Aerocyte specification and lung adaptation to breathing is dependent on alternative splicing changes

Detalhes bibliográficos
Autor(a) principal: Fidalgo, Marta F.
Data de Publicação: 2022
Outros Autores: Fonseca, Catarina G., Caldas, Paulo, Raposo, Alexandre A. S. F., Balboni, Tania, Henao-Mišíková, Lenka, Grosso, Ana R., Vasconcelos, Francisca F., Franco, Cláudio A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/150363
Resumo: Funding Information: ariana Ferreira, Marie Bordone, Nuno Agostinho and Nuno Barbosa Morais (Disease Transcriptomics Lab, iMM) for input on bioinformatics analysis. Pedro Papotto, Karine Serre (Immuno-Biology & Immuno-Oncology Lab, iMM), Idálio Viegas (Biology of Parasitism Lab, iMM), Isabel Alcobia (Institute of Histology and Developmental Biology, FMUL), Debanjan Mukherjee, Vanessa Zuzarte-Luís (Biology & Physiology of Malaria, iMM), and Mahak Singhal (Vascular Oncology and Metastasis Division, DKFZ), for input on experimental protocols. Luís Oliveira (Cell Architecture Lab, iMM) for input on microscopy image analysis. Flow Cytometry, Bioimaging, Rodent and Comparative Pathology Units at iMM for technical support. All members of the Vascular Morphogenesis lab at iMM for discussions, helpful input and for carefully reviewing the manuscript. Nuno Barbosa Morais for carefully reading this manuscript and providing helpful and critical feedback. This work was supported by the Fondation Leducq (17CVD03). T Balboni was supported by a PhD fellowship from the doctoral program “Oncology, Hematology and Pathology - 30th Cycle” funded by University of Bologna, Italy. AASF Raposo was supported by FCT and Fundo Europeu de Desenvolvimento Regional (FEDER) PAC-PRECISE-LISBOA-01-0145-FEDER-016394. Publisher Copyright: © 2022 Fidalgo et al.
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spelling Aerocyte specification and lung adaptation to breathing is dependent on alternative splicing changesEcologyBiochemistry, Genetics and Molecular Biology (miscellaneous)Plant ScienceHealth, Toxicology and MutagenesisFunding Information: ariana Ferreira, Marie Bordone, Nuno Agostinho and Nuno Barbosa Morais (Disease Transcriptomics Lab, iMM) for input on bioinformatics analysis. Pedro Papotto, Karine Serre (Immuno-Biology & Immuno-Oncology Lab, iMM), Idálio Viegas (Biology of Parasitism Lab, iMM), Isabel Alcobia (Institute of Histology and Developmental Biology, FMUL), Debanjan Mukherjee, Vanessa Zuzarte-Luís (Biology & Physiology of Malaria, iMM), and Mahak Singhal (Vascular Oncology and Metastasis Division, DKFZ), for input on experimental protocols. Luís Oliveira (Cell Architecture Lab, iMM) for input on microscopy image analysis. Flow Cytometry, Bioimaging, Rodent and Comparative Pathology Units at iMM for technical support. All members of the Vascular Morphogenesis lab at iMM for discussions, helpful input and for carefully reviewing the manuscript. Nuno Barbosa Morais for carefully reading this manuscript and providing helpful and critical feedback. This work was supported by the Fondation Leducq (17CVD03). T Balboni was supported by a PhD fellowship from the doctoral program “Oncology, Hematology and Pathology - 30th Cycle” funded by University of Bologna, Italy. AASF Raposo was supported by FCT and Fundo Europeu de Desenvolvimento Regional (FEDER) PAC-PRECISE-LISBOA-01-0145-FEDER-016394. Publisher Copyright: © 2022 Fidalgo et al.Adaptation to breathing is a critical step in lung function and it is crucial for organismal survival. Alveoli are the lung gas exchange units and their development, from late embryonic to early postnatal stages, requires feedbacks between multiple cell types. However, how the crosstalk between the alveolar cell types is modulated to anticipate lung adaptation to breathing is still unclear. Here, we uncovered a synchronous alternative splicing switch in multiple genes in the developing mouse lungs at the transition to birth, and we identified hnRNP A1, Cpeb4, and Elavl2/ HuB as putative splicing regulators of this transition. Notably, we found that Vegfa switches from the Vegfa 164 isoform to the longer Vegfa 188 isoform exclusively in lung alveolar epithelial AT1 cells. Functional analysis revealed that VEGFA 188 (and not VEGFA 164) drives the specification of Car4-positive aerocytes, a subtype of alveolar endothelial cells specialized in gas exchanges. Our results reveal that the cell type–specific regulation of Vegfa alternative splicing just before birth modulates the epithelial-endothelial crosstalk in the developing alveoli to promote lung adaptation to breathing.UCIBIO - Applied Molecular Biosciences UnitDCV - Departamento de Ciências da VidaRUNFidalgo, Marta F.Fonseca, Catarina G.Caldas, PauloRaposo, Alexandre A. S. F.Balboni, TaniaHenao-Mišíková, LenkaGrosso, Ana R.Vasconcelos, Francisca F.Franco, Cláudio A.2023-03-10T22:54:41Z2022-122022-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article25application/pdfhttp://hdl.handle.net/10362/150363engPURE: 55291958https://doi.org/10.26508/lsa.202201554info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:32:21Zoai:run.unl.pt:10362/150363Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:54:04.596892Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Aerocyte specification and lung adaptation to breathing is dependent on alternative splicing changes
title Aerocyte specification and lung adaptation to breathing is dependent on alternative splicing changes
spellingShingle Aerocyte specification and lung adaptation to breathing is dependent on alternative splicing changes
Fidalgo, Marta F.
Ecology
Biochemistry, Genetics and Molecular Biology (miscellaneous)
Plant Science
Health, Toxicology and Mutagenesis
title_short Aerocyte specification and lung adaptation to breathing is dependent on alternative splicing changes
title_full Aerocyte specification and lung adaptation to breathing is dependent on alternative splicing changes
title_fullStr Aerocyte specification and lung adaptation to breathing is dependent on alternative splicing changes
title_full_unstemmed Aerocyte specification and lung adaptation to breathing is dependent on alternative splicing changes
title_sort Aerocyte specification and lung adaptation to breathing is dependent on alternative splicing changes
author Fidalgo, Marta F.
author_facet Fidalgo, Marta F.
Fonseca, Catarina G.
Caldas, Paulo
Raposo, Alexandre A. S. F.
Balboni, Tania
Henao-Mišíková, Lenka
Grosso, Ana R.
Vasconcelos, Francisca F.
Franco, Cláudio A.
author_role author
author2 Fonseca, Catarina G.
Caldas, Paulo
Raposo, Alexandre A. S. F.
Balboni, Tania
Henao-Mišíková, Lenka
Grosso, Ana R.
Vasconcelos, Francisca F.
Franco, Cláudio A.
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv UCIBIO - Applied Molecular Biosciences Unit
DCV - Departamento de Ciências da Vida
RUN
dc.contributor.author.fl_str_mv Fidalgo, Marta F.
Fonseca, Catarina G.
Caldas, Paulo
Raposo, Alexandre A. S. F.
Balboni, Tania
Henao-Mišíková, Lenka
Grosso, Ana R.
Vasconcelos, Francisca F.
Franco, Cláudio A.
dc.subject.por.fl_str_mv Ecology
Biochemistry, Genetics and Molecular Biology (miscellaneous)
Plant Science
Health, Toxicology and Mutagenesis
topic Ecology
Biochemistry, Genetics and Molecular Biology (miscellaneous)
Plant Science
Health, Toxicology and Mutagenesis
description Funding Information: ariana Ferreira, Marie Bordone, Nuno Agostinho and Nuno Barbosa Morais (Disease Transcriptomics Lab, iMM) for input on bioinformatics analysis. Pedro Papotto, Karine Serre (Immuno-Biology & Immuno-Oncology Lab, iMM), Idálio Viegas (Biology of Parasitism Lab, iMM), Isabel Alcobia (Institute of Histology and Developmental Biology, FMUL), Debanjan Mukherjee, Vanessa Zuzarte-Luís (Biology & Physiology of Malaria, iMM), and Mahak Singhal (Vascular Oncology and Metastasis Division, DKFZ), for input on experimental protocols. Luís Oliveira (Cell Architecture Lab, iMM) for input on microscopy image analysis. Flow Cytometry, Bioimaging, Rodent and Comparative Pathology Units at iMM for technical support. All members of the Vascular Morphogenesis lab at iMM for discussions, helpful input and for carefully reviewing the manuscript. Nuno Barbosa Morais for carefully reading this manuscript and providing helpful and critical feedback. This work was supported by the Fondation Leducq (17CVD03). T Balboni was supported by a PhD fellowship from the doctoral program “Oncology, Hematology and Pathology - 30th Cycle” funded by University of Bologna, Italy. AASF Raposo was supported by FCT and Fundo Europeu de Desenvolvimento Regional (FEDER) PAC-PRECISE-LISBOA-01-0145-FEDER-016394. Publisher Copyright: © 2022 Fidalgo et al.
publishDate 2022
dc.date.none.fl_str_mv 2022-12
2022-12-01T00:00:00Z
2023-03-10T22:54:41Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/150363
url http://hdl.handle.net/10362/150363
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv PURE: 55291958
https://doi.org/10.26508/lsa.202201554
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
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dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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