Altered bone microarchitecture in a type 1 diabetes mouse model Ins2 (Akita)

Detalhes bibliográficos
Autor(a) principal: Pires De Carvalho, Filipe Ricardo
Data de Publicação: 2018
Outros Autores: Calado, Sofia, Silva, Gabriela A., Diogo, Gabriela S., Moreira da Silva, Joana, Reis, Rui L., Cancela, M. Leonor, Gavaia, Paulo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.1/14540
Resumo: Type 1 diabetes mellitus (T1DM) has been associated to several cartilage and bone alterations including growth retardation, increased fracture risk, and bone loss. To determine the effect of long term diabetes on bone we used adult and aging Ins2 Akita mice that developed T1DM around 3-4 weeks after birth. Both Ins2 Akita and wild-type (WT) mice were analyzed at 4, 6, and 12 months to assess bone parameters such as femur length, growth plate thickness and number of mature and preapoptotic chondrocytes. In addition, bone microarchitecture of the cortical and trabecular regions was measured by microcomputed tomography and gene expression of Adamst-5, Col2, Igf1, Runx2, Acp5, and Oc was quantified by quantitative real-time polymerase chain reaction. Ins2 Akita mice showed a decreased longitudinal growth of the femur that was related to decreased growth plate thickness, lower number of chondrocytes and to a higher number of preapoptotic cells. These changes were associated with higher expression of Adamst-5, suggesting higher cartilage degradation, and with low expression levels of Igf1 and Col2 that reflect the decreased growth ability of diabetic mice. Ins2 Akita bone morphology was characterized by low cortical bone area (Ct.Ar) but higher trabecular bone volume (BV/TV) and expression analysis showed a downregulation of bone markers Acp5, Oc, and Runx2. Serum levels of insulin and leptin were found to be reduced at all-time points Ins2 Akita . We suggest that Ins2 Akita mice bone phenotype is caused by lower bone formation and even lower bone resorption due to insulin deficiency and to a possible relation with low leptin signaling.
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spelling Altered bone microarchitecture in a type 1 diabetes mouse model Ins2 (Akita)AnimalsApoptosisBiomarkersBlood glucoseBody weightCancellous boneCartilageCortical boneDiabetes mellitusDisease modelsFemurGene expression regulationGrowth plateInsulinLeptinMaleMiceOrgan sizeTartrate-resistant acid phosphataseType 1Inbred C57BLAnimalType 1 diabetes mellitus (T1DM) has been associated to several cartilage and bone alterations including growth retardation, increased fracture risk, and bone loss. To determine the effect of long term diabetes on bone we used adult and aging Ins2 Akita mice that developed T1DM around 3-4 weeks after birth. Both Ins2 Akita and wild-type (WT) mice were analyzed at 4, 6, and 12 months to assess bone parameters such as femur length, growth plate thickness and number of mature and preapoptotic chondrocytes. In addition, bone microarchitecture of the cortical and trabecular regions was measured by microcomputed tomography and gene expression of Adamst-5, Col2, Igf1, Runx2, Acp5, and Oc was quantified by quantitative real-time polymerase chain reaction. Ins2 Akita mice showed a decreased longitudinal growth of the femur that was related to decreased growth plate thickness, lower number of chondrocytes and to a higher number of preapoptotic cells. These changes were associated with higher expression of Adamst-5, suggesting higher cartilage degradation, and with low expression levels of Igf1 and Col2 that reflect the decreased growth ability of diabetic mice. Ins2 Akita bone morphology was characterized by low cortical bone area (Ct.Ar) but higher trabecular bone volume (BV/TV) and expression analysis showed a downregulation of bone markers Acp5, Oc, and Runx2. Serum levels of insulin and leptin were found to be reduced at all-time points Ins2 Akita . We suggest that Ins2 Akita mice bone phenotype is caused by lower bone formation and even lower bone resorption due to insulin deficiency and to a possible relation with low leptin signaling.SFRH/BD/76873/2011/ SFRH/BD/76429/2011/ PEst-C/MAR/LA0015/2011WileySapientiaPires De Carvalho, Filipe RicardoCalado, SofiaSilva, Gabriela A.Diogo, Gabriela S.Moreira da Silva, JoanaReis, Rui L.Cancela, M. LeonorGavaia, Paulo2020-07-29T12:08:00Z20182018-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.1/14540eng0021-954110.1002/jcp.27617info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-24T10:26:51Zoai:sapientia.ualg.pt:10400.1/14540Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:05:33.959813Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Altered bone microarchitecture in a type 1 diabetes mouse model Ins2 (Akita)
title Altered bone microarchitecture in a type 1 diabetes mouse model Ins2 (Akita)
spellingShingle Altered bone microarchitecture in a type 1 diabetes mouse model Ins2 (Akita)
Pires De Carvalho, Filipe Ricardo
Animals
Apoptosis
Biomarkers
Blood glucose
Body weight
Cancellous bone
Cartilage
Cortical bone
Diabetes mellitus
Disease models
Femur
Gene expression regulation
Growth plate
Insulin
Leptin
Male
Mice
Organ size
Tartrate-resistant acid phosphatase
Type 1
Inbred C57BL
Animal
title_short Altered bone microarchitecture in a type 1 diabetes mouse model Ins2 (Akita)
title_full Altered bone microarchitecture in a type 1 diabetes mouse model Ins2 (Akita)
title_fullStr Altered bone microarchitecture in a type 1 diabetes mouse model Ins2 (Akita)
title_full_unstemmed Altered bone microarchitecture in a type 1 diabetes mouse model Ins2 (Akita)
title_sort Altered bone microarchitecture in a type 1 diabetes mouse model Ins2 (Akita)
author Pires De Carvalho, Filipe Ricardo
author_facet Pires De Carvalho, Filipe Ricardo
Calado, Sofia
Silva, Gabriela A.
Diogo, Gabriela S.
Moreira da Silva, Joana
Reis, Rui L.
Cancela, M. Leonor
Gavaia, Paulo
author_role author
author2 Calado, Sofia
Silva, Gabriela A.
Diogo, Gabriela S.
Moreira da Silva, Joana
Reis, Rui L.
Cancela, M. Leonor
Gavaia, Paulo
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Sapientia
dc.contributor.author.fl_str_mv Pires De Carvalho, Filipe Ricardo
Calado, Sofia
Silva, Gabriela A.
Diogo, Gabriela S.
Moreira da Silva, Joana
Reis, Rui L.
Cancela, M. Leonor
Gavaia, Paulo
dc.subject.por.fl_str_mv Animals
Apoptosis
Biomarkers
Blood glucose
Body weight
Cancellous bone
Cartilage
Cortical bone
Diabetes mellitus
Disease models
Femur
Gene expression regulation
Growth plate
Insulin
Leptin
Male
Mice
Organ size
Tartrate-resistant acid phosphatase
Type 1
Inbred C57BL
Animal
topic Animals
Apoptosis
Biomarkers
Blood glucose
Body weight
Cancellous bone
Cartilage
Cortical bone
Diabetes mellitus
Disease models
Femur
Gene expression regulation
Growth plate
Insulin
Leptin
Male
Mice
Organ size
Tartrate-resistant acid phosphatase
Type 1
Inbred C57BL
Animal
description Type 1 diabetes mellitus (T1DM) has been associated to several cartilage and bone alterations including growth retardation, increased fracture risk, and bone loss. To determine the effect of long term diabetes on bone we used adult and aging Ins2 Akita mice that developed T1DM around 3-4 weeks after birth. Both Ins2 Akita and wild-type (WT) mice were analyzed at 4, 6, and 12 months to assess bone parameters such as femur length, growth plate thickness and number of mature and preapoptotic chondrocytes. In addition, bone microarchitecture of the cortical and trabecular regions was measured by microcomputed tomography and gene expression of Adamst-5, Col2, Igf1, Runx2, Acp5, and Oc was quantified by quantitative real-time polymerase chain reaction. Ins2 Akita mice showed a decreased longitudinal growth of the femur that was related to decreased growth plate thickness, lower number of chondrocytes and to a higher number of preapoptotic cells. These changes were associated with higher expression of Adamst-5, suggesting higher cartilage degradation, and with low expression levels of Igf1 and Col2 that reflect the decreased growth ability of diabetic mice. Ins2 Akita bone morphology was characterized by low cortical bone area (Ct.Ar) but higher trabecular bone volume (BV/TV) and expression analysis showed a downregulation of bone markers Acp5, Oc, and Runx2. Serum levels of insulin and leptin were found to be reduced at all-time points Ins2 Akita . We suggest that Ins2 Akita mice bone phenotype is caused by lower bone formation and even lower bone resorption due to insulin deficiency and to a possible relation with low leptin signaling.
publishDate 2018
dc.date.none.fl_str_mv 2018
2018-01-01T00:00:00Z
2020-07-29T12:08:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.1/14540
url http://hdl.handle.net/10400.1/14540
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0021-9541
10.1002/jcp.27617
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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