Adiponectin, Leptin, and IGF-1 are useful diagnostic and stratification biomarkers of NAFLD
| Autor(a) principal: | |
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| Data de Publicação: | 2021 |
| Outros Autores: | , , , , , , , , , , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10451/56538 |
Resumo: | Copyright © 2021 Marques, Afonso, Bierig, Duarte-Ramos, Santos-Laso, Jimenez-Agüero, Eizaguirre, Bujanda, Pareja, Luís, Costa, Machado, Alonso, Arretxe, Alustiza, Krawczyk, Lammert, Tiniakos, Flehmig, Cortez-Pinto, Banales, Castro, Normann and Rodrigues. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
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Adiponectin, Leptin, and IGF-1 are useful diagnostic and stratification biomarkers of NAFLDIGF-1NAFLDAdiponectinCirculating biomarkersFibrosisLeptinLipid metabolismCopyright © 2021 Marques, Afonso, Bierig, Duarte-Ramos, Santos-Laso, Jimenez-Agüero, Eizaguirre, Bujanda, Pareja, Luís, Costa, Machado, Alonso, Arretxe, Alustiza, Krawczyk, Lammert, Tiniakos, Flehmig, Cortez-Pinto, Banales, Castro, Normann and Rodrigues. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.Background: Nonalcoholic fatty liver disease (NAFLD) is the most common chronic liver disease where liver biopsy remains the gold standard for diagnosis. Here we aimed to evaluate the role of circulating adiponectin, leptin, and insulin-like growth factor 1 (IGF-1) levels as non-invasive NAFLD biomarkers and assess their correlation with the metabolome. Materials and Methods: Leptin, adiponectin, and IGF-1 serum levels were measured by ELISA in two independent cohorts of biopsy-proven obese NAFLD patients and healthy-liver controls (discovery: 38 NAFLD, 13 controls; validation: 194 NAFLD, 31 controls) and correlated with clinical data, histology, genetic parameters, and serum metabolomics. Results: In both cohorts, leptin increased in NAFLD vs. controls (discovery: AUROC 0.88; validation: AUROC 0.83; p < 0.0001). The leptin levels were similar between obese and non-obese healthy controls, suggesting that obesity is not a confounding factor. In the discovery cohort, adiponectin was lower in non-alcoholic steatohepatitis (NASH) vs. non-alcoholic fatty liver (AUROC 0.87; p < 0.0001). For the validation cohort, significance was attained for homozygous for PNPLA3 allele c.444C (AUROC 0.63; p < 0.05). Combining adiponectin with specific serum lipids improved the assay performance (AUROC 0.80; p < 0.0001). For the validation cohort, IGF-1 was lower with advanced fibrosis (AUROC 0.67, p < 0.05), but combination with international normalized ratio (INR) and ferritin increased the assay performance (AUROC 0.81; p < 0.01). Conclusion: Serum leptin discriminates NAFLD, and adiponectin combined with specific lipids stratifies NASH. IGF-1, INR, and ferritin distinguish advanced fibrosis.CR was funded by FEDER through the COMPETE program and by national funds through Fundação para a Ciência e a Tecnologia (PTDC/MED-FAR/29097/2017—LISBOA-01-0145-FEDER-029097) and by European Horizon 2020 (H2020-MSCA-RISE-2016-734719). This work was also supported by Fundação para a Ciência e Tecnologia (PD/BD/135467/2017) and Portuguese Association for the Study of Liver/MSD 2017. JB was funded by Spanish Carlos III Health Institute (ISCIII) (PI15/01132, PI18/01075 and Miguel Servet Program CON14/00129 and CPII19/00008), co-financed by Fondo Europeo de Desarrollo Regional (FEDER), Instituto de Salud Carlos III (CIBERehd, Spain), La Caixa Scientific Foundation (HR17-00601), Fundación Científica de la Asociación Española Contra el Cáncer, and European Horizon 2020 (ESCALON project: H2020-SC1-BHC-2018-2020).FrontiersRepositório da Universidade de LisboaMarques, VandaAfonso, MartaBierig, NinaDuarte-Ramos, FilipaSantos-Laso, ÁlvaroJimenez-Agüero, RaulEizaguirre, EmmaBujanda, LuisPareja, Maria J.Luís, RitaCosta, AdíliaMachado, MarianaAlonso, CristinaArretxe, EnaraAlustiza, José M.Krawczyk, MarcinLammert, FrankTiniakos, Dina G.Flehmig, BertramCortez-Pinto, HelenaBanales, Jesus M.Castro, Rui E.Normann, AndreaRodrigues, Cecília M. P.2023-03-03T17:01:17Z20212021-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/56538engFront Med (Lausanne). 2021 Jun 23;8:68325010.3389/fmed.2021.6832502296-858Xinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T17:04:12Zoai:repositorio.ul.pt:10451/56538Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:07:04.739838Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Adiponectin, Leptin, and IGF-1 are useful diagnostic and stratification biomarkers of NAFLD |
| title |
Adiponectin, Leptin, and IGF-1 are useful diagnostic and stratification biomarkers of NAFLD |
| spellingShingle |
Adiponectin, Leptin, and IGF-1 are useful diagnostic and stratification biomarkers of NAFLD Marques, Vanda IGF-1 NAFLD Adiponectin Circulating biomarkers Fibrosis Leptin Lipid metabolism |
| title_short |
Adiponectin, Leptin, and IGF-1 are useful diagnostic and stratification biomarkers of NAFLD |
| title_full |
Adiponectin, Leptin, and IGF-1 are useful diagnostic and stratification biomarkers of NAFLD |
| title_fullStr |
Adiponectin, Leptin, and IGF-1 are useful diagnostic and stratification biomarkers of NAFLD |
| title_full_unstemmed |
Adiponectin, Leptin, and IGF-1 are useful diagnostic and stratification biomarkers of NAFLD |
| title_sort |
Adiponectin, Leptin, and IGF-1 are useful diagnostic and stratification biomarkers of NAFLD |
| author |
Marques, Vanda |
| author_facet |
Marques, Vanda Afonso, Marta Bierig, Nina Duarte-Ramos, Filipa Santos-Laso, Álvaro Jimenez-Agüero, Raul Eizaguirre, Emma Bujanda, Luis Pareja, Maria J. Luís, Rita Costa, Adília Machado, Mariana Alonso, Cristina Arretxe, Enara Alustiza, José M. Krawczyk, Marcin Lammert, Frank Tiniakos, Dina G. Flehmig, Bertram Cortez-Pinto, Helena Banales, Jesus M. Castro, Rui E. Normann, Andrea Rodrigues, Cecília M. P. |
| author_role |
author |
| author2 |
Afonso, Marta Bierig, Nina Duarte-Ramos, Filipa Santos-Laso, Álvaro Jimenez-Agüero, Raul Eizaguirre, Emma Bujanda, Luis Pareja, Maria J. Luís, Rita Costa, Adília Machado, Mariana Alonso, Cristina Arretxe, Enara Alustiza, José M. Krawczyk, Marcin Lammert, Frank Tiniakos, Dina G. Flehmig, Bertram Cortez-Pinto, Helena Banales, Jesus M. Castro, Rui E. Normann, Andrea Rodrigues, Cecília M. P. |
| author2_role |
author author author author author author author author author author author author author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
| dc.contributor.author.fl_str_mv |
Marques, Vanda Afonso, Marta Bierig, Nina Duarte-Ramos, Filipa Santos-Laso, Álvaro Jimenez-Agüero, Raul Eizaguirre, Emma Bujanda, Luis Pareja, Maria J. Luís, Rita Costa, Adília Machado, Mariana Alonso, Cristina Arretxe, Enara Alustiza, José M. Krawczyk, Marcin Lammert, Frank Tiniakos, Dina G. Flehmig, Bertram Cortez-Pinto, Helena Banales, Jesus M. Castro, Rui E. Normann, Andrea Rodrigues, Cecília M. P. |
| dc.subject.por.fl_str_mv |
IGF-1 NAFLD Adiponectin Circulating biomarkers Fibrosis Leptin Lipid metabolism |
| topic |
IGF-1 NAFLD Adiponectin Circulating biomarkers Fibrosis Leptin Lipid metabolism |
| description |
Copyright © 2021 Marques, Afonso, Bierig, Duarte-Ramos, Santos-Laso, Jimenez-Agüero, Eizaguirre, Bujanda, Pareja, Luís, Costa, Machado, Alonso, Arretxe, Alustiza, Krawczyk, Lammert, Tiniakos, Flehmig, Cortez-Pinto, Banales, Castro, Normann and Rodrigues. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
| publishDate |
2021 |
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2021 2021-01-01T00:00:00Z 2023-03-03T17:01:17Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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http://hdl.handle.net/10451/56538 |
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eng |
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eng |
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Front Med (Lausanne). 2021 Jun 23;8:683250 10.3389/fmed.2021.683250 2296-858X |
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Frontiers |
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Frontiers |
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