Screening Mediterranean shrubs selected by browsing goats against gastrointestinal strongyles

Detalhes bibliográficos
Autor(a) principal: Padre, Ludovina
Data de Publicação: 2020
Outros Autores: Costa, Claudia, Belo, Ana Teresa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10174/34260
Resumo: Ethanolic extracts from the main species selected by browsing Charnequeira goats on Mediterranean vegetation were assayed for a rapid screening of their anthelmintic potential against gastrointestinal (GI) strongyles. Shrubs selected included Olea europeae var. sylvestris (OS), Quercus coccifera (QC), Pistacia lentiscus, Rhamnus alaternus and Rhamnus lycioides (RL). Forage species included Cichorium intybus (CI) and Hedysarum coronarium (HC). Extract concentrations were chosen taking as reference the fecal total phenolics content (TP) from those goats after a five-month browsing period (8.4 mg GAE/g DM). A quantitative coproculture method was adapted using faeces obtained from naturally infected goats, not dewormed or having access to browse (reference group); larvae culture was performed in triplicate for three different TP concentrations (25, 10 and 5 mg GAE/g DM) against a distilled water control, for a 7-day incubation period at 27 °C. The number of larvae developed was counted differentiating total and live larvae per gram of faeces (LDPG). The efficacy of each extract and concentration, as percentage of reduction in LDPG against control, was determined. Data was log transformed and submitted to analysis of variance. Means were compared by Tukey test at 5% significance level.A reduction in LDPG was observed in all extracts being significant (P<0.05) for the highest concentration. The extracts’ efficacy over exogenous forms showed not only a reduction of total LDPG but, for some, also a high level of larvae mortality, resulting in reduction of live infective larvae (L3). These results indicate that some extracts, besides their ovicidal activity, have also larvicidal potential. Extracts efficacy for total LDPG was higher for QC (82%), OS (80%), HC and RL (76%) and CI (69%). Reduction of live L3 was higher for OS (90%) and HC (80%). Results obtained on these extracts potential in reducing exogenous forms, namely L3 forms of GI strongyles, is an important aspect in the control of these parasitic populations, as it results in lower pasture contamination and consequently a lower rate of reinfection of the animals. Funding obtained from project VegMedCabras – ALT20-03-0145-FEDER-000009.
id RCAP_579925bfb5c07e145d1e546b854d1c7d
oai_identifier_str oai:dspace.uevora.pt:10174/34260
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Screening Mediterranean shrubs selected by browsing goats against gastrointestinal strongylesMediterranean shrubsgastrointestinal strongylesgoatsEthanolic extracts from the main species selected by browsing Charnequeira goats on Mediterranean vegetation were assayed for a rapid screening of their anthelmintic potential against gastrointestinal (GI) strongyles. Shrubs selected included Olea europeae var. sylvestris (OS), Quercus coccifera (QC), Pistacia lentiscus, Rhamnus alaternus and Rhamnus lycioides (RL). Forage species included Cichorium intybus (CI) and Hedysarum coronarium (HC). Extract concentrations were chosen taking as reference the fecal total phenolics content (TP) from those goats after a five-month browsing period (8.4 mg GAE/g DM). A quantitative coproculture method was adapted using faeces obtained from naturally infected goats, not dewormed or having access to browse (reference group); larvae culture was performed in triplicate for three different TP concentrations (25, 10 and 5 mg GAE/g DM) against a distilled water control, for a 7-day incubation period at 27 °C. The number of larvae developed was counted differentiating total and live larvae per gram of faeces (LDPG). The efficacy of each extract and concentration, as percentage of reduction in LDPG against control, was determined. Data was log transformed and submitted to analysis of variance. Means were compared by Tukey test at 5% significance level.A reduction in LDPG was observed in all extracts being significant (P<0.05) for the highest concentration. The extracts’ efficacy over exogenous forms showed not only a reduction of total LDPG but, for some, also a high level of larvae mortality, resulting in reduction of live infective larvae (L3). These results indicate that some extracts, besides their ovicidal activity, have also larvicidal potential. Extracts efficacy for total LDPG was higher for QC (82%), OS (80%), HC and RL (76%) and CI (69%). Reduction of live L3 was higher for OS (90%) and HC (80%). Results obtained on these extracts potential in reducing exogenous forms, namely L3 forms of GI strongyles, is an important aspect in the control of these parasitic populations, as it results in lower pasture contamination and consequently a lower rate of reinfection of the animals. Funding obtained from project VegMedCabras – ALT20-03-0145-FEDER-000009.EAAP - European Federation of Animal Science2023-02-13T17:29:19Z2023-02-132020-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10174/34260http://hdl.handle.net/10174/34260engPadre L., Costa C., Belo A.T., 2020.Screening Mediterranean shrubs selected by browsing goats against gastrointestinal strongyles. 71st Anual Meeting, Virtual Meeting, 1-4 December, EAAP - European Federation of Animal Sciencehttps://meetings.eaap.org/wp-content/uploads/2021/09/2020-book-of-abstracts.pdflpadre@uevora.ptclaudia.dc.costa@gmail.comanateresa.belo@iniav.pt384Padre, LudovinaCosta, ClaudiaBelo, Ana Teresainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T19:36:19Zoai:dspace.uevora.pt:10174/34260Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:22:44.694646Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Screening Mediterranean shrubs selected by browsing goats against gastrointestinal strongyles
title Screening Mediterranean shrubs selected by browsing goats against gastrointestinal strongyles
spellingShingle Screening Mediterranean shrubs selected by browsing goats against gastrointestinal strongyles
Padre, Ludovina
Mediterranean shrubs
gastrointestinal strongyles
goats
title_short Screening Mediterranean shrubs selected by browsing goats against gastrointestinal strongyles
title_full Screening Mediterranean shrubs selected by browsing goats against gastrointestinal strongyles
title_fullStr Screening Mediterranean shrubs selected by browsing goats against gastrointestinal strongyles
title_full_unstemmed Screening Mediterranean shrubs selected by browsing goats against gastrointestinal strongyles
title_sort Screening Mediterranean shrubs selected by browsing goats against gastrointestinal strongyles
author Padre, Ludovina
author_facet Padre, Ludovina
Costa, Claudia
Belo, Ana Teresa
author_role author
author2 Costa, Claudia
Belo, Ana Teresa
author2_role author
author
dc.contributor.author.fl_str_mv Padre, Ludovina
Costa, Claudia
Belo, Ana Teresa
dc.subject.por.fl_str_mv Mediterranean shrubs
gastrointestinal strongyles
goats
topic Mediterranean shrubs
gastrointestinal strongyles
goats
description Ethanolic extracts from the main species selected by browsing Charnequeira goats on Mediterranean vegetation were assayed for a rapid screening of their anthelmintic potential against gastrointestinal (GI) strongyles. Shrubs selected included Olea europeae var. sylvestris (OS), Quercus coccifera (QC), Pistacia lentiscus, Rhamnus alaternus and Rhamnus lycioides (RL). Forage species included Cichorium intybus (CI) and Hedysarum coronarium (HC). Extract concentrations were chosen taking as reference the fecal total phenolics content (TP) from those goats after a five-month browsing period (8.4 mg GAE/g DM). A quantitative coproculture method was adapted using faeces obtained from naturally infected goats, not dewormed or having access to browse (reference group); larvae culture was performed in triplicate for three different TP concentrations (25, 10 and 5 mg GAE/g DM) against a distilled water control, for a 7-day incubation period at 27 °C. The number of larvae developed was counted differentiating total and live larvae per gram of faeces (LDPG). The efficacy of each extract and concentration, as percentage of reduction in LDPG against control, was determined. Data was log transformed and submitted to analysis of variance. Means were compared by Tukey test at 5% significance level.A reduction in LDPG was observed in all extracts being significant (P<0.05) for the highest concentration. The extracts’ efficacy over exogenous forms showed not only a reduction of total LDPG but, for some, also a high level of larvae mortality, resulting in reduction of live infective larvae (L3). These results indicate that some extracts, besides their ovicidal activity, have also larvicidal potential. Extracts efficacy for total LDPG was higher for QC (82%), OS (80%), HC and RL (76%) and CI (69%). Reduction of live L3 was higher for OS (90%) and HC (80%). Results obtained on these extracts potential in reducing exogenous forms, namely L3 forms of GI strongyles, is an important aspect in the control of these parasitic populations, as it results in lower pasture contamination and consequently a lower rate of reinfection of the animals. Funding obtained from project VegMedCabras – ALT20-03-0145-FEDER-000009.
publishDate 2020
dc.date.none.fl_str_mv 2020-12-01T00:00:00Z
2023-02-13T17:29:19Z
2023-02-13
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10174/34260
http://hdl.handle.net/10174/34260
url http://hdl.handle.net/10174/34260
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Padre L., Costa C., Belo A.T., 2020.Screening Mediterranean shrubs selected by browsing goats against gastrointestinal strongyles. 71st Anual Meeting, Virtual Meeting, 1-4 December, EAAP - European Federation of Animal Science
https://meetings.eaap.org/wp-content/uploads/2021/09/2020-book-of-abstracts.pdf
lpadre@uevora.pt
claudia.dc.costa@gmail.com
anateresa.belo@iniav.pt
384
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv EAAP - European Federation of Animal Science
publisher.none.fl_str_mv EAAP - European Federation of Animal Science
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799136709112233984