Convection patterns gradients of non-living and living micro-entities in hydrogels
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.21/12598 |
Resumo: | Inducing thermal gradients in two injected fluid systems results in the temporal formation of mixing conductive streams. If preserved through sol-gel transition, this mechanism can be used to drive and pattern non-living and living entities in mixed hydrogels. Interfaces are vital in nature, where gradients of non-living and living entities build distinct yet continuous integrated living tissues. However, the common tissue fabrication methodologies often result in dissimilar interfaces, lacking continuity through the interfaced engineered tissues. Thus, there is an urgent need for the fabrication of heterotypic but continuous engineered tissues with spatial control over biomimetic features. Here, we demonstrate the influence of gel injection temperature on the patterning of gradients of non-living and living entities. The experimental part was confirmed by numerical modelling, showing the formation of convective lines which spatially drive microscale microparticle and cells when different temperatures are applied in the sequential injection of two gels. Based on this finding, pure gellan gum (GG) and blended GG with methacrylated gelatin (Ge1MA) systems were used to program the formation of gradient features in hydrogels, such as microparticle and cells distribution patterns, polymeric bioactivity, degradation, controlled release, and stiffness. The correlation between gel injection temperature and gradients formation can be applied to tissue interface modelling, regeneration, drug release systems, and broader materials engineering fields. |
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Convection patterns gradients of non-living and living micro-entities in hydrogelsGradient structureThermal convectionTemperature patternsSoft hydrogelsTissue engineeringInducing thermal gradients in two injected fluid systems results in the temporal formation of mixing conductive streams. If preserved through sol-gel transition, this mechanism can be used to drive and pattern non-living and living entities in mixed hydrogels. Interfaces are vital in nature, where gradients of non-living and living entities build distinct yet continuous integrated living tissues. However, the common tissue fabrication methodologies often result in dissimilar interfaces, lacking continuity through the interfaced engineered tissues. Thus, there is an urgent need for the fabrication of heterotypic but continuous engineered tissues with spatial control over biomimetic features. Here, we demonstrate the influence of gel injection temperature on the patterning of gradients of non-living and living entities. The experimental part was confirmed by numerical modelling, showing the formation of convective lines which spatially drive microscale microparticle and cells when different temperatures are applied in the sequential injection of two gels. Based on this finding, pure gellan gum (GG) and blended GG with methacrylated gelatin (Ge1MA) systems were used to program the formation of gradient features in hydrogels, such as microparticle and cells distribution patterns, polymeric bioactivity, degradation, controlled release, and stiffness. The correlation between gel injection temperature and gradients formation can be applied to tissue interface modelling, regeneration, drug release systems, and broader materials engineering fields.ElsevierRCIPLCanadas, Raphaël F.Patricio, PedroBrancato, VirginiaGasperini, LucaCaballero, DavidPires, Ricardo A.Costa, JoãoPereira, HélderYong, Pingda Silva, Lucília P.Chen, JieKundu, Subhas C.Araujo, NunoReis, Rui L.Marques, APOliveira, Joaquim M.2021-01-11T12:04:50Z2020-122020-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.21/12598engCANADAS, Rhafael F.; [et al] – Convection patterns gradients of non-living and living micro-entities in hydrogels. Applied Materials Today. ISSN 2352-9407. Vol. 21 (2020), pp. 1-182352-940710.1016/j.apmt.2020.100859metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-08-03T10:05:49Zoai:repositorio.ipl.pt:10400.21/12598Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:20:39.352409Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Convection patterns gradients of non-living and living micro-entities in hydrogels |
title |
Convection patterns gradients of non-living and living micro-entities in hydrogels |
spellingShingle |
Convection patterns gradients of non-living and living micro-entities in hydrogels Canadas, Raphaël F. Gradient structure Thermal convection Temperature patterns Soft hydrogels Tissue engineering |
title_short |
Convection patterns gradients of non-living and living micro-entities in hydrogels |
title_full |
Convection patterns gradients of non-living and living micro-entities in hydrogels |
title_fullStr |
Convection patterns gradients of non-living and living micro-entities in hydrogels |
title_full_unstemmed |
Convection patterns gradients of non-living and living micro-entities in hydrogels |
title_sort |
Convection patterns gradients of non-living and living micro-entities in hydrogels |
author |
Canadas, Raphaël F. |
author_facet |
Canadas, Raphaël F. Patricio, Pedro Brancato, Virginia Gasperini, Luca Caballero, David Pires, Ricardo A. Costa, João Pereira, Hélder Yong, Ping da Silva, Lucília P. Chen, Jie Kundu, Subhas C. Araujo, Nuno Reis, Rui L. Marques, AP Oliveira, Joaquim M. |
author_role |
author |
author2 |
Patricio, Pedro Brancato, Virginia Gasperini, Luca Caballero, David Pires, Ricardo A. Costa, João Pereira, Hélder Yong, Ping da Silva, Lucília P. Chen, Jie Kundu, Subhas C. Araujo, Nuno Reis, Rui L. Marques, AP Oliveira, Joaquim M. |
author2_role |
author author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
RCIPL |
dc.contributor.author.fl_str_mv |
Canadas, Raphaël F. Patricio, Pedro Brancato, Virginia Gasperini, Luca Caballero, David Pires, Ricardo A. Costa, João Pereira, Hélder Yong, Ping da Silva, Lucília P. Chen, Jie Kundu, Subhas C. Araujo, Nuno Reis, Rui L. Marques, AP Oliveira, Joaquim M. |
dc.subject.por.fl_str_mv |
Gradient structure Thermal convection Temperature patterns Soft hydrogels Tissue engineering |
topic |
Gradient structure Thermal convection Temperature patterns Soft hydrogels Tissue engineering |
description |
Inducing thermal gradients in two injected fluid systems results in the temporal formation of mixing conductive streams. If preserved through sol-gel transition, this mechanism can be used to drive and pattern non-living and living entities in mixed hydrogels. Interfaces are vital in nature, where gradients of non-living and living entities build distinct yet continuous integrated living tissues. However, the common tissue fabrication methodologies often result in dissimilar interfaces, lacking continuity through the interfaced engineered tissues. Thus, there is an urgent need for the fabrication of heterotypic but continuous engineered tissues with spatial control over biomimetic features. Here, we demonstrate the influence of gel injection temperature on the patterning of gradients of non-living and living entities. The experimental part was confirmed by numerical modelling, showing the formation of convective lines which spatially drive microscale microparticle and cells when different temperatures are applied in the sequential injection of two gels. Based on this finding, pure gellan gum (GG) and blended GG with methacrylated gelatin (Ge1MA) systems were used to program the formation of gradient features in hydrogels, such as microparticle and cells distribution patterns, polymeric bioactivity, degradation, controlled release, and stiffness. The correlation between gel injection temperature and gradients formation can be applied to tissue interface modelling, regeneration, drug release systems, and broader materials engineering fields. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12 2020-12-01T00:00:00Z 2021-01-11T12:04:50Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.21/12598 |
url |
http://hdl.handle.net/10400.21/12598 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
CANADAS, Rhafael F.; [et al] – Convection patterns gradients of non-living and living micro-entities in hydrogels. Applied Materials Today. ISSN 2352-9407. Vol. 21 (2020), pp. 1-18 2352-9407 10.1016/j.apmt.2020.100859 |
dc.rights.driver.fl_str_mv |
metadata only access info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
metadata only access |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799133475653025792 |