Fatal cases of Theileria annulata infection in calves in Portugal associated with neoplastic-like lymphoid cell proliferation
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10174/4023 |
Resumo: | The authors describe 15 cases of acute lethal infection of calves (≤ 4 months of age) by the protozoan parasite Theileria annulata in the south of Portugal. Calves developed multifocal to coalescent nodular skin lesions, similar to multicentric malignant lymphoma. Infestation with ticks (genus Hyalomma) was intense. Theileria was seen in blood and lymph node smears, and Theileria annulata infection was confirmed by isolation of schizont-transformed cells and sequencing of hypervariable region 4 of the 18S rRNA gene. At necropsy, hemorrhagic nodules or nodules with a hemorrhagic halo were seen, particularly in the skin, subcutaneous tissue, skeletal and cardiac muscle, pharynx, trachea and intestinal serosa. Histologically nodules were formed by large, round, lymphoblastoid neoplastic-like cells. Immunohistochemistry (IHC) identified these cells as mostly CD3 positive T lymphocytes and MAC387 positive macrophages. A marker for B lymphocytes (CD79αcy) labelled very few cells. Theileria annulata infected cells in these nodules were also identified by IHC, through the use of two monoclonal antibodies (1C7 and 1C12) diagnostic for the parasite. It was concluded that the pathological changes observed in the different organs and tissues were caused by proliferation of schizont-infected macrophages, which subsequently stimulate a severe uncontrolled proliferation of uninfected T lymphocytes. |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Fatal cases of Theileria annulata infection in calves in Portugal associated with neoplastic-like lymphoid cell proliferationlymphoid cell proliferationtropical theileriosisThe authors describe 15 cases of acute lethal infection of calves (≤ 4 months of age) by the protozoan parasite Theileria annulata in the south of Portugal. Calves developed multifocal to coalescent nodular skin lesions, similar to multicentric malignant lymphoma. Infestation with ticks (genus Hyalomma) was intense. Theileria was seen in blood and lymph node smears, and Theileria annulata infection was confirmed by isolation of schizont-transformed cells and sequencing of hypervariable region 4 of the 18S rRNA gene. At necropsy, hemorrhagic nodules or nodules with a hemorrhagic halo were seen, particularly in the skin, subcutaneous tissue, skeletal and cardiac muscle, pharynx, trachea and intestinal serosa. Histologically nodules were formed by large, round, lymphoblastoid neoplastic-like cells. Immunohistochemistry (IHC) identified these cells as mostly CD3 positive T lymphocytes and MAC387 positive macrophages. A marker for B lymphocytes (CD79αcy) labelled very few cells. Theileria annulata infected cells in these nodules were also identified by IHC, through the use of two monoclonal antibodies (1C7 and 1C12) diagnostic for the parasite. It was concluded that the pathological changes observed in the different organs and tissues were caused by proliferation of schizont-infected macrophages, which subsequently stimulate a severe uncontrolled proliferation of uninfected T lymphocytes.Journal of Veterinary Science 11 (1), 27-342012-01-23T14:44:29Z2012-01-232010-03-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10174/4023http://hdl.handle.net/10174/4023engFatal cases of Theileria annulata infection in calves in Portugal associated with neoplastic-like lymphoid cell proliferation. Sandra Branco, João Orvalho, Alexandre Leitão, Isadora Pereira, Manuel Malta, Isabel Mariano, Tânia Carvalho, Rui Baptista, Brian R. Shiels, Maria C. Peleteiro (2010). Journal of Veterinary Science 11 (1), 27-34http://www.vetsci.org.smbb@uevora.pt386Branco, SandraOrvalho, JoãoLeitão, AlexandrePereira, IsadoraMalta, ManuelMariano, IsabelCarvalho, TâniaBaptista, RuiShiels, Brian R.Peleteiro, Maria C.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T18:41:37Zoai:dspace.uevora.pt:10174/4023Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T00:59:21.724500Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Fatal cases of Theileria annulata infection in calves in Portugal associated with neoplastic-like lymphoid cell proliferation |
title |
Fatal cases of Theileria annulata infection in calves in Portugal associated with neoplastic-like lymphoid cell proliferation |
spellingShingle |
Fatal cases of Theileria annulata infection in calves in Portugal associated with neoplastic-like lymphoid cell proliferation Branco, Sandra lymphoid cell proliferation tropical theileriosis |
title_short |
Fatal cases of Theileria annulata infection in calves in Portugal associated with neoplastic-like lymphoid cell proliferation |
title_full |
Fatal cases of Theileria annulata infection in calves in Portugal associated with neoplastic-like lymphoid cell proliferation |
title_fullStr |
Fatal cases of Theileria annulata infection in calves in Portugal associated with neoplastic-like lymphoid cell proliferation |
title_full_unstemmed |
Fatal cases of Theileria annulata infection in calves in Portugal associated with neoplastic-like lymphoid cell proliferation |
title_sort |
Fatal cases of Theileria annulata infection in calves in Portugal associated with neoplastic-like lymphoid cell proliferation |
author |
Branco, Sandra |
author_facet |
Branco, Sandra Orvalho, João Leitão, Alexandre Pereira, Isadora Malta, Manuel Mariano, Isabel Carvalho, Tânia Baptista, Rui Shiels, Brian R. Peleteiro, Maria C. |
author_role |
author |
author2 |
Orvalho, João Leitão, Alexandre Pereira, Isadora Malta, Manuel Mariano, Isabel Carvalho, Tânia Baptista, Rui Shiels, Brian R. Peleteiro, Maria C. |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Branco, Sandra Orvalho, João Leitão, Alexandre Pereira, Isadora Malta, Manuel Mariano, Isabel Carvalho, Tânia Baptista, Rui Shiels, Brian R. Peleteiro, Maria C. |
dc.subject.por.fl_str_mv |
lymphoid cell proliferation tropical theileriosis |
topic |
lymphoid cell proliferation tropical theileriosis |
description |
The authors describe 15 cases of acute lethal infection of calves (≤ 4 months of age) by the protozoan parasite Theileria annulata in the south of Portugal. Calves developed multifocal to coalescent nodular skin lesions, similar to multicentric malignant lymphoma. Infestation with ticks (genus Hyalomma) was intense. Theileria was seen in blood and lymph node smears, and Theileria annulata infection was confirmed by isolation of schizont-transformed cells and sequencing of hypervariable region 4 of the 18S rRNA gene. At necropsy, hemorrhagic nodules or nodules with a hemorrhagic halo were seen, particularly in the skin, subcutaneous tissue, skeletal and cardiac muscle, pharynx, trachea and intestinal serosa. Histologically nodules were formed by large, round, lymphoblastoid neoplastic-like cells. Immunohistochemistry (IHC) identified these cells as mostly CD3 positive T lymphocytes and MAC387 positive macrophages. A marker for B lymphocytes (CD79αcy) labelled very few cells. Theileria annulata infected cells in these nodules were also identified by IHC, through the use of two monoclonal antibodies (1C7 and 1C12) diagnostic for the parasite. It was concluded that the pathological changes observed in the different organs and tissues were caused by proliferation of schizont-infected macrophages, which subsequently stimulate a severe uncontrolled proliferation of uninfected T lymphocytes. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-03-01T00:00:00Z 2012-01-23T14:44:29Z 2012-01-23 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10174/4023 http://hdl.handle.net/10174/4023 |
url |
http://hdl.handle.net/10174/4023 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Fatal cases of Theileria annulata infection in calves in Portugal associated with neoplastic-like lymphoid cell proliferation. Sandra Branco, João Orvalho, Alexandre Leitão, Isadora Pereira, Manuel Malta, Isabel Mariano, Tânia Carvalho, Rui Baptista, Brian R. Shiels, Maria C. Peleteiro (2010). Journal of Veterinary Science 11 (1), 27-34 http://www.vetsci.org. smbb@uevora.pt 386 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Journal of Veterinary Science 11 (1), 27-34 |
publisher.none.fl_str_mv |
Journal of Veterinary Science 11 (1), 27-34 |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799136476117598208 |