Gardnerella vaginalis outcompetes 29 other bacterial species isolated from BV patients in an in vitro biofilm formation model

Detalhes bibliográficos
Autor(a) principal: Alves, Patrícia Maria
Data de Publicação: 2014
Outros Autores: Castro, J., Sousa, Cármen, Cereija, Tatiana Barros Reis, Cerca, Nuno
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/32708
Resumo: Despite the worldwide prevalence of bacterial vaginosis (BV), its etiology is still unknown. Although BV has been associated with the presence of biofilm, the ability of BV-associated bacteria to form biofilms is still largely unknown. Here, we isolated 30 BV-associated species and characterized their virulence, using an in vitro biofilm formation model. Our data suggests that Gardnerella vaginalis had the highest virulence potential, as defined by higher initial adhesion and cytotoxicity of epithelial cells, as well as the greater propensity to form a biofilm. Interestingly, we also demonstrated that most of the BV-associated bacteria had a tendency to grow as biofilms.
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spelling Gardnerella vaginalis outcompetes 29 other bacterial species isolated from BV patients in an in vitro biofilm formation modelBacterial vaginosisMulti-species biofilmVirulence determinationScience & TechnologyDespite the worldwide prevalence of bacterial vaginosis (BV), its etiology is still unknown. Although BV has been associated with the presence of biofilm, the ability of BV-associated bacteria to form biofilms is still largely unknown. Here, we isolated 30 BV-associated species and characterized their virulence, using an in vitro biofilm formation model. Our data suggests that Gardnerella vaginalis had the highest virulence potential, as defined by higher initial adhesion and cytotoxicity of epithelial cells, as well as the greater propensity to form a biofilm. Interestingly, we also demonstrated that most of the BV-associated bacteria had a tendency to grow as biofilms.This work was supported by the European Union (FEDER/COMPETE funds) and the Fundacao para a Ciencia e a Tecnologia (reference FCOMP-01-0124-FEDER-008991 [PTDC/BIA-MIC/098228/2008] and RECI/EBB-EBI/0179/2012 [FCOMP-01-0124-FEDER-027462]).University of Chicago PressUniversidade do MinhoAlves, Patrícia MariaCastro, J.Sousa, CármenCereija, Tatiana Barros ReisCerca, Nuno2014-03-052014-03-05T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/32708engAlves, P.; Castro, Joana; Sousa, Cármen; Cereija, T. B.; Cerca, Nuno, Gardnerella vaginalis outcompetes 29 other bacterial species isolated from BV patients in an in vitro biofilm formation model. Journal of Infectious Diseases, 210(4), 593-596, 20140022-18991537-661310.1093/infdis/jiu13124596283info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:21:00Zoai:repositorium.sdum.uminho.pt:1822/32708Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:14:10.762031Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Gardnerella vaginalis outcompetes 29 other bacterial species isolated from BV patients in an in vitro biofilm formation model
title Gardnerella vaginalis outcompetes 29 other bacterial species isolated from BV patients in an in vitro biofilm formation model
spellingShingle Gardnerella vaginalis outcompetes 29 other bacterial species isolated from BV patients in an in vitro biofilm formation model
Alves, Patrícia Maria
Bacterial vaginosis
Multi-species biofilm
Virulence determination
Science & Technology
title_short Gardnerella vaginalis outcompetes 29 other bacterial species isolated from BV patients in an in vitro biofilm formation model
title_full Gardnerella vaginalis outcompetes 29 other bacterial species isolated from BV patients in an in vitro biofilm formation model
title_fullStr Gardnerella vaginalis outcompetes 29 other bacterial species isolated from BV patients in an in vitro biofilm formation model
title_full_unstemmed Gardnerella vaginalis outcompetes 29 other bacterial species isolated from BV patients in an in vitro biofilm formation model
title_sort Gardnerella vaginalis outcompetes 29 other bacterial species isolated from BV patients in an in vitro biofilm formation model
author Alves, Patrícia Maria
author_facet Alves, Patrícia Maria
Castro, J.
Sousa, Cármen
Cereija, Tatiana Barros Reis
Cerca, Nuno
author_role author
author2 Castro, J.
Sousa, Cármen
Cereija, Tatiana Barros Reis
Cerca, Nuno
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Alves, Patrícia Maria
Castro, J.
Sousa, Cármen
Cereija, Tatiana Barros Reis
Cerca, Nuno
dc.subject.por.fl_str_mv Bacterial vaginosis
Multi-species biofilm
Virulence determination
Science & Technology
topic Bacterial vaginosis
Multi-species biofilm
Virulence determination
Science & Technology
description Despite the worldwide prevalence of bacterial vaginosis (BV), its etiology is still unknown. Although BV has been associated with the presence of biofilm, the ability of BV-associated bacteria to form biofilms is still largely unknown. Here, we isolated 30 BV-associated species and characterized their virulence, using an in vitro biofilm formation model. Our data suggests that Gardnerella vaginalis had the highest virulence potential, as defined by higher initial adhesion and cytotoxicity of epithelial cells, as well as the greater propensity to form a biofilm. Interestingly, we also demonstrated that most of the BV-associated bacteria had a tendency to grow as biofilms.
publishDate 2014
dc.date.none.fl_str_mv 2014-03-05
2014-03-05T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/32708
url http://hdl.handle.net/1822/32708
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Alves, P.; Castro, Joana; Sousa, Cármen; Cereija, T. B.; Cerca, Nuno, Gardnerella vaginalis outcompetes 29 other bacterial species isolated from BV patients in an in vitro biofilm formation model. Journal of Infectious Diseases, 210(4), 593-596, 2014
0022-1899
1537-6613
10.1093/infdis/jiu131
24596283
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dc.publisher.none.fl_str_mv University of Chicago Press
publisher.none.fl_str_mv University of Chicago Press
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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