Antioxidant, mineralogenic and osteogenic activities of Spartina alterniflora and Salicornia fragilis extracts rich in polyphenols

Detalhes bibliográficos
Autor(a) principal: Roberto, Vania Palma
Data de Publicação: 2021
Outros Autores: Surget, Gwladys, Le Lann, Klervi, Mira, Sara, Tarasco, Marco, Guérard, Fabienne, Poupart, Nathalie, Laizé, Vincent, Stiger-Pouvreau, Valérie, Cancela, M. Leonor
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.1/17110
Resumo: Osteoporosis is an aging-related disease and a worldwide health issue. Current therapeutics have failed to reduce the prevalence of osteoporosis in the human population, thus the discovery of compounds with bone anabolic properties that could be the basis of next generation drugs is a priority. Marine plants contain a wide range of bioactive compounds and the presence of osteoactive phytochemicals was investigated in two halophytes collected in Brittany (France): the invasive Spartina alterniflora and the native Salicornia fragilis. Two semi-purified fractions, prepared through liquid-liquid extraction, were assessed for phenolic and flavonoid contents, and for the presence of antioxidant, mineralogenic and osteogenic bioactivities. Ethyl acetate fraction (EAF) wasrich in phenolic compounds and exhibited the highest antioxidant activity. While S. fragilis EAF only triggered a weak proliferative effect in vitro, S. alterniflora EAF potently induced extracellular matrix mineralization (7-fold at 250µg/mL). A strong osteogenic effect was also observed in vivo using zebrafish operculum assay (2.5-fold at 10µg/mL in 9-dpf larvae). Results indicate that polyphenol rich EAF of S. alterniflora has both antioxidant and bone anabolic activities. As an invasive species, this marine plant may represent a sustainable source of molecules for therapeutic applications in bone disorders.
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spelling Antioxidant, mineralogenic and osteogenic activities of Spartina alterniflora and Salicornia fragilis extracts rich in polyphenolsAtividades antioxidantes, mineralogênicas e osteogênicas de Spartina alterniflora e Salicornia fragilis extratos ricos em PolifenóisAntioxidantPhenolic compoundsMineralizationOsteogenesisOsteoporosisMarine plantsOsteoporosis is an aging-related disease and a worldwide health issue. Current therapeutics have failed to reduce the prevalence of osteoporosis in the human population, thus the discovery of compounds with bone anabolic properties that could be the basis of next generation drugs is a priority. Marine plants contain a wide range of bioactive compounds and the presence of osteoactive phytochemicals was investigated in two halophytes collected in Brittany (France): the invasive Spartina alterniflora and the native Salicornia fragilis. Two semi-purified fractions, prepared through liquid-liquid extraction, were assessed for phenolic and flavonoid contents, and for the presence of antioxidant, mineralogenic and osteogenic bioactivities. Ethyl acetate fraction (EAF) wasrich in phenolic compounds and exhibited the highest antioxidant activity. While S. fragilis EAF only triggered a weak proliferative effect in vitro, S. alterniflora EAF potently induced extracellular matrix mineralization (7-fold at 250µg/mL). A strong osteogenic effect was also observed in vivo using zebrafish operculum assay (2.5-fold at 10µg/mL in 9-dpf larvae). Results indicate that polyphenol rich EAF of S. alterniflora has both antioxidant and bone anabolic activities. As an invasive species, this marine plant may represent a sustainable source of molecules for therapeutic applications in bone disorders.Frontiers MediaSapientiaRoberto, Vania PalmaSurget, GwladysLe Lann, KlerviMira, SaraTarasco, MarcoGuérard, FabiennePoupart, NathalieLaizé, VincentStiger-Pouvreau, ValérieCancela, M. Leonor2021-09-14T16:26:44Z2021-082021-08-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.1/17110eng10.3389/fnut.2021.719438info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-11-29T10:57:54Zoai:sapientia.ualg.pt:10400.1/17110Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-11-29T10:57:54Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Antioxidant, mineralogenic and osteogenic activities of Spartina alterniflora and Salicornia fragilis extracts rich in polyphenols
Atividades antioxidantes, mineralogênicas e osteogênicas de Spartina alterniflora e Salicornia fragilis extratos ricos em Polifenóis
title Antioxidant, mineralogenic and osteogenic activities of Spartina alterniflora and Salicornia fragilis extracts rich in polyphenols
spellingShingle Antioxidant, mineralogenic and osteogenic activities of Spartina alterniflora and Salicornia fragilis extracts rich in polyphenols
Roberto, Vania Palma
Antioxidant
Phenolic compounds
Mineralization
Osteogenesis
Osteoporosis
Marine plants
title_short Antioxidant, mineralogenic and osteogenic activities of Spartina alterniflora and Salicornia fragilis extracts rich in polyphenols
title_full Antioxidant, mineralogenic and osteogenic activities of Spartina alterniflora and Salicornia fragilis extracts rich in polyphenols
title_fullStr Antioxidant, mineralogenic and osteogenic activities of Spartina alterniflora and Salicornia fragilis extracts rich in polyphenols
title_full_unstemmed Antioxidant, mineralogenic and osteogenic activities of Spartina alterniflora and Salicornia fragilis extracts rich in polyphenols
title_sort Antioxidant, mineralogenic and osteogenic activities of Spartina alterniflora and Salicornia fragilis extracts rich in polyphenols
author Roberto, Vania Palma
author_facet Roberto, Vania Palma
Surget, Gwladys
Le Lann, Klervi
Mira, Sara
Tarasco, Marco
Guérard, Fabienne
Poupart, Nathalie
Laizé, Vincent
Stiger-Pouvreau, Valérie
Cancela, M. Leonor
author_role author
author2 Surget, Gwladys
Le Lann, Klervi
Mira, Sara
Tarasco, Marco
Guérard, Fabienne
Poupart, Nathalie
Laizé, Vincent
Stiger-Pouvreau, Valérie
Cancela, M. Leonor
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Sapientia
dc.contributor.author.fl_str_mv Roberto, Vania Palma
Surget, Gwladys
Le Lann, Klervi
Mira, Sara
Tarasco, Marco
Guérard, Fabienne
Poupart, Nathalie
Laizé, Vincent
Stiger-Pouvreau, Valérie
Cancela, M. Leonor
dc.subject.por.fl_str_mv Antioxidant
Phenolic compounds
Mineralization
Osteogenesis
Osteoporosis
Marine plants
topic Antioxidant
Phenolic compounds
Mineralization
Osteogenesis
Osteoporosis
Marine plants
description Osteoporosis is an aging-related disease and a worldwide health issue. Current therapeutics have failed to reduce the prevalence of osteoporosis in the human population, thus the discovery of compounds with bone anabolic properties that could be the basis of next generation drugs is a priority. Marine plants contain a wide range of bioactive compounds and the presence of osteoactive phytochemicals was investigated in two halophytes collected in Brittany (France): the invasive Spartina alterniflora and the native Salicornia fragilis. Two semi-purified fractions, prepared through liquid-liquid extraction, were assessed for phenolic and flavonoid contents, and for the presence of antioxidant, mineralogenic and osteogenic bioactivities. Ethyl acetate fraction (EAF) wasrich in phenolic compounds and exhibited the highest antioxidant activity. While S. fragilis EAF only triggered a weak proliferative effect in vitro, S. alterniflora EAF potently induced extracellular matrix mineralization (7-fold at 250µg/mL). A strong osteogenic effect was also observed in vivo using zebrafish operculum assay (2.5-fold at 10µg/mL in 9-dpf larvae). Results indicate that polyphenol rich EAF of S. alterniflora has both antioxidant and bone anabolic activities. As an invasive species, this marine plant may represent a sustainable source of molecules for therapeutic applications in bone disorders.
publishDate 2021
dc.date.none.fl_str_mv 2021-09-14T16:26:44Z
2021-08
2021-08-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.1/17110
url http://hdl.handle.net/10400.1/17110
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.3389/fnut.2021.719438
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eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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