Direct lipid and carotenoid extraction from Rhodosporidium toruloides broth culture after high pressure homogenization cell disruption: Strategies, methodologies, and yields
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.9/4014 |
Resumo: | ABSTRACT: Biodiesel produced from oleaginous microorganisms is considered an appealing option since oleaginous yeasts and microalgae can accumulate between 20 % and 80 % lipids of their dry cell weight. However, it is still not economically sustainable since production costs are still high. This work developed a new mild protocol using hexane or sunflower oil as extraction solvents to simultaneously extract lipids and carotenoids from Rhodosporidium toruloides biomass directly from the broth culture without using any harvesting technique after performing high pressure homogenization to disrupt the yeast cells. The highest results were obtained for the hexane extractions attaining 55.9 % of total lipids (w/w) and 111.5 mu g g-1 (eq. torulene) of carotenoids, which are higher values than the ones obtained using lipid extraction traditional methods. The process here presented is an easy, simple, inexpensive and environmentally friendly methodology which involves the simultaneous extraction of carotenoids and lipids directly from R. toruloides broth culture which has never been reported before and can greatly improve the economics of the biodiesel production process. |
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Direct lipid and carotenoid extraction from Rhodosporidium toruloides broth culture after high pressure homogenization cell disruption: Strategies, methodologies, and yieldsBiodiesel ProductionRhodosporidium toruloidesLipidsCarotenoidsLiquid-liquid extractionABSTRACT: Biodiesel produced from oleaginous microorganisms is considered an appealing option since oleaginous yeasts and microalgae can accumulate between 20 % and 80 % lipids of their dry cell weight. However, it is still not economically sustainable since production costs are still high. This work developed a new mild protocol using hexane or sunflower oil as extraction solvents to simultaneously extract lipids and carotenoids from Rhodosporidium toruloides biomass directly from the broth culture without using any harvesting technique after performing high pressure homogenization to disrupt the yeast cells. The highest results were obtained for the hexane extractions attaining 55.9 % of total lipids (w/w) and 111.5 mu g g-1 (eq. torulene) of carotenoids, which are higher values than the ones obtained using lipid extraction traditional methods. The process here presented is an easy, simple, inexpensive and environmentally friendly methodology which involves the simultaneous extraction of carotenoids and lipids directly from R. toruloides broth culture which has never been reported before and can greatly improve the economics of the biodiesel production process.ElsevierRepositório do LNEGDias, CarlaNobre, B. P.Santos, J. A. L.Silva, Teresa Lopes daReis, Alberto2023-02-10T16:22:06Z2022-122022-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.9/4014engDias, Carla... [et.al.] - Direct lipid and carotenoid extraction from Rhodosporidium toruloides broth culture after high pressure homogenization cell disruption: Strategies, methodologies, and yields. In: Biochemical Engineering Journal, 2022, vol. 189, article nº 1087121369-703X10.1016/j.bej.2022.1087121873-295Xinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-02-12T05:16:26Zoai:repositorio.lneg.pt:10400.9/4014Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:46:30.368881Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Direct lipid and carotenoid extraction from Rhodosporidium toruloides broth culture after high pressure homogenization cell disruption: Strategies, methodologies, and yields |
title |
Direct lipid and carotenoid extraction from Rhodosporidium toruloides broth culture after high pressure homogenization cell disruption: Strategies, methodologies, and yields |
spellingShingle |
Direct lipid and carotenoid extraction from Rhodosporidium toruloides broth culture after high pressure homogenization cell disruption: Strategies, methodologies, and yields Dias, Carla Biodiesel Production Rhodosporidium toruloides Lipids Carotenoids Liquid-liquid extraction |
title_short |
Direct lipid and carotenoid extraction from Rhodosporidium toruloides broth culture after high pressure homogenization cell disruption: Strategies, methodologies, and yields |
title_full |
Direct lipid and carotenoid extraction from Rhodosporidium toruloides broth culture after high pressure homogenization cell disruption: Strategies, methodologies, and yields |
title_fullStr |
Direct lipid and carotenoid extraction from Rhodosporidium toruloides broth culture after high pressure homogenization cell disruption: Strategies, methodologies, and yields |
title_full_unstemmed |
Direct lipid and carotenoid extraction from Rhodosporidium toruloides broth culture after high pressure homogenization cell disruption: Strategies, methodologies, and yields |
title_sort |
Direct lipid and carotenoid extraction from Rhodosporidium toruloides broth culture after high pressure homogenization cell disruption: Strategies, methodologies, and yields |
author |
Dias, Carla |
author_facet |
Dias, Carla Nobre, B. P. Santos, J. A. L. Silva, Teresa Lopes da Reis, Alberto |
author_role |
author |
author2 |
Nobre, B. P. Santos, J. A. L. Silva, Teresa Lopes da Reis, Alberto |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Repositório do LNEG |
dc.contributor.author.fl_str_mv |
Dias, Carla Nobre, B. P. Santos, J. A. L. Silva, Teresa Lopes da Reis, Alberto |
dc.subject.por.fl_str_mv |
Biodiesel Production Rhodosporidium toruloides Lipids Carotenoids Liquid-liquid extraction |
topic |
Biodiesel Production Rhodosporidium toruloides Lipids Carotenoids Liquid-liquid extraction |
description |
ABSTRACT: Biodiesel produced from oleaginous microorganisms is considered an appealing option since oleaginous yeasts and microalgae can accumulate between 20 % and 80 % lipids of their dry cell weight. However, it is still not economically sustainable since production costs are still high. This work developed a new mild protocol using hexane or sunflower oil as extraction solvents to simultaneously extract lipids and carotenoids from Rhodosporidium toruloides biomass directly from the broth culture without using any harvesting technique after performing high pressure homogenization to disrupt the yeast cells. The highest results were obtained for the hexane extractions attaining 55.9 % of total lipids (w/w) and 111.5 mu g g-1 (eq. torulene) of carotenoids, which are higher values than the ones obtained using lipid extraction traditional methods. The process here presented is an easy, simple, inexpensive and environmentally friendly methodology which involves the simultaneous extraction of carotenoids and lipids directly from R. toruloides broth culture which has never been reported before and can greatly improve the economics of the biodiesel production process. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-12 2022-12-01T00:00:00Z 2023-02-10T16:22:06Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.9/4014 |
url |
http://hdl.handle.net/10400.9/4014 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Dias, Carla... [et.al.] - Direct lipid and carotenoid extraction from Rhodosporidium toruloides broth culture after high pressure homogenization cell disruption: Strategies, methodologies, and yields. In: Biochemical Engineering Journal, 2022, vol. 189, article nº 108712 1369-703X 10.1016/j.bej.2022.108712 1873-295X |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799130936470667264 |