EzrA contributes to the regulation of cell size in Staphylococcus aureus

Detalhes bibliográficos
Autor(a) principal: Jorge, A.M.
Data de Publicação: 2011
Outros Autores: Hoiczyk, E., Gomes, João Paulo, Pinho, M.G.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.18/632
Resumo: EzrA is a negative regulator of FtsZ in Bacillus subtilis, involved in the coordination between cell growth and cell division and in the control of the cell elongation-division cycle. We have now studied the role of the Staphylococcus aureus homologue of the B. subtilis EzrA protein and shown that it is not essential for cell viability. EzrA conditional and null mutants have an overall increase of the average cell size, compared to wild type strains. In the larger ezrA mutant S. aureus cells, cell division protein FtsZ and the cell wall synthesizing Penicillin Binding Proteins (PBPs) are not properly localized. This suggests that there may be a maximum cell diameter that allows formation of a Z-ring capable of recruiting the other components of the divisome and of driving cytokinesis. We propose that the major role of EzrA in S. aureus is in cell size homeostasis.
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spelling EzrA contributes to the regulation of cell size in Staphylococcus aureusInfecções RespiratóriasStaphylococcus AureusEzrA is a negative regulator of FtsZ in Bacillus subtilis, involved in the coordination between cell growth and cell division and in the control of the cell elongation-division cycle. We have now studied the role of the Staphylococcus aureus homologue of the B. subtilis EzrA protein and shown that it is not essential for cell viability. EzrA conditional and null mutants have an overall increase of the average cell size, compared to wild type strains. In the larger ezrA mutant S. aureus cells, cell division protein FtsZ and the cell wall synthesizing Penicillin Binding Proteins (PBPs) are not properly localized. This suggests that there may be a maximum cell diameter that allows formation of a Z-ring capable of recruiting the other components of the divisome and of driving cytokinesis. We propose that the major role of EzrA in S. aureus is in cell size homeostasis.Public Library of ScienceRepositório Científico do Instituto Nacional de SaúdeJorge, A.M.Hoiczyk, E.Gomes, João PauloPinho, M.G.2012-02-24T17:44:33Z20112011-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/632engPLoS One. 2011;6(11):e27542. Epub 2011 Nov 141932-6203doi:10.1371/journal.pone.0027542info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:38:23Zoai:repositorio.insa.pt:10400.18/632Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:35:59.472794Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv EzrA contributes to the regulation of cell size in Staphylococcus aureus
title EzrA contributes to the regulation of cell size in Staphylococcus aureus
spellingShingle EzrA contributes to the regulation of cell size in Staphylococcus aureus
Jorge, A.M.
Infecções Respiratórias
Staphylococcus Aureus
title_short EzrA contributes to the regulation of cell size in Staphylococcus aureus
title_full EzrA contributes to the regulation of cell size in Staphylococcus aureus
title_fullStr EzrA contributes to the regulation of cell size in Staphylococcus aureus
title_full_unstemmed EzrA contributes to the regulation of cell size in Staphylococcus aureus
title_sort EzrA contributes to the regulation of cell size in Staphylococcus aureus
author Jorge, A.M.
author_facet Jorge, A.M.
Hoiczyk, E.
Gomes, João Paulo
Pinho, M.G.
author_role author
author2 Hoiczyk, E.
Gomes, João Paulo
Pinho, M.G.
author2_role author
author
author
dc.contributor.none.fl_str_mv Repositório Científico do Instituto Nacional de Saúde
dc.contributor.author.fl_str_mv Jorge, A.M.
Hoiczyk, E.
Gomes, João Paulo
Pinho, M.G.
dc.subject.por.fl_str_mv Infecções Respiratórias
Staphylococcus Aureus
topic Infecções Respiratórias
Staphylococcus Aureus
description EzrA is a negative regulator of FtsZ in Bacillus subtilis, involved in the coordination between cell growth and cell division and in the control of the cell elongation-division cycle. We have now studied the role of the Staphylococcus aureus homologue of the B. subtilis EzrA protein and shown that it is not essential for cell viability. EzrA conditional and null mutants have an overall increase of the average cell size, compared to wild type strains. In the larger ezrA mutant S. aureus cells, cell division protein FtsZ and the cell wall synthesizing Penicillin Binding Proteins (PBPs) are not properly localized. This suggests that there may be a maximum cell diameter that allows formation of a Z-ring capable of recruiting the other components of the divisome and of driving cytokinesis. We propose that the major role of EzrA in S. aureus is in cell size homeostasis.
publishDate 2011
dc.date.none.fl_str_mv 2011
2011-01-01T00:00:00Z
2012-02-24T17:44:33Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.18/632
url http://hdl.handle.net/10400.18/632
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv PLoS One. 2011;6(11):e27542. Epub 2011 Nov 14
1932-6203
doi:10.1371/journal.pone.0027542
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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