Multi-mycotoxin analysis using dried blood spots and dried serum spots
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
DOI: | 10.1007/s00216-017-0279-9 |
Texto Completo: | https://doi.org/10.1007/s00216-017-0279-9 |
Resumo: | In this study, a rapid multi-mycotoxin approach was developed for biomonitoring and quantification of 27 important mycotoxins and mycotoxin metabolites in human blood samples. HPLC-MS/MS detection was used for the analysis of dried serum spots (DSS) and dried blood spots (DBS). Detection of aflatoxins (AFB1, AFB2, AFG1, AFG2, AFM1), trichothecenes (deoxynivalenol, DON; DON-3-glucoronic acid, DON-3-GlcA; T-2; HT-2; and HT-2-4-GlcA), fumonisin B1 (FB1), ochratoxins (OTA and its thermal degradation product 2’R-OTA; OTα; 10-hydroxychratoxin A, 10-OH-OTA), citrinin (CIT and its urinary metabolite dihydrocitrinone, DH-CIT), zearalenone and zearalanone (ZEN, ZAN), altenuene (ALT), alternariols (AOH; alternariol monomethyl ether, AME), enniatins (EnA, EnA1, EnB, EnB1) and beauvericin (Bea) was validated for two matrices, serum (DSS), and whole blood (DBS). HPLC-MS/MS analysis showed signal suppression as well as signal enhancement due to matrix effects. However, for most analytes LOQs in the lower pg/mL range and excellent recovery rate were achieved using matrix-matched calibration. Besides validation of the method, the analyte stability in DBS and DSS was also investigated. Stability is a main issue for some analytes when the dried samples are stored under common conditions at room temperature. Nevertheless, the developed method was applied to DBS samples of a German cohort (n = 50). Besides positive findings of OTA and 2’R-OTA, all samples were positive for EnB. This methodical study establishes a validated multi-mycotoxin approach for the detection of 27 mycotoxins and metabolites in dried blood/serum spots based on a fast sample preparation followed by sensitive HPLC-MS/MS analysis. [Figure not available: see fulltext.] |
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7160 |
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Multi-mycotoxin analysis using dried blood spots and dried serum spotsBiomonitoringDried blood spotDried serum spotHPLC-MS/MSMass spectrometryMycotoxinAnalytical ChemistryBiochemistryIn this study, a rapid multi-mycotoxin approach was developed for biomonitoring and quantification of 27 important mycotoxins and mycotoxin metabolites in human blood samples. HPLC-MS/MS detection was used for the analysis of dried serum spots (DSS) and dried blood spots (DBS). Detection of aflatoxins (AFB1, AFB2, AFG1, AFG2, AFM1), trichothecenes (deoxynivalenol, DON; DON-3-glucoronic acid, DON-3-GlcA; T-2; HT-2; and HT-2-4-GlcA), fumonisin B1 (FB1), ochratoxins (OTA and its thermal degradation product 2’R-OTA; OTα; 10-hydroxychratoxin A, 10-OH-OTA), citrinin (CIT and its urinary metabolite dihydrocitrinone, DH-CIT), zearalenone and zearalanone (ZEN, ZAN), altenuene (ALT), alternariols (AOH; alternariol monomethyl ether, AME), enniatins (EnA, EnA1, EnB, EnB1) and beauvericin (Bea) was validated for two matrices, serum (DSS), and whole blood (DBS). HPLC-MS/MS analysis showed signal suppression as well as signal enhancement due to matrix effects. However, for most analytes LOQs in the lower pg/mL range and excellent recovery rate were achieved using matrix-matched calibration. Besides validation of the method, the analyte stability in DBS and DSS was also investigated. Stability is a main issue for some analytes when the dried samples are stored under common conditions at room temperature. Nevertheless, the developed method was applied to DBS samples of a German cohort (n = 50). Besides positive findings of OTA and 2’R-OTA, all samples were positive for EnB. This methodical study establishes a validated multi-mycotoxin approach for the detection of 27 mycotoxins and metabolites in dried blood/serum spots based on a fast sample preparation followed by sensitive HPLC-MS/MS analysis. [Figure not available: see fulltext.]Centro de Investigação em Saúde Pública (CISP/PHRC)RUNOsteresch, BerndViegas , SusanaCramer, BenediktHumpf, Hans Ulrich2018-02-14T23:10:09Z2017-05-012017-05-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article14application/pdfhttps://doi.org/10.1007/s00216-017-0279-9eng1618-2642PURE: 2987803http://www.scopus.com/inward/record.url?scp=85015230050&partnerID=8YFLogxKhttps://doi.org/10.1007/s00216-017-0279-9info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-22T17:30:41Zoai:run.unl.pt:10362/30504Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-22T17:30:41Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Multi-mycotoxin analysis using dried blood spots and dried serum spots |
title |
Multi-mycotoxin analysis using dried blood spots and dried serum spots |
spellingShingle |
Multi-mycotoxin analysis using dried blood spots and dried serum spots Multi-mycotoxin analysis using dried blood spots and dried serum spots Osteresch, Bernd Biomonitoring Dried blood spot Dried serum spot HPLC-MS/MS Mass spectrometry Mycotoxin Analytical Chemistry Biochemistry Osteresch, Bernd Biomonitoring Dried blood spot Dried serum spot HPLC-MS/MS Mass spectrometry Mycotoxin Analytical Chemistry Biochemistry |
title_short |
Multi-mycotoxin analysis using dried blood spots and dried serum spots |
title_full |
Multi-mycotoxin analysis using dried blood spots and dried serum spots |
title_fullStr |
Multi-mycotoxin analysis using dried blood spots and dried serum spots Multi-mycotoxin analysis using dried blood spots and dried serum spots |
title_full_unstemmed |
Multi-mycotoxin analysis using dried blood spots and dried serum spots Multi-mycotoxin analysis using dried blood spots and dried serum spots |
title_sort |
Multi-mycotoxin analysis using dried blood spots and dried serum spots |
author |
Osteresch, Bernd |
author_facet |
Osteresch, Bernd Osteresch, Bernd Viegas , Susana Cramer, Benedikt Humpf, Hans Ulrich Viegas , Susana Cramer, Benedikt Humpf, Hans Ulrich |
author_role |
author |
author2 |
Viegas , Susana Cramer, Benedikt Humpf, Hans Ulrich |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Centro de Investigação em Saúde Pública (CISP/PHRC) RUN |
dc.contributor.author.fl_str_mv |
Osteresch, Bernd Viegas , Susana Cramer, Benedikt Humpf, Hans Ulrich |
dc.subject.por.fl_str_mv |
Biomonitoring Dried blood spot Dried serum spot HPLC-MS/MS Mass spectrometry Mycotoxin Analytical Chemistry Biochemistry |
topic |
Biomonitoring Dried blood spot Dried serum spot HPLC-MS/MS Mass spectrometry Mycotoxin Analytical Chemistry Biochemistry |
description |
In this study, a rapid multi-mycotoxin approach was developed for biomonitoring and quantification of 27 important mycotoxins and mycotoxin metabolites in human blood samples. HPLC-MS/MS detection was used for the analysis of dried serum spots (DSS) and dried blood spots (DBS). Detection of aflatoxins (AFB1, AFB2, AFG1, AFG2, AFM1), trichothecenes (deoxynivalenol, DON; DON-3-glucoronic acid, DON-3-GlcA; T-2; HT-2; and HT-2-4-GlcA), fumonisin B1 (FB1), ochratoxins (OTA and its thermal degradation product 2’R-OTA; OTα; 10-hydroxychratoxin A, 10-OH-OTA), citrinin (CIT and its urinary metabolite dihydrocitrinone, DH-CIT), zearalenone and zearalanone (ZEN, ZAN), altenuene (ALT), alternariols (AOH; alternariol monomethyl ether, AME), enniatins (EnA, EnA1, EnB, EnB1) and beauvericin (Bea) was validated for two matrices, serum (DSS), and whole blood (DBS). HPLC-MS/MS analysis showed signal suppression as well as signal enhancement due to matrix effects. However, for most analytes LOQs in the lower pg/mL range and excellent recovery rate were achieved using matrix-matched calibration. Besides validation of the method, the analyte stability in DBS and DSS was also investigated. Stability is a main issue for some analytes when the dried samples are stored under common conditions at room temperature. Nevertheless, the developed method was applied to DBS samples of a German cohort (n = 50). Besides positive findings of OTA and 2’R-OTA, all samples were positive for EnB. This methodical study establishes a validated multi-mycotoxin approach for the detection of 27 mycotoxins and metabolites in dried blood/serum spots based on a fast sample preparation followed by sensitive HPLC-MS/MS analysis. [Figure not available: see fulltext.] |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-05-01 2017-05-01T00:00:00Z 2018-02-14T23:10:09Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://doi.org/10.1007/s00216-017-0279-9 |
url |
https://doi.org/10.1007/s00216-017-0279-9 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1618-2642 PURE: 2987803 http://www.scopus.com/inward/record.url?scp=85015230050&partnerID=8YFLogxK https://doi.org/10.1007/s00216-017-0279-9 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
14 application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
mluisa.alvim@gmail.com |
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1822181943962238976 |
dc.identifier.doi.none.fl_str_mv |
10.1007/s00216-017-0279-9 |