Combining metagenomics with morphology-based approaches to understand microbiome –meiobenthos interactions
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo de conferência |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10174/31106 |
Resumo: | Sediment microbiome has an essential role in regulating ecosystem functions, not only regulating primary productivity and nutrient cycling but also shaping trophic interactions with higher trophic levels. While the importance of microbiome in terrestrial soil systems is highly recognized, its role in marine aquatic environments remains much less studied. Microbiome can be highly affected by bottom up (abiotic factors) and top down (predation by meiofauna) effects. Understanding the interaction effect between abiotic and biotic factors on microbiome communities will be an essential step for future predictions of ecosystem stability. To address this knowledge gap we studied the interactions among microbiome diversity and nematode communities in highly heterogenous Sado Estuary, SW Portugal. The samples were taken from three contrasting sites with varying sediment characteristics and human impact degrees. From each site, three replicate samples for sediment characterization (total organic matter, granulometry, total C and N), microbiome (sensu lato) and nematode community were taken. Total DNA from sediment was extracted using DNeasy Power Soil kit® (MOBIO, Qiagen) and processed for Illumina MiSeq platform sequencing targeting the V3-V4 region of 16S rRNA gene. Sediment characterization indicated heterogeneity between sites with distinct levels of contamination, which resulted in contrasting microbial communities. All sites showed a high α-biodiversity with predominance of Proteobacteria phylum, particularly Woeseiaceae, Desulfobacteraceae and Rhodobacteraceae families. Beside this heterogeneity in microbiome community, β-diversity was demonstrated to be very high, greatly discriminating among all three sites. Nematode composition and abundance followed a similar pattern as microbiome highlighting influential role of sediment characteristics. Combining abiotic sediment characteristics with metagenomics and morphological identification of higher trophic level communities might certainly contribute to the understanding of complex benthic interactions in marine ecosystems. |
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Combining metagenomics with morphology-based approaches to understand microbiome –meiobenthos interactionsMicrobiome, benthic Nematodes,Metagenomicbenthic nematodesSediment microbiome has an essential role in regulating ecosystem functions, not only regulating primary productivity and nutrient cycling but also shaping trophic interactions with higher trophic levels. While the importance of microbiome in terrestrial soil systems is highly recognized, its role in marine aquatic environments remains much less studied. Microbiome can be highly affected by bottom up (abiotic factors) and top down (predation by meiofauna) effects. Understanding the interaction effect between abiotic and biotic factors on microbiome communities will be an essential step for future predictions of ecosystem stability. To address this knowledge gap we studied the interactions among microbiome diversity and nematode communities in highly heterogenous Sado Estuary, SW Portugal. The samples were taken from three contrasting sites with varying sediment characteristics and human impact degrees. From each site, three replicate samples for sediment characterization (total organic matter, granulometry, total C and N), microbiome (sensu lato) and nematode community were taken. Total DNA from sediment was extracted using DNeasy Power Soil kit® (MOBIO, Qiagen) and processed for Illumina MiSeq platform sequencing targeting the V3-V4 region of 16S rRNA gene. Sediment characterization indicated heterogeneity between sites with distinct levels of contamination, which resulted in contrasting microbial communities. All sites showed a high α-biodiversity with predominance of Proteobacteria phylum, particularly Woeseiaceae, Desulfobacteraceae and Rhodobacteraceae families. Beside this heterogeneity in microbiome community, β-diversity was demonstrated to be very high, greatly discriminating among all three sites. Nematode composition and abundance followed a similar pattern as microbiome highlighting influential role of sediment characteristics. Combining abiotic sediment characteristics with metagenomics and morphological identification of higher trophic level communities might certainly contribute to the understanding of complex benthic interactions in marine ecosystems.ECSA, Elsevier2022-02-22T16:13:39Z2022-02-222021-09-06T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjecthttp://hdl.handle.net/10174/31106http://hdl.handle.net/10174/31106engVieira, S., K. Sroczyńska, K., Martins, M., Costa, M.H., Neves, J., Adão, H., Vicente, C., 2021. Combining metagenomics with morphology-based approaches to understand microbiome –meiobenthos interactions. ECSA&EMECS13, Estuaries and Coastal Seas in the Anthropocene. 6-9 september, Elsevier, Hull, United the KingdomsimnaonaoMARE- Universidade de Évorandndndndndhadao@uevora.ptnd367Vieira, SoraiaSroczynska, KatarzynaMartins, MartaCosta, M.H.Neves, JoanaAdão, HelenaVicente, Claudiainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T19:30:20Zoai:dspace.uevora.pt:10174/31106Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:20:22.571Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Combining metagenomics with morphology-based approaches to understand microbiome –meiobenthos interactions |
title |
Combining metagenomics with morphology-based approaches to understand microbiome –meiobenthos interactions |
spellingShingle |
Combining metagenomics with morphology-based approaches to understand microbiome –meiobenthos interactions Vieira, Soraia Microbiome, benthic Nematodes, Metagenomic benthic nematodes |
title_short |
Combining metagenomics with morphology-based approaches to understand microbiome –meiobenthos interactions |
title_full |
Combining metagenomics with morphology-based approaches to understand microbiome –meiobenthos interactions |
title_fullStr |
Combining metagenomics with morphology-based approaches to understand microbiome –meiobenthos interactions |
title_full_unstemmed |
Combining metagenomics with morphology-based approaches to understand microbiome –meiobenthos interactions |
title_sort |
Combining metagenomics with morphology-based approaches to understand microbiome –meiobenthos interactions |
author |
Vieira, Soraia |
author_facet |
Vieira, Soraia Sroczynska, Katarzyna Martins, Marta Costa, M.H. Neves, Joana Adão, Helena Vicente, Claudia |
author_role |
author |
author2 |
Sroczynska, Katarzyna Martins, Marta Costa, M.H. Neves, Joana Adão, Helena Vicente, Claudia |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Vieira, Soraia Sroczynska, Katarzyna Martins, Marta Costa, M.H. Neves, Joana Adão, Helena Vicente, Claudia |
dc.subject.por.fl_str_mv |
Microbiome, benthic Nematodes, Metagenomic benthic nematodes |
topic |
Microbiome, benthic Nematodes, Metagenomic benthic nematodes |
description |
Sediment microbiome has an essential role in regulating ecosystem functions, not only regulating primary productivity and nutrient cycling but also shaping trophic interactions with higher trophic levels. While the importance of microbiome in terrestrial soil systems is highly recognized, its role in marine aquatic environments remains much less studied. Microbiome can be highly affected by bottom up (abiotic factors) and top down (predation by meiofauna) effects. Understanding the interaction effect between abiotic and biotic factors on microbiome communities will be an essential step for future predictions of ecosystem stability. To address this knowledge gap we studied the interactions among microbiome diversity and nematode communities in highly heterogenous Sado Estuary, SW Portugal. The samples were taken from three contrasting sites with varying sediment characteristics and human impact degrees. From each site, three replicate samples for sediment characterization (total organic matter, granulometry, total C and N), microbiome (sensu lato) and nematode community were taken. Total DNA from sediment was extracted using DNeasy Power Soil kit® (MOBIO, Qiagen) and processed for Illumina MiSeq platform sequencing targeting the V3-V4 region of 16S rRNA gene. Sediment characterization indicated heterogeneity between sites with distinct levels of contamination, which resulted in contrasting microbial communities. All sites showed a high α-biodiversity with predominance of Proteobacteria phylum, particularly Woeseiaceae, Desulfobacteraceae and Rhodobacteraceae families. Beside this heterogeneity in microbiome community, β-diversity was demonstrated to be very high, greatly discriminating among all three sites. Nematode composition and abundance followed a similar pattern as microbiome highlighting influential role of sediment characteristics. Combining abiotic sediment characteristics with metagenomics and morphological identification of higher trophic level communities might certainly contribute to the understanding of complex benthic interactions in marine ecosystems. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-09-06T00:00:00Z 2022-02-22T16:13:39Z 2022-02-22 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/conferenceObject |
format |
conferenceObject |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10174/31106 http://hdl.handle.net/10174/31106 |
url |
http://hdl.handle.net/10174/31106 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Vieira, S., K. Sroczyńska, K., Martins, M., Costa, M.H., Neves, J., Adão, H., Vicente, C., 2021. Combining metagenomics with morphology-based approaches to understand microbiome –meiobenthos interactions. ECSA&EMECS13, Estuaries and Coastal Seas in the Anthropocene. 6-9 september, Elsevier, Hull, United the Kingdom sim nao nao MARE- Universidade de Évora nd nd nd nd nd hadao@uevora.pt nd 367 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
ECSA, Elsevier |
publisher.none.fl_str_mv |
ECSA, Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799136685651394560 |