Improvement of biosurfactant production by Wickerhamomyces anomalus CCMA 0358 and its potential application in bioremediation
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/1822/50467 |
Resumo: | In this work, biosurfactant production by Wickerhamomyces anomalus CCMA 0358 was increased through the development of an optimized culture medium using response surface methodology. The optimized culture medium contained yeast extract (4.64 g/L), ammonium sulfate (4.22 g/L), glucose (1.39 g/L) and olive oil (10 g/L). Biosurfactant production using this medium was validated both in flasks and bioreactor, and the surface tension was reduced from 49.0 mN/m up to 31.4 mN/m and 29.3 mN/m, respectively. In both cases, the highest biosurfactant production was achieved after 24 h of growth. W. anomalus CCMA 0358 demonstrated to be a fast biosurfactant producer (24 h) as compared to other yeast strains previously reported (144-240 h). The produced biosurfactant remained stable at high temperature (121ºC), NaCl concentrations as high as 300 g/L, and pH values between 6 and 12. The crude biosurfactant allowed the recovery of 20% of crude oil from contaminated sand, being a promising candidate for application in bioremediation or in the petroleum industry. |
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Improvement of biosurfactant production by Wickerhamomyces anomalus CCMA 0358 and its potential application in bioremediationWickerhamomyces anomalusBiosurfactantBioreactorResponse surface methodologyBioremediationScience & TechnologyIn this work, biosurfactant production by Wickerhamomyces anomalus CCMA 0358 was increased through the development of an optimized culture medium using response surface methodology. The optimized culture medium contained yeast extract (4.64 g/L), ammonium sulfate (4.22 g/L), glucose (1.39 g/L) and olive oil (10 g/L). Biosurfactant production using this medium was validated both in flasks and bioreactor, and the surface tension was reduced from 49.0 mN/m up to 31.4 mN/m and 29.3 mN/m, respectively. In both cases, the highest biosurfactant production was achieved after 24 h of growth. W. anomalus CCMA 0358 demonstrated to be a fast biosurfactant producer (24 h) as compared to other yeast strains previously reported (144-240 h). The produced biosurfactant remained stable at high temperature (121ºC), NaCl concentrations as high as 300 g/L, and pH values between 6 and 12. The crude biosurfactant allowed the recovery of 20% of crude oil from contaminated sand, being a promising candidate for application in bioremediation or in the petroleum industry.The authors acknowledge the Portuguese Foundation for Science and Technology (FCT) for the financial support under the scope of the strategic funding of UID/BIO/04469/2013 unit and COMPETE 2020 (POCI-01-0145-FEDER-006684), and BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020 – Programa Operacional Regional do Norte. The authors also thank the FCT for the financial support under the scope of the Project MultiBiorefinery – Multi‐purpose strategies for broadband agro‐forest and fisheries by‐products valorisation: a step forward for a truly integrated biorefinery (POCI-01-0145-FEDER-016403). E. J. Gudiña was supported by the Post-Doctoral grant CEB-BPD/01/2015/07 from the project UID/BIO/04469/2013, funded by FCT.info:eu-repo/semantics/publishedVersionElsevier 1Universidade do MinhoSouza, K.Gudiña, Eduardo J.Schwan, Rosane F.Rodrigues, L. R.Dias, Disney R.Teixeira, J. A.2018-03-152018-03-15T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/50467engSouza, K.; Gudiña, Eduardo J.; Schwan, Rosane F.; Rodrigues, Lígia R.; Dias, Disney R.; Teixeira, José A., Improvement of biosurfactant production by Wickerhamomyces anomalus CCMA 0358 and its potential application in bioremediation. Journal of Hazardous Materials, 346, 152-158, 20180304-389410.1016/j.jhazmat.2017.12.02129268161http://www.journals.elsevier.com/journal-of-hazardous-materials/info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-07-13T02:03:30Zoai:repositorium.sdum.uminho.pt:1822/50467Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-07-13T02:03:30Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Improvement of biosurfactant production by Wickerhamomyces anomalus CCMA 0358 and its potential application in bioremediation |
title |
Improvement of biosurfactant production by Wickerhamomyces anomalus CCMA 0358 and its potential application in bioremediation |
spellingShingle |
Improvement of biosurfactant production by Wickerhamomyces anomalus CCMA 0358 and its potential application in bioremediation Souza, K. Wickerhamomyces anomalus Biosurfactant Bioreactor Response surface methodology Bioremediation Science & Technology |
title_short |
Improvement of biosurfactant production by Wickerhamomyces anomalus CCMA 0358 and its potential application in bioremediation |
title_full |
Improvement of biosurfactant production by Wickerhamomyces anomalus CCMA 0358 and its potential application in bioremediation |
title_fullStr |
Improvement of biosurfactant production by Wickerhamomyces anomalus CCMA 0358 and its potential application in bioremediation |
title_full_unstemmed |
Improvement of biosurfactant production by Wickerhamomyces anomalus CCMA 0358 and its potential application in bioremediation |
title_sort |
Improvement of biosurfactant production by Wickerhamomyces anomalus CCMA 0358 and its potential application in bioremediation |
author |
Souza, K. |
author_facet |
Souza, K. Gudiña, Eduardo J. Schwan, Rosane F. Rodrigues, L. R. Dias, Disney R. Teixeira, J. A. |
author_role |
author |
author2 |
Gudiña, Eduardo J. Schwan, Rosane F. Rodrigues, L. R. Dias, Disney R. Teixeira, J. A. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Souza, K. Gudiña, Eduardo J. Schwan, Rosane F. Rodrigues, L. R. Dias, Disney R. Teixeira, J. A. |
dc.subject.por.fl_str_mv |
Wickerhamomyces anomalus Biosurfactant Bioreactor Response surface methodology Bioremediation Science & Technology |
topic |
Wickerhamomyces anomalus Biosurfactant Bioreactor Response surface methodology Bioremediation Science & Technology |
description |
In this work, biosurfactant production by Wickerhamomyces anomalus CCMA 0358 was increased through the development of an optimized culture medium using response surface methodology. The optimized culture medium contained yeast extract (4.64 g/L), ammonium sulfate (4.22 g/L), glucose (1.39 g/L) and olive oil (10 g/L). Biosurfactant production using this medium was validated both in flasks and bioreactor, and the surface tension was reduced from 49.0 mN/m up to 31.4 mN/m and 29.3 mN/m, respectively. In both cases, the highest biosurfactant production was achieved after 24 h of growth. W. anomalus CCMA 0358 demonstrated to be a fast biosurfactant producer (24 h) as compared to other yeast strains previously reported (144-240 h). The produced biosurfactant remained stable at high temperature (121ºC), NaCl concentrations as high as 300 g/L, and pH values between 6 and 12. The crude biosurfactant allowed the recovery of 20% of crude oil from contaminated sand, being a promising candidate for application in bioremediation or in the petroleum industry. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-03-15 2018-03-15T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/1822/50467 |
url |
https://hdl.handle.net/1822/50467 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Souza, K.; Gudiña, Eduardo J.; Schwan, Rosane F.; Rodrigues, Lígia R.; Dias, Disney R.; Teixeira, José A., Improvement of biosurfactant production by Wickerhamomyces anomalus CCMA 0358 and its potential application in bioremediation. Journal of Hazardous Materials, 346, 152-158, 2018 0304-3894 10.1016/j.jhazmat.2017.12.021 29268161 http://www.journals.elsevier.com/journal-of-hazardous-materials/ |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier 1 |
publisher.none.fl_str_mv |
Elsevier 1 |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
mluisa.alvim@gmail.com |
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1817544846984871936 |