Adsorption of peroxidase from Raphanus sativus L onto alginateguar gum matrix: Kinetic, equilibrium and thermodynamic analysis

Detalhes bibliográficos
Autor(a) principal: Ana Catarina S. L. Silva
Data de Publicação: 2016
Outros Autores: Valetti, Nadia Woitovich, Brassesco, María E., Teixeira, J. A., Picó, Guillermo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/47683
Resumo: This work explores the kinetics, equilibrium and thermodynamics of peroxidase adsorption onto spherical guar gumalginate matrices. The effect of contact time, solution pH, initial protein concentration and temperature was studied in batch experiments. The results show that peroxidase adsorption increased with rising contact time and initial enzyme concentration, and was higher at pH 4.0. The kinetic processes can be predicted by both the pseudo-first-order rate kinetics and the pseudo-second-order rate kinetics. Equilibrium adsorption data were analyzed with different isotherm models. The experimental data fitted to the Freundlich model in agreement with the low energy activation, demonstrating the presence of a high physical and unspecific interaction between the enzyme and the matrix.
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spelling Adsorption of peroxidase from Raphanus sativus L onto alginateguar gum matrix: Kinetic, equilibrium and thermodynamic analysisAlginateguar gumpolyelectrolytesperoxidaseadsorptionisothermsScience & TechnologyThis work explores the kinetics, equilibrium and thermodynamics of peroxidase adsorption onto spherical guar gumalginate matrices. The effect of contact time, solution pH, initial protein concentration and temperature was studied in batch experiments. The results show that peroxidase adsorption increased with rising contact time and initial enzyme concentration, and was higher at pH 4.0. The kinetic processes can be predicted by both the pseudo-first-order rate kinetics and the pseudo-second-order rate kinetics. Equilibrium adsorption data were analyzed with different isotherm models. The experimental data fitted to the Freundlich model in agreement with the low energy activation, demonstrating the presence of a high physical and unspecific interaction between the enzyme and the matrix.The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: FonCyT, Projects PICT 2013–271 – Argentina Innovator 2020 and Biotechnologies to Valorize the regional food Biodiversity in Latin America – Marie Curie Actions – IRSES Project number 611493 – European Community.info:eu-repo/semantics/publishedVersionMulti-Science PublishingUniversidade do MinhoAna Catarina S. L. SilvaValetti, Nadia WoitovichBrassesco, María E.Teixeira, J. A.Picó, Guillermo20162016-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/47683engAna Catarina S. L. Silva; Valetti, Nadia Woitovich; Brassesco, María E.; Teixeira, José A.; Picó, Guillermo, Adsorption of peroxidase from Raphanus sativus L onto alginateguar gum matrix: Kinetic, equilibrium and thermodynamic analysis. Adsorption Science & Technology, 34(6), 388-402, 2016026361742048-403810.1177/0263617416659287http://journals.sagepub.com/home/adtinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:08:07Zoai:repositorium.sdum.uminho.pt:1822/47683Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:59:15.016075Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Adsorption of peroxidase from Raphanus sativus L onto alginateguar gum matrix: Kinetic, equilibrium and thermodynamic analysis
title Adsorption of peroxidase from Raphanus sativus L onto alginateguar gum matrix: Kinetic, equilibrium and thermodynamic analysis
spellingShingle Adsorption of peroxidase from Raphanus sativus L onto alginateguar gum matrix: Kinetic, equilibrium and thermodynamic analysis
Ana Catarina S. L. Silva
Alginate
guar gum
polyelectrolytes
peroxidase
adsorption
isotherms
Science & Technology
title_short Adsorption of peroxidase from Raphanus sativus L onto alginateguar gum matrix: Kinetic, equilibrium and thermodynamic analysis
title_full Adsorption of peroxidase from Raphanus sativus L onto alginateguar gum matrix: Kinetic, equilibrium and thermodynamic analysis
title_fullStr Adsorption of peroxidase from Raphanus sativus L onto alginateguar gum matrix: Kinetic, equilibrium and thermodynamic analysis
title_full_unstemmed Adsorption of peroxidase from Raphanus sativus L onto alginateguar gum matrix: Kinetic, equilibrium and thermodynamic analysis
title_sort Adsorption of peroxidase from Raphanus sativus L onto alginateguar gum matrix: Kinetic, equilibrium and thermodynamic analysis
author Ana Catarina S. L. Silva
author_facet Ana Catarina S. L. Silva
Valetti, Nadia Woitovich
Brassesco, María E.
Teixeira, J. A.
Picó, Guillermo
author_role author
author2 Valetti, Nadia Woitovich
Brassesco, María E.
Teixeira, J. A.
Picó, Guillermo
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Ana Catarina S. L. Silva
Valetti, Nadia Woitovich
Brassesco, María E.
Teixeira, J. A.
Picó, Guillermo
dc.subject.por.fl_str_mv Alginate
guar gum
polyelectrolytes
peroxidase
adsorption
isotherms
Science & Technology
topic Alginate
guar gum
polyelectrolytes
peroxidase
adsorption
isotherms
Science & Technology
description This work explores the kinetics, equilibrium and thermodynamics of peroxidase adsorption onto spherical guar gumalginate matrices. The effect of contact time, solution pH, initial protein concentration and temperature was studied in batch experiments. The results show that peroxidase adsorption increased with rising contact time and initial enzyme concentration, and was higher at pH 4.0. The kinetic processes can be predicted by both the pseudo-first-order rate kinetics and the pseudo-second-order rate kinetics. Equilibrium adsorption data were analyzed with different isotherm models. The experimental data fitted to the Freundlich model in agreement with the low energy activation, demonstrating the presence of a high physical and unspecific interaction between the enzyme and the matrix.
publishDate 2016
dc.date.none.fl_str_mv 2016
2016-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/47683
url http://hdl.handle.net/1822/47683
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Ana Catarina S. L. Silva; Valetti, Nadia Woitovich; Brassesco, María E.; Teixeira, José A.; Picó, Guillermo, Adsorption of peroxidase from Raphanus sativus L onto alginateguar gum matrix: Kinetic, equilibrium and thermodynamic analysis. Adsorption Science & Technology, 34(6), 388-402, 2016
02636174
2048-4038
10.1177/0263617416659287
http://journals.sagepub.com/home/adt
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Multi-Science Publishing
publisher.none.fl_str_mv Multi-Science Publishing
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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