The reductive glycine pathway allows autotrophic growth of Desulfovibrio desulfuricans

Detalhes bibliográficos
Autor(a) principal: Sánchez-Andrea, Irene
Data de Publicação: 2020
Outros Autores: Guedes, Iame Alves, Hornung, Bastian, Boeren, Sjef, Lawson, Christopher E., Sousa, Diana Zita Machado, Bar-Even, Arren, Claassens, Nico J., Stams, Alfons Johannes Maria
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/67463
Resumo: Supplementary informationis available for this paper athttps://doi.org/10.1038/s41467-020-18906-7
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spelling The reductive glycine pathway allows autotrophic growth of Desulfovibrio desulfuricansScience & TechnologySupplementary informationis available for this paper athttps://doi.org/10.1038/s41467-020-18906-7Six CO2 fixation pathways are known to operate in photoautotrophic and chemoautotrophic microorganisms. Here, we describe chemolithoautotrophic growth of the sulphate-reducing bacterium Desulfovibrio desulfuricans (strain G11) with hydrogen and sulphate as energy substrates. Genomic, transcriptomic, proteomic and metabolomic analyses reveal that D. desulfuricans assimilates CO2 via the reductive glycine pathway, a seventh CO2 fixation pathway. In this pathway, CO2 is first reduced to formate, which is reduced and condensed with a second CO2 to generate glycine. Glycine is further reduced in D. desulfuricans by glycine reductase to acetyl-P, and then to acetyl-CoA, which is condensed with another CO2 to form pyruvate. Ammonia is involved in the operation of the pathway, which is reflected in the dependence of the autotrophic growth rate on the ammonia concentration. Our study demonstrates microbial autotrophic growth fully supported by this highly ATP-efficient CO2 fixation pathway.We acknowledge Änne-Michaelis and William Newell for assistance with the LC-MS forthe metabolomics experiments and Daniel Amador-Noguez for access to the LC-MS usedfor13C intracellular metabolomic analysis. We thank Ines Cardoso Pereira and John vander Oost for critically reading the manuscript. This research was funded by the Neth-erlands Organisation for Scientific Research (NWO) through SIAM Gravitation Grant024.002.002 and the Innovation Program Microbiology (WUR), NJC acknowledgesfunding from NWO through a Rubicon Grant (019.163LW.035) and a Veni Grant(VI.Veni.192.156).info:eu-repo/semantics/publishedVersionSpringer NatureUniversidade do MinhoSánchez-Andrea, IreneGuedes, Iame AlvesHornung, BastianBoeren, SjefLawson, Christopher E.Sousa, Diana Zita MachadoBar-Even, ArrenClaassens, Nico J.Stams, Alfons Johannes Maria20202020-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/67463engSánchez-Andrea, Irene; Guedes, Iame Alves; Hornung, Bastian; Boeren, Sjef; Lawson, Christopher E.; Sousa, Diana Z.; Bar-Even, Arren; Claassens, Nico J.; Stams, A. J. M., The reductive glycine pathway allows autotrophic growth of Desulfovibrio desulfuricans. Nature Communications, 11(5090), 20202041-172310.1038/s41467-020-18906-733037220https://www.nature.com/articles/s41467-020-18906-7info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-11T05:31:01Zoai:repositorium.sdum.uminho.pt:1822/67463Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-11T05:31:01Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv The reductive glycine pathway allows autotrophic growth of Desulfovibrio desulfuricans
title The reductive glycine pathway allows autotrophic growth of Desulfovibrio desulfuricans
spellingShingle The reductive glycine pathway allows autotrophic growth of Desulfovibrio desulfuricans
Sánchez-Andrea, Irene
Science & Technology
title_short The reductive glycine pathway allows autotrophic growth of Desulfovibrio desulfuricans
title_full The reductive glycine pathway allows autotrophic growth of Desulfovibrio desulfuricans
title_fullStr The reductive glycine pathway allows autotrophic growth of Desulfovibrio desulfuricans
title_full_unstemmed The reductive glycine pathway allows autotrophic growth of Desulfovibrio desulfuricans
title_sort The reductive glycine pathway allows autotrophic growth of Desulfovibrio desulfuricans
author Sánchez-Andrea, Irene
author_facet Sánchez-Andrea, Irene
Guedes, Iame Alves
Hornung, Bastian
Boeren, Sjef
Lawson, Christopher E.
Sousa, Diana Zita Machado
Bar-Even, Arren
Claassens, Nico J.
Stams, Alfons Johannes Maria
author_role author
author2 Guedes, Iame Alves
Hornung, Bastian
Boeren, Sjef
Lawson, Christopher E.
Sousa, Diana Zita Machado
Bar-Even, Arren
Claassens, Nico J.
Stams, Alfons Johannes Maria
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Sánchez-Andrea, Irene
Guedes, Iame Alves
Hornung, Bastian
Boeren, Sjef
Lawson, Christopher E.
Sousa, Diana Zita Machado
Bar-Even, Arren
Claassens, Nico J.
Stams, Alfons Johannes Maria
dc.subject.por.fl_str_mv Science & Technology
topic Science & Technology
description Supplementary informationis available for this paper athttps://doi.org/10.1038/s41467-020-18906-7
publishDate 2020
dc.date.none.fl_str_mv 2020
2020-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/67463
url http://hdl.handle.net/1822/67463
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Sánchez-Andrea, Irene; Guedes, Iame Alves; Hornung, Bastian; Boeren, Sjef; Lawson, Christopher E.; Sousa, Diana Z.; Bar-Even, Arren; Claassens, Nico J.; Stams, A. J. M., The reductive glycine pathway allows autotrophic growth of Desulfovibrio desulfuricans. Nature Communications, 11(5090), 2020
2041-1723
10.1038/s41467-020-18906-7
33037220
https://www.nature.com/articles/s41467-020-18906-7
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer Nature
publisher.none.fl_str_mv Springer Nature
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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