IgE to epitopes of Ara h 2 enhance the diagnostic accuracy of Ara h 2‐specific IgE
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2020 |
| Outros Autores: | , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10451/50032 |
Resumo: | © 2020 The Authors. Allergy published by European Academy of Allergy and Clinical Immunology and John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
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IgE to epitopes of Ara h 2 enhance the diagnostic accuracy of Ara h 2‐specific IgEAra h 2DiagnosisEpitopesFood allergy peanut allergy© 2020 The Authors. Allergy published by European Academy of Allergy and Clinical Immunology and John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.Background: Understanding the discrepancy between IgE sensitization and allergic reactions to peanut could facilitate diagnosis and lead to novel means of treating peanut allergy. Objective: To identify differences in IgE and IgG4 binding to peanut peptides between peanut-allergic (PA) and peanut-sensitized but tolerant (PS) children. Methods: PA (n = 56), PS (n = 42) and nonsensitized nonallergic (NA, n = 10) patients were studied. Synthetic overlapping 15-mer peptides of peanut allergens (Ara h 1-11) were spotted onto microarray slides, and patients' samples were tested for IgE and IgG4 binding using immunofluorescence. IgE and IgG4 levels to selected peptides were quantified using ImmunoCAP. Diagnostic model comparisons were performed using likelihood-ratio tests between each specified nominal logistic regression models. Results: Seven peptides on Ara h 1, Ara h 2, and Ara h 3 were bound more by IgE of PA compared to PS patients on the microarray. IgE binding to one peptide on Ara h 5 and IgG4 binding to one Ara h 9 peptide were greater in PS than in PA patients. Using ImmunoCAP, IgE to the Ara h 2 peptides enhanced the diagnostic accuracy of Ara h 2-specific IgE. Ratios of IgG4/IgE to 4 out of the 7 peptides were higher in PS than in PA subjects. Conclusions: Ara h 2 peptide-specific IgE added diagnostic value to Ara h 2-specific IgE. Ability of peptide-specific IgG4 to surmount their IgE counterpart seems to be important in established peanut tolerance.This work was supported by the Medical Research Council (MRC Clinical Research Training Fellowship G090218, MRC Centenary Early Career Award and MRC Clinician Scientist Fellowship MR/M008517/1, all awarded to A. F. Santos), the Department of Health via the National Institute for Health Research (NIHR) comprehensive Biomedical Research Centre award to Guy's & St Thomas' NHS Foundation Trust in partnership with King's College London and King's College Hospital NHS Foundation Trust; the US Department of Agriculture, Agricultural Research Service (USDA-ARS6054-43440-046-00D; USDA-NIFA) and the National Peanut Board (GRANT12229460).John Wiley & Sons, Inc.Repositório da Universidade de LisboaSantos, Alexandra F.Barbosa-Morais, NunoHurlburt, Barry K.Ramaswamy, SnehaHemmings, OliverKwok, MatthewO’Rourke, ColinBahnson, Henry T.Cheng, HsiaopoJames, LouisaGould, Hannah J.Sutton, Brian J.Maleki, Soheila J.Lack, Gideon2021-10-27T14:08:55Z20202020-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/50032engAllergy. 2020 Sep;75(9):2309-23180105-453810.1111/all.143011398-9995info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:54:03Zoai:repositorio.ul.pt:10451/50032Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:01:33.200161Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
IgE to epitopes of Ara h 2 enhance the diagnostic accuracy of Ara h 2‐specific IgE |
| title |
IgE to epitopes of Ara h 2 enhance the diagnostic accuracy of Ara h 2‐specific IgE |
| spellingShingle |
IgE to epitopes of Ara h 2 enhance the diagnostic accuracy of Ara h 2‐specific IgE Santos, Alexandra F. Ara h 2 Diagnosis Epitopes Food allergy peanut allergy |
| title_short |
IgE to epitopes of Ara h 2 enhance the diagnostic accuracy of Ara h 2‐specific IgE |
| title_full |
IgE to epitopes of Ara h 2 enhance the diagnostic accuracy of Ara h 2‐specific IgE |
| title_fullStr |
IgE to epitopes of Ara h 2 enhance the diagnostic accuracy of Ara h 2‐specific IgE |
| title_full_unstemmed |
IgE to epitopes of Ara h 2 enhance the diagnostic accuracy of Ara h 2‐specific IgE |
| title_sort |
IgE to epitopes of Ara h 2 enhance the diagnostic accuracy of Ara h 2‐specific IgE |
| author |
Santos, Alexandra F. |
| author_facet |
Santos, Alexandra F. Barbosa-Morais, Nuno Hurlburt, Barry K. Ramaswamy, Sneha Hemmings, Oliver Kwok, Matthew O’Rourke, Colin Bahnson, Henry T. Cheng, Hsiaopo James, Louisa Gould, Hannah J. Sutton, Brian J. Maleki, Soheila J. Lack, Gideon |
| author_role |
author |
| author2 |
Barbosa-Morais, Nuno Hurlburt, Barry K. Ramaswamy, Sneha Hemmings, Oliver Kwok, Matthew O’Rourke, Colin Bahnson, Henry T. Cheng, Hsiaopo James, Louisa Gould, Hannah J. Sutton, Brian J. Maleki, Soheila J. Lack, Gideon |
| author2_role |
author author author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
| dc.contributor.author.fl_str_mv |
Santos, Alexandra F. Barbosa-Morais, Nuno Hurlburt, Barry K. Ramaswamy, Sneha Hemmings, Oliver Kwok, Matthew O’Rourke, Colin Bahnson, Henry T. Cheng, Hsiaopo James, Louisa Gould, Hannah J. Sutton, Brian J. Maleki, Soheila J. Lack, Gideon |
| dc.subject.por.fl_str_mv |
Ara h 2 Diagnosis Epitopes Food allergy peanut allergy |
| topic |
Ara h 2 Diagnosis Epitopes Food allergy peanut allergy |
| description |
© 2020 The Authors. Allergy published by European Academy of Allergy and Clinical Immunology and John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
| publishDate |
2020 |
| dc.date.none.fl_str_mv |
2020 2020-01-01T00:00:00Z 2021-10-27T14:08:55Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
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article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10451/50032 |
| url |
http://hdl.handle.net/10451/50032 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Allergy. 2020 Sep;75(9):2309-2318 0105-4538 10.1111/all.14301 1398-9995 |
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info:eu-repo/semantics/openAccess |
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openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
John Wiley & Sons, Inc. |
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John Wiley & Sons, Inc. |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799134563730980864 |