Antibiotic resistance genes in treated wastewater and in the receiving water bodies: a pan-European survey of urban settings
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.14/28395 |
Resumo: | There is increasing public concern regarding the fate of antibiotic resistance genes (ARGs) during wastewater treatment, their persistence during the treatment process and their potential impacts on the receiving water bodies. In this study, we used quantitative PCR (qPCR) to determine the abundance of nine ARGs and a class 1 integron associated integrase gene in 16 wastewater treatment plant (WWTP) effluents from ten different European countries. In order to assess the impact on the receiving water bodies, gene abundances in the latter were also analysed. Six out of the nine ARGs analysed were detected in all effluent and river water samples. Among the quantified genes, intI1 and sul1 were the most abundant. Our results demonstrate that European WWTP contribute to the enrichment of the resistome in the receiving water bodies with the particular impact being dependent on the effluent load and local hydrological conditions. The ARGs concentrations in WWTP effluents were found to be inversely correlated to the number of implemented biological treatment steps, indicating a possible option for WWTP management. Furthermore, this study has identified blaOXA-58 as a possible resistance gene for future studies investigating the impact of WWTPs on their receiving water. |
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Antibiotic resistance genes in treated wastewater and in the receiving water bodies: a pan-European survey of urban settingsAntibiotic resistance genesWastewaterFreshwaterqPCREuropeThere is increasing public concern regarding the fate of antibiotic resistance genes (ARGs) during wastewater treatment, their persistence during the treatment process and their potential impacts on the receiving water bodies. In this study, we used quantitative PCR (qPCR) to determine the abundance of nine ARGs and a class 1 integron associated integrase gene in 16 wastewater treatment plant (WWTP) effluents from ten different European countries. In order to assess the impact on the receiving water bodies, gene abundances in the latter were also analysed. Six out of the nine ARGs analysed were detected in all effluent and river water samples. Among the quantified genes, intI1 and sul1 were the most abundant. Our results demonstrate that European WWTP contribute to the enrichment of the resistome in the receiving water bodies with the particular impact being dependent on the effluent load and local hydrological conditions. The ARGs concentrations in WWTP effluents were found to be inversely correlated to the number of implemented biological treatment steps, indicating a possible option for WWTP management. Furthermore, this study has identified blaOXA-58 as a possible resistance gene for future studies investigating the impact of WWTPs on their receiving water.ElsevierVeritati - Repositório Institucional da Universidade Católica PortuguesaCacace, DamianoFatta-Kassinos, DespoManaia, Célia M.Cytryn, EddieKreuzinger, NorbertRizzo, LuigiKaraolia, PopiSchwartz, ThomasAlexander, JohannesMerlin, ChristopheGarelick, HemdaSchmitt, HeikeVries, Daisy deSchwermer, Carsten U.Meric, SureyyaOzkal, Can BurakPons, Marie-NoelleKneis, DavidBerendonk, Thomas U.2019-10-17T10:44:11Z20192019-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.14/28395engCacace, D., Fatta-Kassinos, D., Manaia, C. M., Cytryn, E., Kreuzinger, N., Rizzo, L., … Berendonk, T. U. (2019). Antibiotic resistance genes in treated wastewater and in the receiving water bodies: a pan-European survey of urban settings. Water Research, 162, 320–330. https://doi.org/10.1016/j.watres.2019.06.0390043-135410.1016/j.watres.2019.06.0398506846232431288142000479023000031info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-10-03T01:42:02Zoai:repositorio.ucp.pt:10400.14/28395Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:22:47.408Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Antibiotic resistance genes in treated wastewater and in the receiving water bodies: a pan-European survey of urban settings |
title |
Antibiotic resistance genes in treated wastewater and in the receiving water bodies: a pan-European survey of urban settings |
spellingShingle |
Antibiotic resistance genes in treated wastewater and in the receiving water bodies: a pan-European survey of urban settings Cacace, Damiano Antibiotic resistance genes Wastewater Freshwater qPCR Europe |
title_short |
Antibiotic resistance genes in treated wastewater and in the receiving water bodies: a pan-European survey of urban settings |
title_full |
Antibiotic resistance genes in treated wastewater and in the receiving water bodies: a pan-European survey of urban settings |
title_fullStr |
Antibiotic resistance genes in treated wastewater and in the receiving water bodies: a pan-European survey of urban settings |
title_full_unstemmed |
Antibiotic resistance genes in treated wastewater and in the receiving water bodies: a pan-European survey of urban settings |
title_sort |
Antibiotic resistance genes in treated wastewater and in the receiving water bodies: a pan-European survey of urban settings |
author |
Cacace, Damiano |
author_facet |
Cacace, Damiano Fatta-Kassinos, Despo Manaia, Célia M. Cytryn, Eddie Kreuzinger, Norbert Rizzo, Luigi Karaolia, Popi Schwartz, Thomas Alexander, Johannes Merlin, Christophe Garelick, Hemda Schmitt, Heike Vries, Daisy de Schwermer, Carsten U. Meric, Sureyya Ozkal, Can Burak Pons, Marie-Noelle Kneis, David Berendonk, Thomas U. |
author_role |
author |
author2 |
Fatta-Kassinos, Despo Manaia, Célia M. Cytryn, Eddie Kreuzinger, Norbert Rizzo, Luigi Karaolia, Popi Schwartz, Thomas Alexander, Johannes Merlin, Christophe Garelick, Hemda Schmitt, Heike Vries, Daisy de Schwermer, Carsten U. Meric, Sureyya Ozkal, Can Burak Pons, Marie-Noelle Kneis, David Berendonk, Thomas U. |
author2_role |
author author author author author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Veritati - Repositório Institucional da Universidade Católica Portuguesa |
dc.contributor.author.fl_str_mv |
Cacace, Damiano Fatta-Kassinos, Despo Manaia, Célia M. Cytryn, Eddie Kreuzinger, Norbert Rizzo, Luigi Karaolia, Popi Schwartz, Thomas Alexander, Johannes Merlin, Christophe Garelick, Hemda Schmitt, Heike Vries, Daisy de Schwermer, Carsten U. Meric, Sureyya Ozkal, Can Burak Pons, Marie-Noelle Kneis, David Berendonk, Thomas U. |
dc.subject.por.fl_str_mv |
Antibiotic resistance genes Wastewater Freshwater qPCR Europe |
topic |
Antibiotic resistance genes Wastewater Freshwater qPCR Europe |
description |
There is increasing public concern regarding the fate of antibiotic resistance genes (ARGs) during wastewater treatment, their persistence during the treatment process and their potential impacts on the receiving water bodies. In this study, we used quantitative PCR (qPCR) to determine the abundance of nine ARGs and a class 1 integron associated integrase gene in 16 wastewater treatment plant (WWTP) effluents from ten different European countries. In order to assess the impact on the receiving water bodies, gene abundances in the latter were also analysed. Six out of the nine ARGs analysed were detected in all effluent and river water samples. Among the quantified genes, intI1 and sul1 were the most abundant. Our results demonstrate that European WWTP contribute to the enrichment of the resistome in the receiving water bodies with the particular impact being dependent on the effluent load and local hydrological conditions. The ARGs concentrations in WWTP effluents were found to be inversely correlated to the number of implemented biological treatment steps, indicating a possible option for WWTP management. Furthermore, this study has identified blaOXA-58 as a possible resistance gene for future studies investigating the impact of WWTPs on their receiving water. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-17T10:44:11Z 2019 2019-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.14/28395 |
url |
http://hdl.handle.net/10400.14/28395 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Cacace, D., Fatta-Kassinos, D., Manaia, C. M., Cytryn, E., Kreuzinger, N., Rizzo, L., … Berendonk, T. U. (2019). Antibiotic resistance genes in treated wastewater and in the receiving water bodies: a pan-European survey of urban settings. Water Research, 162, 320–330. https://doi.org/10.1016/j.watres.2019.06.039 0043-1354 10.1016/j.watres.2019.06.039 85068462324 31288142 000479023000031 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799131934403592192 |