Evaluation of hematology, general serum biochemistry, bone turnover markers and bone marrow cytology in a glucocorticoid treated ovariectomized sheep model for osteoporosis research

Detalhes bibliográficos
Autor(a) principal: Coelho, Catarina A.
Data de Publicação: 2020
Outros Autores: Bordelo, João P., Camassa, José A., Barros, Vera A., Babo, Pedro Miguel Sousa, Gomes, Manuela E., Reis, R. L., Azevedo, J. T., Requicha, J. F., Faísca, P., Carvalho, P. P., Viegas, C. A., Dias, I. R.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/75582
Resumo: Osteoporosis is a metabolic disorder characterized by a loss of bone mass and structure and increasing the risk of fragility fractures, mostly among postmenopausal women. Sheep is a recognized large animal model for osteoporosis research. An experimental group of ewes (3-4 years old) was subjected to ovariectomy (OVX) and weekly glucocorticoid (GC) application for 24 weeks and compared with a sham control group. Blood and bone marrow parameters were analyzed before and 24 weeks after OVX and GC administration. Osteopenia was confirmed through micro-computed tomography and histomorphometric analysis of L4 vertebra in the study end. A statistically significant increase was observed in mean corpuscular volume, mean cell hemoglobin and monocytes and a decrease in red blood count and eosinophils (p<0.05). Alkaline phosphatase (ALP), gamma-glutamyl transpeptidase, magnesium and α1-globulin increased, and creatinine, albumin, sodium and estradiol decreased (p<0.05). A slight decrease of bone formation markers (bone ALP and osteocalcin) and an increase of bone resorption markers (C-terminal telopeptides of collagen type 1 and tartrate-resistant acid phosphatase) were observed, but without statistical significance. This study aims to contribute to better knowledge of sheep as a model for osteoporosis research and the consequences that a performed induction protocol may impose on organic metabolism.
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spelling Evaluation of hematology, general serum biochemistry, bone turnover markers and bone marrow cytology in a glucocorticoid treated ovariectomized sheep model for osteoporosis researchAnimal model,Blood analysisBone marrow cytologyLumbar vertebral microstructure,OsteoporosisSheepScience & TechnologyOsteoporosis is a metabolic disorder characterized by a loss of bone mass and structure and increasing the risk of fragility fractures, mostly among postmenopausal women. Sheep is a recognized large animal model for osteoporosis research. An experimental group of ewes (3-4 years old) was subjected to ovariectomy (OVX) and weekly glucocorticoid (GC) application for 24 weeks and compared with a sham control group. Blood and bone marrow parameters were analyzed before and 24 weeks after OVX and GC administration. Osteopenia was confirmed through micro-computed tomography and histomorphometric analysis of L4 vertebra in the study end. A statistically significant increase was observed in mean corpuscular volume, mean cell hemoglobin and monocytes and a decrease in red blood count and eosinophils (p<0.05). Alkaline phosphatase (ALP), gamma-glutamyl transpeptidase, magnesium and α1-globulin increased, and creatinine, albumin, sodium and estradiol decreased (p<0.05). A slight decrease of bone formation markers (bone ALP and osteocalcin) and an increase of bone resorption markers (C-terminal telopeptides of collagen type 1 and tartrate-resistant acid phosphatase) were observed, but without statistical significance. This study aims to contribute to better knowledge of sheep as a model for osteoporosis research and the consequences that a performed induction protocol may impose on organic metabolism.This work is funded by national funds through Portuguese Foundation for Science and Technology (FCT) under the projects UlDB/AGR/04033/2020 and UIDB/CVT/00772/2020, and, under the Scientific Employment Stimulus -Institutional Call -CEECINS/00127/2018.Academia Brasileira de CiênciasUniversidade do MinhoCoelho, Catarina A.Bordelo, João P.Camassa, José A.Barros, Vera A.Babo, Pedro Miguel SousaGomes, Manuela E.Reis, R. L.Azevedo, J. T.Requicha, J. F.,Faísca, P.Carvalho, P. P.Viegas, C. A.Dias, I. R.2020-122020-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/75582engCoelho C. A., Bordelo J. P., Camassa J. A., Barros V. A., Babo P. S., Gomes M. E., Reis R. L., Azevedo J. T., Requicha J. F., Faísca P., Carvalho P. P., Viegas C. A., Dias I. R. Evaluation of hematology, general serum biochemistry, bone turnover markers and bone marrow cytology in a glucocorticoid treated ovariectomized sheep model for osteoporosis research, An Acad Bras Cienc, Vol. 92, Issue 4, pp. 2-16, doi:10.1590/0001-3765202020200435, 20201678-269010.1590/0001-376520202020043533295580https://www.scielo.br/j/aabc/a/z44xvqx8BQsH35pk5F9KrhD/?lang=en&format=htmlinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:50:44Zoai:repositorium.sdum.uminho.pt:1822/75582Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:49:29.152093Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Evaluation of hematology, general serum biochemistry, bone turnover markers and bone marrow cytology in a glucocorticoid treated ovariectomized sheep model for osteoporosis research
title Evaluation of hematology, general serum biochemistry, bone turnover markers and bone marrow cytology in a glucocorticoid treated ovariectomized sheep model for osteoporosis research
spellingShingle Evaluation of hematology, general serum biochemistry, bone turnover markers and bone marrow cytology in a glucocorticoid treated ovariectomized sheep model for osteoporosis research
Coelho, Catarina A.
Animal model,
Blood analysis
Bone marrow cytology
Lumbar vertebral microstructure,
Osteoporosis
Sheep
Science & Technology
title_short Evaluation of hematology, general serum biochemistry, bone turnover markers and bone marrow cytology in a glucocorticoid treated ovariectomized sheep model for osteoporosis research
title_full Evaluation of hematology, general serum biochemistry, bone turnover markers and bone marrow cytology in a glucocorticoid treated ovariectomized sheep model for osteoporosis research
title_fullStr Evaluation of hematology, general serum biochemistry, bone turnover markers and bone marrow cytology in a glucocorticoid treated ovariectomized sheep model for osteoporosis research
title_full_unstemmed Evaluation of hematology, general serum biochemistry, bone turnover markers and bone marrow cytology in a glucocorticoid treated ovariectomized sheep model for osteoporosis research
title_sort Evaluation of hematology, general serum biochemistry, bone turnover markers and bone marrow cytology in a glucocorticoid treated ovariectomized sheep model for osteoporosis research
author Coelho, Catarina A.
author_facet Coelho, Catarina A.
Bordelo, João P.
Camassa, José A.
Barros, Vera A.
Babo, Pedro Miguel Sousa
Gomes, Manuela E.
Reis, R. L.
Azevedo, J. T.
Requicha, J. F.,
Faísca, P.
Carvalho, P. P.
Viegas, C. A.
Dias, I. R.
author_role author
author2 Bordelo, João P.
Camassa, José A.
Barros, Vera A.
Babo, Pedro Miguel Sousa
Gomes, Manuela E.
Reis, R. L.
Azevedo, J. T.
Requicha, J. F.,
Faísca, P.
Carvalho, P. P.
Viegas, C. A.
Dias, I. R.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Coelho, Catarina A.
Bordelo, João P.
Camassa, José A.
Barros, Vera A.
Babo, Pedro Miguel Sousa
Gomes, Manuela E.
Reis, R. L.
Azevedo, J. T.
Requicha, J. F.,
Faísca, P.
Carvalho, P. P.
Viegas, C. A.
Dias, I. R.
dc.subject.por.fl_str_mv Animal model,
Blood analysis
Bone marrow cytology
Lumbar vertebral microstructure,
Osteoporosis
Sheep
Science & Technology
topic Animal model,
Blood analysis
Bone marrow cytology
Lumbar vertebral microstructure,
Osteoporosis
Sheep
Science & Technology
description Osteoporosis is a metabolic disorder characterized by a loss of bone mass and structure and increasing the risk of fragility fractures, mostly among postmenopausal women. Sheep is a recognized large animal model for osteoporosis research. An experimental group of ewes (3-4 years old) was subjected to ovariectomy (OVX) and weekly glucocorticoid (GC) application for 24 weeks and compared with a sham control group. Blood and bone marrow parameters were analyzed before and 24 weeks after OVX and GC administration. Osteopenia was confirmed through micro-computed tomography and histomorphometric analysis of L4 vertebra in the study end. A statistically significant increase was observed in mean corpuscular volume, mean cell hemoglobin and monocytes and a decrease in red blood count and eosinophils (p<0.05). Alkaline phosphatase (ALP), gamma-glutamyl transpeptidase, magnesium and α1-globulin increased, and creatinine, albumin, sodium and estradiol decreased (p<0.05). A slight decrease of bone formation markers (bone ALP and osteocalcin) and an increase of bone resorption markers (C-terminal telopeptides of collagen type 1 and tartrate-resistant acid phosphatase) were observed, but without statistical significance. This study aims to contribute to better knowledge of sheep as a model for osteoporosis research and the consequences that a performed induction protocol may impose on organic metabolism.
publishDate 2020
dc.date.none.fl_str_mv 2020-12
2020-12-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/75582
url http://hdl.handle.net/1822/75582
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Coelho C. A., Bordelo J. P., Camassa J. A., Barros V. A., Babo P. S., Gomes M. E., Reis R. L., Azevedo J. T., Requicha J. F., Faísca P., Carvalho P. P., Viegas C. A., Dias I. R. Evaluation of hematology, general serum biochemistry, bone turnover markers and bone marrow cytology in a glucocorticoid treated ovariectomized sheep model for osteoporosis research, An Acad Bras Cienc, Vol. 92, Issue 4, pp. 2-16, doi:10.1590/0001-3765202020200435, 2020
1678-2690
10.1590/0001-3765202020200435
33295580
https://www.scielo.br/j/aabc/a/z44xvqx8BQsH35pk5F9KrhD/?lang=en&format=html
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Academia Brasileira de Ciências
publisher.none.fl_str_mv Academia Brasileira de Ciências
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
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instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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