Fatal cases of Theileria annulata infection in calves in Portugal associated with neoplastic-like lymphoid cell proliferation

Detalhes bibliográficos
Autor(a) principal: Branco, Sandra
Data de Publicação: 2010
Outros Autores: Orvalho, João, Leitão, Alexandre, Pereira, Isadora, Malta, Manuel, Mariano, Isabel, Carvalho, Tânia, Baptista, Rui, Shiels, Brian R., Peleteiro, Maria C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10174/4023
Resumo: The authors describe 15 cases of acute lethal infection of calves (≤ 4 months of age) by the protozoan parasite Theileria annulata in the south of Portugal. Calves developed multifocal to coalescent nodular skin lesions, similar to multicentric malignant lymphoma. Infestation with ticks (genus Hyalomma) was intense. Theileria was seen in blood and lymph node smears, and Theileria annulata infection was confirmed by isolation of schizont-transformed cells and sequencing of hypervariable region 4 of the 18S rRNA gene. At necropsy, hemorrhagic nodules or nodules with a hemorrhagic halo were seen, particularly in the skin, subcutaneous tissue, skeletal and cardiac muscle, pharynx, trachea and intestinal serosa. Histologically nodules were formed by large, round, lymphoblastoid neoplastic-like cells. Immunohistochemistry (IHC) identified these cells as mostly CD3 positive T lymphocytes and MAC387 positive macrophages. A marker for B lymphocytes (CD79αcy) labelled very few cells. Theileria annulata infected cells in these nodules were also identified by IHC, through the use of two monoclonal antibodies (1C7 and 1C12) diagnostic for the parasite. It was concluded that the pathological changes observed in the different organs and tissues were caused by proliferation of schizont-infected macrophages, which subsequently stimulate a severe uncontrolled proliferation of uninfected T lymphocytes.
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spelling Fatal cases of Theileria annulata infection in calves in Portugal associated with neoplastic-like lymphoid cell proliferationlymphoid cell proliferationtropical theileriosisThe authors describe 15 cases of acute lethal infection of calves (≤ 4 months of age) by the protozoan parasite Theileria annulata in the south of Portugal. Calves developed multifocal to coalescent nodular skin lesions, similar to multicentric malignant lymphoma. Infestation with ticks (genus Hyalomma) was intense. Theileria was seen in blood and lymph node smears, and Theileria annulata infection was confirmed by isolation of schizont-transformed cells and sequencing of hypervariable region 4 of the 18S rRNA gene. At necropsy, hemorrhagic nodules or nodules with a hemorrhagic halo were seen, particularly in the skin, subcutaneous tissue, skeletal and cardiac muscle, pharynx, trachea and intestinal serosa. Histologically nodules were formed by large, round, lymphoblastoid neoplastic-like cells. Immunohistochemistry (IHC) identified these cells as mostly CD3 positive T lymphocytes and MAC387 positive macrophages. A marker for B lymphocytes (CD79αcy) labelled very few cells. Theileria annulata infected cells in these nodules were also identified by IHC, through the use of two monoclonal antibodies (1C7 and 1C12) diagnostic for the parasite. It was concluded that the pathological changes observed in the different organs and tissues were caused by proliferation of schizont-infected macrophages, which subsequently stimulate a severe uncontrolled proliferation of uninfected T lymphocytes.Journal of Veterinary Science 11 (1), 27-342012-01-23T14:44:29Z2012-01-232010-03-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10174/4023http://hdl.handle.net/10174/4023engFatal cases of Theileria annulata infection in calves in Portugal associated with neoplastic-like lymphoid cell proliferation. Sandra Branco, João Orvalho, Alexandre Leitão, Isadora Pereira, Manuel Malta, Isabel Mariano, Tânia Carvalho, Rui Baptista, Brian R. Shiels, Maria C. Peleteiro (2010). Journal of Veterinary Science 11 (1), 27-34http://www.vetsci.org.smbb@uevora.pt386Branco, SandraOrvalho, JoãoLeitão, AlexandrePereira, IsadoraMalta, ManuelMariano, IsabelCarvalho, TâniaBaptista, RuiShiels, Brian R.Peleteiro, Maria C.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T18:41:37Zoai:dspace.uevora.pt:10174/4023Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T00:59:21.724500Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Fatal cases of Theileria annulata infection in calves in Portugal associated with neoplastic-like lymphoid cell proliferation
title Fatal cases of Theileria annulata infection in calves in Portugal associated with neoplastic-like lymphoid cell proliferation
spellingShingle Fatal cases of Theileria annulata infection in calves in Portugal associated with neoplastic-like lymphoid cell proliferation
Branco, Sandra
lymphoid cell proliferation
tropical theileriosis
title_short Fatal cases of Theileria annulata infection in calves in Portugal associated with neoplastic-like lymphoid cell proliferation
title_full Fatal cases of Theileria annulata infection in calves in Portugal associated with neoplastic-like lymphoid cell proliferation
title_fullStr Fatal cases of Theileria annulata infection in calves in Portugal associated with neoplastic-like lymphoid cell proliferation
title_full_unstemmed Fatal cases of Theileria annulata infection in calves in Portugal associated with neoplastic-like lymphoid cell proliferation
title_sort Fatal cases of Theileria annulata infection in calves in Portugal associated with neoplastic-like lymphoid cell proliferation
author Branco, Sandra
author_facet Branco, Sandra
Orvalho, João
Leitão, Alexandre
Pereira, Isadora
Malta, Manuel
Mariano, Isabel
Carvalho, Tânia
Baptista, Rui
Shiels, Brian R.
Peleteiro, Maria C.
author_role author
author2 Orvalho, João
Leitão, Alexandre
Pereira, Isadora
Malta, Manuel
Mariano, Isabel
Carvalho, Tânia
Baptista, Rui
Shiels, Brian R.
Peleteiro, Maria C.
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Branco, Sandra
Orvalho, João
Leitão, Alexandre
Pereira, Isadora
Malta, Manuel
Mariano, Isabel
Carvalho, Tânia
Baptista, Rui
Shiels, Brian R.
Peleteiro, Maria C.
dc.subject.por.fl_str_mv lymphoid cell proliferation
tropical theileriosis
topic lymphoid cell proliferation
tropical theileriosis
description The authors describe 15 cases of acute lethal infection of calves (≤ 4 months of age) by the protozoan parasite Theileria annulata in the south of Portugal. Calves developed multifocal to coalescent nodular skin lesions, similar to multicentric malignant lymphoma. Infestation with ticks (genus Hyalomma) was intense. Theileria was seen in blood and lymph node smears, and Theileria annulata infection was confirmed by isolation of schizont-transformed cells and sequencing of hypervariable region 4 of the 18S rRNA gene. At necropsy, hemorrhagic nodules or nodules with a hemorrhagic halo were seen, particularly in the skin, subcutaneous tissue, skeletal and cardiac muscle, pharynx, trachea and intestinal serosa. Histologically nodules were formed by large, round, lymphoblastoid neoplastic-like cells. Immunohistochemistry (IHC) identified these cells as mostly CD3 positive T lymphocytes and MAC387 positive macrophages. A marker for B lymphocytes (CD79αcy) labelled very few cells. Theileria annulata infected cells in these nodules were also identified by IHC, through the use of two monoclonal antibodies (1C7 and 1C12) diagnostic for the parasite. It was concluded that the pathological changes observed in the different organs and tissues were caused by proliferation of schizont-infected macrophages, which subsequently stimulate a severe uncontrolled proliferation of uninfected T lymphocytes.
publishDate 2010
dc.date.none.fl_str_mv 2010-03-01T00:00:00Z
2012-01-23T14:44:29Z
2012-01-23
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10174/4023
http://hdl.handle.net/10174/4023
url http://hdl.handle.net/10174/4023
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Fatal cases of Theileria annulata infection in calves in Portugal associated with neoplastic-like lymphoid cell proliferation. Sandra Branco, João Orvalho, Alexandre Leitão, Isadora Pereira, Manuel Malta, Isabel Mariano, Tânia Carvalho, Rui Baptista, Brian R. Shiels, Maria C. Peleteiro (2010). Journal of Veterinary Science 11 (1), 27-34
http://www.vetsci.org.
smbb@uevora.pt
386
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Journal of Veterinary Science 11 (1), 27-34
publisher.none.fl_str_mv Journal of Veterinary Science 11 (1), 27-34
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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