An EIS study of DNA-modified electrodes
Autor(a) principal: | |
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Data de Publicação: | 1999 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/5258 |
Resumo: | The preparation and conditioning of DNA-modified electrodes, in which glassy carbon electrodes are covered by a thick film of double-stranded DNA and subsequently conditioned in acetate buffer, has been followed with Electrochemical Impedance Spectroscopy. Spectra were recorded at different values of applied potential in order to probe the alterations occurring in the modifier layer. The alterations in the EIS response parallel the changes in voltammetric response and show that the modifier layer is a good conductor and that the behaviour as a DNA-biosensor is essentially due to surface phenomena and interaction with single-stranded DNA in solution during conditioning. |
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An EIS study of DNA-modified electrodesDNA-modified electrodesElectrochemical impedanceGuanineAdenineDouble-stranded DNASingle-stranded DNAThe preparation and conditioning of DNA-modified electrodes, in which glassy carbon electrodes are covered by a thick film of double-stranded DNA and subsequently conditioned in acetate buffer, has been followed with Electrochemical Impedance Spectroscopy. Spectra were recorded at different values of applied potential in order to probe the alterations occurring in the modifier layer. The alterations in the EIS response parallel the changes in voltammetric response and show that the modifier layer is a good conductor and that the behaviour as a DNA-biosensor is essentially due to surface phenomena and interaction with single-stranded DNA in solution during conditioning.http://www.sciencedirect.com/science/article/B6TG0-3X1WCNS-G/1/01970b94c35d7f299bd478182a5590c71999info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleaplication/PDFhttp://hdl.handle.net/10316/5258http://hdl.handle.net/10316/5258engElectrochimica Acta. 44:24 (1999) 4233-4239Brett, Christopher M. A.Brett, Ana Maria OliveiraSerrano, Sílvia H. P.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2021-10-26T13:12:16Zoai:estudogeral.uc.pt:10316/5258Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:01:37.568175Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
An EIS study of DNA-modified electrodes |
title |
An EIS study of DNA-modified electrodes |
spellingShingle |
An EIS study of DNA-modified electrodes Brett, Christopher M. A. DNA-modified electrodes Electrochemical impedance Guanine Adenine Double-stranded DNA Single-stranded DNA |
title_short |
An EIS study of DNA-modified electrodes |
title_full |
An EIS study of DNA-modified electrodes |
title_fullStr |
An EIS study of DNA-modified electrodes |
title_full_unstemmed |
An EIS study of DNA-modified electrodes |
title_sort |
An EIS study of DNA-modified electrodes |
author |
Brett, Christopher M. A. |
author_facet |
Brett, Christopher M. A. Brett, Ana Maria Oliveira Serrano, Sílvia H. P. |
author_role |
author |
author2 |
Brett, Ana Maria Oliveira Serrano, Sílvia H. P. |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Brett, Christopher M. A. Brett, Ana Maria Oliveira Serrano, Sílvia H. P. |
dc.subject.por.fl_str_mv |
DNA-modified electrodes Electrochemical impedance Guanine Adenine Double-stranded DNA Single-stranded DNA |
topic |
DNA-modified electrodes Electrochemical impedance Guanine Adenine Double-stranded DNA Single-stranded DNA |
description |
The preparation and conditioning of DNA-modified electrodes, in which glassy carbon electrodes are covered by a thick film of double-stranded DNA and subsequently conditioned in acetate buffer, has been followed with Electrochemical Impedance Spectroscopy. Spectra were recorded at different values of applied potential in order to probe the alterations occurring in the modifier layer. The alterations in the EIS response parallel the changes in voltammetric response and show that the modifier layer is a good conductor and that the behaviour as a DNA-biosensor is essentially due to surface phenomena and interaction with single-stranded DNA in solution during conditioning. |
publishDate |
1999 |
dc.date.none.fl_str_mv |
1999 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/5258 http://hdl.handle.net/10316/5258 |
url |
http://hdl.handle.net/10316/5258 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Electrochimica Acta. 44:24 (1999) 4233-4239 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
aplication/PDF |
dc.source.none.fl_str_mv |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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