Changes in salivary protein composition of lambs supplemented with aerial parts and condensed tannins: extract from Cistus ladanifer L. - a preliminary study.
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10174/27366 https://doi.org/10.1007/s10457-019-00386-4 |
Resumo: | Cistus ladanifer L. is a shrub present in Mediterranean areas without major use, but which can be incorporated into ruminant diets, improving the digestive efficiency of dietary protein and product quality. The high levels of plant secondary metabolites, including condensed tannins, may be responsible for the beneficial properties of the plant, but can also reduce palatability. In this study, C. ladanifer was incorporated into lambs’ diets, either as aerial parts or as a condensed tannin extract, reaching 1.25% and 2.5% of condensed tannins of dry matter. Saliva was collected and electrophoretic profiles of both whole saliva and saliva after in vitro incubation with C. ladanifer tannins were compared. Animals receiving the aerial parts of C. ladanifer decreased feed ingestion. Differences in salivary protein profiles were observed for animals fed with the higher levels of aerial parts of the plant (CL2.5 group). Most of the lambs’ salivary proteins were present in the precipitate formed after tannin-saliva incubation, and one of the bands increased in CL2.5 group was present in high proportion in the precipitate. None of the protein bands stained pink with Coomassie Brilliant Blue, suggesting the absence, or low amounts, of proline-rich proteins in lamb saliva. The results suggest that the aerial parts of C. ladanifer are poor in palatability and that salivary proteins from lambs, due to their affinity for this plant’s secondary metabolites, can contribute to this. Further protein identification is needed, as well as studies to access the stability of the tannin-protein complexes through the gastrointestinal tract. |
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Changes in salivary protein composition of lambs supplemented with aerial parts and condensed tannins: extract from Cistus ladanifer L. - a preliminary study.Cistus ladanifer L.Condensed tanninsLambsSalivaTannin-binding salivary proteinsCistus ladanifer L. is a shrub present in Mediterranean areas without major use, but which can be incorporated into ruminant diets, improving the digestive efficiency of dietary protein and product quality. The high levels of plant secondary metabolites, including condensed tannins, may be responsible for the beneficial properties of the plant, but can also reduce palatability. In this study, C. ladanifer was incorporated into lambs’ diets, either as aerial parts or as a condensed tannin extract, reaching 1.25% and 2.5% of condensed tannins of dry matter. Saliva was collected and electrophoretic profiles of both whole saliva and saliva after in vitro incubation with C. ladanifer tannins were compared. Animals receiving the aerial parts of C. ladanifer decreased feed ingestion. Differences in salivary protein profiles were observed for animals fed with the higher levels of aerial parts of the plant (CL2.5 group). Most of the lambs’ salivary proteins were present in the precipitate formed after tannin-saliva incubation, and one of the bands increased in CL2.5 group was present in high proportion in the precipitate. None of the protein bands stained pink with Coomassie Brilliant Blue, suggesting the absence, or low amounts, of proline-rich proteins in lamb saliva. The results suggest that the aerial parts of C. ladanifer are poor in palatability and that salivary proteins from lambs, due to their affinity for this plant’s secondary metabolites, can contribute to this. Further protein identification is needed, as well as studies to access the stability of the tannin-protein complexes through the gastrointestinal tract.Springer2020-02-26T16:38:09Z2020-02-262019-04-12T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10174/27366http://hdl.handle.net/10174/27366https://doi.org/10.1007/s10457-019-00386-4porLamy E, Rodrigues L, Guerreiro O, Soldado D, Francisco A, Lima M, Capela e Silva F, Lopes O, Santos-Silva J, Jerónimo E. Changes in salivary protein composition of lambs supplemented with aerial parts and condensed tannins: extract from Cistus ladanifer L. - a preliminary study. Agroforest Syst (2019). DOI: 10.1007/s10457-019-00386-4https://link.springer.com/article/10.1007/s10457-019-00386-4Journal of ProteomicsICAAM (MED)ecsl@uevora.ptndndndndmopl@uevora.ptfcs@uevora.ptosl@uevora.ptndnd226Lamy, ElsaRodrigues, LeniaGurreiro, OSoldado, DAmado, FMonica, LimaCapela e Silva, F.Lopes, OrlandoSantos-Silva, JJerónimo, Einfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T19:22:49Zoai:dspace.uevora.pt:10174/27366Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:17:28.787615Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Changes in salivary protein composition of lambs supplemented with aerial parts and condensed tannins: extract from Cistus ladanifer L. - a preliminary study. |
title |
Changes in salivary protein composition of lambs supplemented with aerial parts and condensed tannins: extract from Cistus ladanifer L. - a preliminary study. |
spellingShingle |
Changes in salivary protein composition of lambs supplemented with aerial parts and condensed tannins: extract from Cistus ladanifer L. - a preliminary study. Lamy, Elsa Cistus ladanifer L. Condensed tannins Lambs Saliva Tannin-binding salivary proteins |
title_short |
Changes in salivary protein composition of lambs supplemented with aerial parts and condensed tannins: extract from Cistus ladanifer L. - a preliminary study. |
title_full |
Changes in salivary protein composition of lambs supplemented with aerial parts and condensed tannins: extract from Cistus ladanifer L. - a preliminary study. |
title_fullStr |
Changes in salivary protein composition of lambs supplemented with aerial parts and condensed tannins: extract from Cistus ladanifer L. - a preliminary study. |
title_full_unstemmed |
Changes in salivary protein composition of lambs supplemented with aerial parts and condensed tannins: extract from Cistus ladanifer L. - a preliminary study. |
title_sort |
Changes in salivary protein composition of lambs supplemented with aerial parts and condensed tannins: extract from Cistus ladanifer L. - a preliminary study. |
author |
Lamy, Elsa |
author_facet |
Lamy, Elsa Rodrigues, Lenia Gurreiro, O Soldado, D Amado, F Monica, Lima Capela e Silva, F. Lopes, Orlando Santos-Silva, J Jerónimo, E |
author_role |
author |
author2 |
Rodrigues, Lenia Gurreiro, O Soldado, D Amado, F Monica, Lima Capela e Silva, F. Lopes, Orlando Santos-Silva, J Jerónimo, E |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Lamy, Elsa Rodrigues, Lenia Gurreiro, O Soldado, D Amado, F Monica, Lima Capela e Silva, F. Lopes, Orlando Santos-Silva, J Jerónimo, E |
dc.subject.por.fl_str_mv |
Cistus ladanifer L. Condensed tannins Lambs Saliva Tannin-binding salivary proteins |
topic |
Cistus ladanifer L. Condensed tannins Lambs Saliva Tannin-binding salivary proteins |
description |
Cistus ladanifer L. is a shrub present in Mediterranean areas without major use, but which can be incorporated into ruminant diets, improving the digestive efficiency of dietary protein and product quality. The high levels of plant secondary metabolites, including condensed tannins, may be responsible for the beneficial properties of the plant, but can also reduce palatability. In this study, C. ladanifer was incorporated into lambs’ diets, either as aerial parts or as a condensed tannin extract, reaching 1.25% and 2.5% of condensed tannins of dry matter. Saliva was collected and electrophoretic profiles of both whole saliva and saliva after in vitro incubation with C. ladanifer tannins were compared. Animals receiving the aerial parts of C. ladanifer decreased feed ingestion. Differences in salivary protein profiles were observed for animals fed with the higher levels of aerial parts of the plant (CL2.5 group). Most of the lambs’ salivary proteins were present in the precipitate formed after tannin-saliva incubation, and one of the bands increased in CL2.5 group was present in high proportion in the precipitate. None of the protein bands stained pink with Coomassie Brilliant Blue, suggesting the absence, or low amounts, of proline-rich proteins in lamb saliva. The results suggest that the aerial parts of C. ladanifer are poor in palatability and that salivary proteins from lambs, due to their affinity for this plant’s secondary metabolites, can contribute to this. Further protein identification is needed, as well as studies to access the stability of the tannin-protein complexes through the gastrointestinal tract. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-04-12T00:00:00Z 2020-02-26T16:38:09Z 2020-02-26 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10174/27366 http://hdl.handle.net/10174/27366 https://doi.org/10.1007/s10457-019-00386-4 |
url |
http://hdl.handle.net/10174/27366 https://doi.org/10.1007/s10457-019-00386-4 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
Lamy E, Rodrigues L, Guerreiro O, Soldado D, Francisco A, Lima M, Capela e Silva F, Lopes O, Santos-Silva J, Jerónimo E. Changes in salivary protein composition of lambs supplemented with aerial parts and condensed tannins: extract from Cistus ladanifer L. - a preliminary study. Agroforest Syst (2019). DOI: 10.1007/s10457-019-00386-4 https://link.springer.com/article/10.1007/s10457-019-00386-4 Journal of Proteomics ICAAM (MED) ecsl@uevora.pt nd nd nd nd mopl@uevora.pt fcs@uevora.pt osl@uevora.pt nd nd 226 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799136656972840960 |