudomonas community that is closely related to the one associated with the insect vector. These bacteria might contribute to degradation of xenobiotics and tree weakening during the nematode tree infection.Wiley2020-03-02T11:31:06Z2020-03-022019-10-14T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10174/27553http://hdl.handle.net/10174/27553https://doi.org/10.1111/efp.12564porAlves, M., C. Vicente, I. Henriques, M. Mota, A. Pereira. 2019. Pseudomonas associated with Bursaphelenchus xylophilus, its insect vector and the host tree: a role in pine wilt disease?. Forest Pathology. DOI: 10.1111/efp.12564.martasalves@ua.pt578Alves, Martainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T19:23:10Zoai:dspace.uevora.pt:10174/27553Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:17:37.978262Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Pseudomonas associated with Bursaphelenchus xylophilus, its insect vector and the host tree: A role in pine wilt disease? |
title |
Pseudomonas associated with Bursaphelenchus xylophilus, its insect vector and the host tree: A role in pine wilt disease? |
spellingShingle |
Pseudomonas associated with Bursaphelenchus xylophilus, its insect vector and the host tree: A role in pine wilt disease? Alves, Marta Bursaphelenchus xylophilus Pseudomonas |
title_short |
Pseudomonas associated with Bursaphelenchus xylophilus, its insect vector and the host tree: A role in pine wilt disease? |
title_full |
Pseudomonas associated with Bursaphelenchus xylophilus, its insect vector and the host tree: A role in pine wilt disease? |
title_fullStr |
Pseudomonas associated with Bursaphelenchus xylophilus, its insect vector and the host tree: A role in pine wilt disease? |
title_full_unstemmed |
Pseudomonas associated with Bursaphelenchus xylophilus, its insect vector and the host tree: A role in pine wilt disease? |
title_sort |
Pseudomonas associated with Bursaphelenchus xylophilus, its insect vector and the host tree: A role in pine wilt disease? |
author |
Alves, Marta |
author_facet |
Alves, Marta |
author_role |
author |
dc.contributor.author.fl_str_mv |
Alves, Marta |
dc.subject.por.fl_str_mv |
Bursaphelenchus xylophilus Pseudomonas |
topic |
Bursaphelenchus xylophilus Pseudomonas |
description |
In this study, we characterized the diversity of Pseudomonas associated with Bursaphelenchus xylophilus, its insect vector (Monochamus galloprovincialis) and its host (Pinus pinaster), by a culture‐independent approach using rpoD clone libraries. Clone libraries of Pseudomonas rpoD were obtained from B. xylophilus, M. galloprovincialis and infected P. pinaster. Most M. galloprovincialis and B. xylophilus sequences grouped together in the P. fluorescens group. Genes related to xenobiotics degradation and phenylacetate synthesis were present in the genomes of the type strains closely related to sequences retrieved from the nematode libraries. Results demonstrated that the nematode, during its life stages inside the tree, maintains a diverse Pseudomonas community that is closely related to the one associated with the insect vector. These bacteria might contribute to degradation of xenobiotics and tree weakening during the nematode tree infection. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-14T00:00:00Z 2020-03-02T11:31:06Z 2020-03-02 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10174/27553 http://hdl.handle.net/10174/27553 https://doi.org/10.1111/efp.12564 |
url |
http://hdl.handle.net/10174/27553 https://doi.org/10.1111/efp.12564 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
Alves, M., C. Vicente, I. Henriques, M. Mota, A. Pereira. 2019. Pseudomonas associated with Bursaphelenchus xylophilus, its insect vector and the host tree: a role in pine wilt disease?. Forest Pathology. DOI: 10.1111/efp.12564. martasalves@ua.pt 578 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Wiley |
publisher.none.fl_str_mv |
Wiley |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799136658549899264 |