Stress response markers in the blood of São Tomé green sea turtles (Chelonia mydas) and their relation with accumulated metal levels

Detalhes bibliográficos
Autor(a) principal: Morão, Inês F.C.
Data de Publicação: 2022
Outros Autores: Lemos, Marco F.L., Félix, Rafael, Vieira, Sara, Barata, Carlos, Novais, Sara C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.8/7555
Resumo: Acknowledgements : This study had the support of Fundação para a Ciência e a Tecnologia (FCT) through the Strategic Project UID/MAR/04292/2020 and the grants awarded to Inês Morão (PD/BD/150562/2019) and Rafael Félix (SFRH/BD/139763/2018). The project was also partially funded by the Integrated Programme of SR&TD “SmartBioR” (reference Centro-01-0145-FEDER-000018) cofunded by Centro (2020) program, Portugal 2020, European Union, through the European Regional Development Fund. Sara Novais is funded by national funds (OE), through FCT – Fundação para a Ciência e a Tecnologia, I.P., in the scope of the framework contract foreseen in the numbers 4, 5 and 6 of the article 23, of the Decree-Law 57/2016, of August 29, changed by Law 57/2017, of July 19. The authors which to acknowledge the support of the Clear Reef Social Fund to grant awarded to Inês Morão. We are thankful to all the members of NGO Programa Tatô and research assistants (biologists Maria Branco and Yedda Oliveira) working at Jalé Beach and to Aurélio Evaristo and João Evaristo that helped us in the field under challenging conditions. We are also thankful to Marta Carmona for the help given in the treatment of the samples for cytology and micronucleus analysis.
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spelling Stress response markers in the blood of São Tomé green sea turtles (Chelonia mydas) and their relation with accumulated metal levelsBiomarkersGene expressionMarine pollutionMarine turtlesOxidative stressAcknowledgements : This study had the support of Fundação para a Ciência e a Tecnologia (FCT) through the Strategic Project UID/MAR/04292/2020 and the grants awarded to Inês Morão (PD/BD/150562/2019) and Rafael Félix (SFRH/BD/139763/2018). The project was also partially funded by the Integrated Programme of SR&TD “SmartBioR” (reference Centro-01-0145-FEDER-000018) cofunded by Centro (2020) program, Portugal 2020, European Union, through the European Regional Development Fund. Sara Novais is funded by national funds (OE), through FCT – Fundação para a Ciência e a Tecnologia, I.P., in the scope of the framework contract foreseen in the numbers 4, 5 and 6 of the article 23, of the Decree-Law 57/2016, of August 29, changed by Law 57/2017, of July 19. The authors which to acknowledge the support of the Clear Reef Social Fund to grant awarded to Inês Morão. We are thankful to all the members of NGO Programa Tatô and research assistants (biologists Maria Branco and Yedda Oliveira) working at Jalé Beach and to Aurélio Evaristo and João Evaristo that helped us in the field under challenging conditions. We are also thankful to Marta Carmona for the help given in the treatment of the samples for cytology and micronucleus analysis.Metals are persistent worldwide being harmful for diverse organisms and having complex and combined effects with other contaminants in the environment. Sea turtles accumulate these contaminants being considered good bioindicator species for marine pollution. However, very little is known on how this is affecting these charismatic animals. Sâo Tomé and Príncipe archipelago harbours important green sea turtle (Chelonia mydas) nesting and feeding grounds. The main goal of this study was to determine metal and metalloid accumulation in the blood of females C. mydas nesting in São Tomé Island, and evaluate the possible impacts of this contamination by addressing molecular stress responses. Gene expression analysis was performed in blood targeting genes involved in detoxification/sequestration and metal transport (mt, mtf and fer), and in antioxidant and oxidative stress responses (cat, sod, gr, tdx, txrd, selp and gclc). Micronuclei analysis in blood was also addressed as a biomarker of genotoxicity. Present results showed significant correlations between different gene expressions with the metals evaluated. The best GLM models and significant relationships were found for mt expression, for which 78% of the variability was attributed to metal levels (Al, Cu, Fe, Hg, Pb and Zn), followed by micronuclei count (65% - Cr, Cu, Fe, Hg, Mn and Zn), tdx expression (52% - Cd, Fe, Mn, Pb and Se), and cat expression (52% - As, Fe, Se and Cd x Hg). Overall, this study demonstrates that these green sea turtles are trying to adapt to the oxidative stress and damage produced by metals through the increased expression of antioxidants and other protectors, which raises concerns about the impacts on these endangered organisms’ fitness. Furthermore, promising biomarker candidates associated to metal stress were identified in this species that may be used in future biomonitoring studies using C. mydas’ blood, allowing for a temporal follow-up of the organisms.ElsevierIC-OnlineMorão, Inês F.C.Lemos, Marco F.L.Félix, RafaelVieira, SaraBarata, CarlosNovais, Sara C.2022-08-17T14:07:16Z20222022-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.8/7555engInês F.C. Morão, Marco F.L. Lemos, Rafael Félix, Sara Vieira, Carlos Barata, Sara C. Novais, Stress response markers in the blood of São Tomé green sea turtles (Chelonia mydas) and their relation with accumulated metal levels, Environmental Pollution, Volume 293, 2022, 118490, ISSN 0269-7491, https://doi.org/10.1016/j.envpol.2021.1184900269-749110.1016/j.envpol.2021.118490metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-17T15:55:26Zoai:iconline.ipleiria.pt:10400.8/7555Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:50:29.418437Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Stress response markers in the blood of São Tomé green sea turtles (Chelonia mydas) and their relation with accumulated metal levels
title Stress response markers in the blood of São Tomé green sea turtles (Chelonia mydas) and their relation with accumulated metal levels
spellingShingle Stress response markers in the blood of São Tomé green sea turtles (Chelonia mydas) and their relation with accumulated metal levels
Morão, Inês F.C.
Biomarkers
Gene expression
Marine pollution
Marine turtles
Oxidative stress
title_short Stress response markers in the blood of São Tomé green sea turtles (Chelonia mydas) and their relation with accumulated metal levels
title_full Stress response markers in the blood of São Tomé green sea turtles (Chelonia mydas) and their relation with accumulated metal levels
title_fullStr Stress response markers in the blood of São Tomé green sea turtles (Chelonia mydas) and their relation with accumulated metal levels
title_full_unstemmed Stress response markers in the blood of São Tomé green sea turtles (Chelonia mydas) and their relation with accumulated metal levels
title_sort Stress response markers in the blood of São Tomé green sea turtles (Chelonia mydas) and their relation with accumulated metal levels
author Morão, Inês F.C.
author_facet Morão, Inês F.C.
Lemos, Marco F.L.
Félix, Rafael
Vieira, Sara
Barata, Carlos
Novais, Sara C.
author_role author
author2 Lemos, Marco F.L.
Félix, Rafael
Vieira, Sara
Barata, Carlos
Novais, Sara C.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv IC-Online
dc.contributor.author.fl_str_mv Morão, Inês F.C.
Lemos, Marco F.L.
Félix, Rafael
Vieira, Sara
Barata, Carlos
Novais, Sara C.
dc.subject.por.fl_str_mv Biomarkers
Gene expression
Marine pollution
Marine turtles
Oxidative stress
topic Biomarkers
Gene expression
Marine pollution
Marine turtles
Oxidative stress
description Acknowledgements : This study had the support of Fundação para a Ciência e a Tecnologia (FCT) through the Strategic Project UID/MAR/04292/2020 and the grants awarded to Inês Morão (PD/BD/150562/2019) and Rafael Félix (SFRH/BD/139763/2018). The project was also partially funded by the Integrated Programme of SR&TD “SmartBioR” (reference Centro-01-0145-FEDER-000018) cofunded by Centro (2020) program, Portugal 2020, European Union, through the European Regional Development Fund. Sara Novais is funded by national funds (OE), through FCT – Fundação para a Ciência e a Tecnologia, I.P., in the scope of the framework contract foreseen in the numbers 4, 5 and 6 of the article 23, of the Decree-Law 57/2016, of August 29, changed by Law 57/2017, of July 19. The authors which to acknowledge the support of the Clear Reef Social Fund to grant awarded to Inês Morão. We are thankful to all the members of NGO Programa Tatô and research assistants (biologists Maria Branco and Yedda Oliveira) working at Jalé Beach and to Aurélio Evaristo and João Evaristo that helped us in the field under challenging conditions. We are also thankful to Marta Carmona for the help given in the treatment of the samples for cytology and micronucleus analysis.
publishDate 2022
dc.date.none.fl_str_mv 2022-08-17T14:07:16Z
2022
2022-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.8/7555
url http://hdl.handle.net/10400.8/7555
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Inês F.C. Morão, Marco F.L. Lemos, Rafael Félix, Sara Vieira, Carlos Barata, Sara C. Novais, Stress response markers in the blood of São Tomé green sea turtles (Chelonia mydas) and their relation with accumulated metal levels, Environmental Pollution, Volume 293, 2022, 118490, ISSN 0269-7491, https://doi.org/10.1016/j.envpol.2021.118490
0269-7491
10.1016/j.envpol.2021.118490
dc.rights.driver.fl_str_mv metadata only access
info:eu-repo/semantics/openAccess
rights_invalid_str_mv metadata only access
eu_rights_str_mv openAccess
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dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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