From inception to implementation: strategies for setting up pulmonary telerehabilitation

Detalhes bibliográficos
Autor(a) principal: Santos, Catarina
Data de Publicação: 2022
Outros Autores: Rodrigues, Fatima, Caneiras, Catia, Bárbara, Cristina
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/53174
Resumo: Copyright © 2022 Santos, Rodrigues, Caneiras and Bárbara. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
id RCAP_5866240528a97a6d7a042155c0431632
oai_identifier_str oai:repositorio.ul.pt:10451/53174
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling From inception to implementation: strategies for setting up pulmonary telerehabilitationAMPKMembrane-less organellesMetforminMitochondriaProcessing bodiesSmaugUQCRC1Copyright © 2022 Santos, Rodrigues, Caneiras and Bárbara. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.Smaug is a conserved translational regulator that binds numerous mRNAs, including nuclear transcripts that encode mitochondrial enzymes. Smaug orthologs form cytosolic membrane-less organelles (MLOs) in several organisms and cell types. We have performed single-molecule fluorescence in situ hybridization (FISH) assays that revealed that SDHB and UQCRC1 mRNAs associate with Smaug1 bodies in U2OS cells. Loss of function of Smaug1 and Smaug2 (also known as SAMD4A and SAMD4B, respectively) affected both mitochondrial respiration and morphology of the mitochondrial network. Phenotype rescue by Smaug1 transfection depends on the presence of its RNA-binding domain. Moreover, we identified specific Smaug1 domains involved in MLO formation, and found that impaired Smaug1 MLO condensation correlates with mitochondrial defects. Mitochondrial complex I inhibition upon exposure to rotenone, but not strong mitochondrial uncoupling upon exposure to CCCP, rapidly induced the dissolution of Smaug1 MLOs. Metformin and rapamycin elicited similar effects, which were blocked by pharmacological inhibition of AMP-activated protein kinase (AMPK). Finally, we found that Smaug1 MLO dissolution weakens the interaction with target mRNAs, thus enabling their release. We propose that mitochondrial respiration and the AMPK-mTOR balance controls the condensation and dissolution of Smaug1 MLOs, thus regulating nuclear mRNAs that encode key mitochondrial proteins. This article has an associated First Person interview with the first authors of the paper.This work was supported by Fundação para a Ciência e Tecnologia (FCT) and Nippon Gases Portugal under the Ph.D. studentship in Industry grant PDE/BDE/127785/2016 awarded to CS.FrontiersRepositório da Universidade de LisboaSantos, CatarinaRodrigues, FatimaCaneiras, CatiaBárbara, Cristina2022-05-25T15:15:06Z20222022-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/53174engJ Cell Sci. 2022 Dec 1;135(1):jcs25359110.3389/fresc.2022.8301152673-6861info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-11-20T18:14:38Zoai:repositorio.ul.pt:10451/53174Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-11-20T18:14:38Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv From inception to implementation: strategies for setting up pulmonary telerehabilitation
title From inception to implementation: strategies for setting up pulmonary telerehabilitation
spellingShingle From inception to implementation: strategies for setting up pulmonary telerehabilitation
Santos, Catarina
AMPK
Membrane-less organelles
Metformin
Mitochondria
Processing bodies
Smaug
UQCRC1
title_short From inception to implementation: strategies for setting up pulmonary telerehabilitation
title_full From inception to implementation: strategies for setting up pulmonary telerehabilitation
title_fullStr From inception to implementation: strategies for setting up pulmonary telerehabilitation
title_full_unstemmed From inception to implementation: strategies for setting up pulmonary telerehabilitation
title_sort From inception to implementation: strategies for setting up pulmonary telerehabilitation
author Santos, Catarina
author_facet Santos, Catarina
Rodrigues, Fatima
Caneiras, Catia
Bárbara, Cristina
author_role author
author2 Rodrigues, Fatima
Caneiras, Catia
Bárbara, Cristina
author2_role author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Santos, Catarina
Rodrigues, Fatima
Caneiras, Catia
Bárbara, Cristina
dc.subject.por.fl_str_mv AMPK
Membrane-less organelles
Metformin
Mitochondria
Processing bodies
Smaug
UQCRC1
topic AMPK
Membrane-less organelles
Metformin
Mitochondria
Processing bodies
Smaug
UQCRC1
description Copyright © 2022 Santos, Rodrigues, Caneiras and Bárbara. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
publishDate 2022
dc.date.none.fl_str_mv 2022-05-25T15:15:06Z
2022
2022-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/53174
url http://hdl.handle.net/10451/53174
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv J Cell Sci. 2022 Dec 1;135(1):jcs253591
10.3389/fresc.2022.830115
2673-6861
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers
publisher.none.fl_str_mv Frontiers
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
_version_ 1817549189862653952

Registros relacionados