Changes in salivary protein composition of lambs supplemented with aerial parts and condensed tannins: extract from Cistus ladanifer L. - a preliminary study.

Detalhes bibliográficos
Autor(a) principal: Lamy, Elsa
Data de Publicação: 2019
Outros Autores: Rodrigues, Lenia, Gurreiro, O, Soldado, D, Amado, F, Monica, Lima, Capela e Silva, F., Lopes, Orlando, Santos-Silva, J, Jerónimo, E
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10174/27366
https://doi.org/10.1007/s10457-019-00386-4
Resumo: Cistus ladanifer L. is a shrub present in Mediterranean areas without major use, but which can be incorporated into ruminant diets, improving the digestive efficiency of dietary protein and product quality. The high levels of plant secondary metabolites, including condensed tannins, may be responsible for the beneficial properties of the plant, but can also reduce palatability. In this study, C. ladanifer was incorporated into lambs’ diets, either as aerial parts or as a condensed tannin extract, reaching 1.25% and 2.5% of condensed tannins of dry matter. Saliva was collected and electrophoretic profiles of both whole saliva and saliva after in vitro incubation with C. ladanifer tannins were compared. Animals receiving the aerial parts of C. ladanifer decreased feed ingestion. Differences in salivary protein profiles were observed for animals fed with the higher levels of aerial parts of the plant (CL2.5 group). Most of the lambs’ salivary proteins were present in the precipitate formed after tannin-saliva incubation, and one of the bands increased in CL2.5 group was present in high proportion in the precipitate. None of the protein bands stained pink with Coomassie Brilliant Blue, suggesting the absence, or low amounts, of proline-rich proteins in lamb saliva. The results suggest that the aerial parts of C. ladanifer are poor in palatability and that salivary proteins from lambs, due to their affinity for this plant’s secondary metabolites, can contribute to this. Further protein identification is needed, as well as studies to access the stability of the tannin-protein complexes through the gastrointestinal tract.
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spelling Changes in salivary protein composition of lambs supplemented with aerial parts and condensed tannins: extract from Cistus ladanifer L. - a preliminary study.Cistus ladanifer L.Condensed tanninsLambsSalivaTannin-binding salivary proteinsCistus ladanifer L. is a shrub present in Mediterranean areas without major use, but which can be incorporated into ruminant diets, improving the digestive efficiency of dietary protein and product quality. The high levels of plant secondary metabolites, including condensed tannins, may be responsible for the beneficial properties of the plant, but can also reduce palatability. In this study, C. ladanifer was incorporated into lambs’ diets, either as aerial parts or as a condensed tannin extract, reaching 1.25% and 2.5% of condensed tannins of dry matter. Saliva was collected and electrophoretic profiles of both whole saliva and saliva after in vitro incubation with C. ladanifer tannins were compared. Animals receiving the aerial parts of C. ladanifer decreased feed ingestion. Differences in salivary protein profiles were observed for animals fed with the higher levels of aerial parts of the plant (CL2.5 group). Most of the lambs’ salivary proteins were present in the precipitate formed after tannin-saliva incubation, and one of the bands increased in CL2.5 group was present in high proportion in the precipitate. None of the protein bands stained pink with Coomassie Brilliant Blue, suggesting the absence, or low amounts, of proline-rich proteins in lamb saliva. The results suggest that the aerial parts of C. ladanifer are poor in palatability and that salivary proteins from lambs, due to their affinity for this plant’s secondary metabolites, can contribute to this. Further protein identification is needed, as well as studies to access the stability of the tannin-protein complexes through the gastrointestinal tract.Springer2020-02-26T16:38:09Z2020-02-262019-04-12T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10174/27366http://hdl.handle.net/10174/27366https://doi.org/10.1007/s10457-019-00386-4porLamy E, Rodrigues L, Guerreiro O, Soldado D, Francisco A, Lima M, Capela e Silva F, Lopes O, Santos-Silva J, Jerónimo E. Changes in salivary protein composition of lambs supplemented with aerial parts and condensed tannins: extract from Cistus ladanifer L. - a preliminary study. Agroforest Syst (2019). DOI: 10.1007/s10457-019-00386-4https://link.springer.com/article/10.1007/s10457-019-00386-4Journal of ProteomicsICAAM (MED)ecsl@uevora.ptndndndndmopl@uevora.ptfcs@uevora.ptosl@uevora.ptndnd226Lamy, ElsaRodrigues, LeniaGurreiro, OSoldado, DAmado, FMonica, LimaCapela e Silva, F.Lopes, OrlandoSantos-Silva, JJerónimo, Einfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T19:22:49Zoai:dspace.uevora.pt:10174/27366Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:17:28.787615Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Changes in salivary protein composition of lambs supplemented with aerial parts and condensed tannins: extract from Cistus ladanifer L. - a preliminary study.
title Changes in salivary protein composition of lambs supplemented with aerial parts and condensed tannins: extract from Cistus ladanifer L. - a preliminary study.
spellingShingle Changes in salivary protein composition of lambs supplemented with aerial parts and condensed tannins: extract from Cistus ladanifer L. - a preliminary study.
Lamy, Elsa
Cistus ladanifer L.
Condensed tannins
Lambs
Saliva
Tannin-binding salivary proteins
title_short Changes in salivary protein composition of lambs supplemented with aerial parts and condensed tannins: extract from Cistus ladanifer L. - a preliminary study.
title_full Changes in salivary protein composition of lambs supplemented with aerial parts and condensed tannins: extract from Cistus ladanifer L. - a preliminary study.
title_fullStr Changes in salivary protein composition of lambs supplemented with aerial parts and condensed tannins: extract from Cistus ladanifer L. - a preliminary study.
title_full_unstemmed Changes in salivary protein composition of lambs supplemented with aerial parts and condensed tannins: extract from Cistus ladanifer L. - a preliminary study.
title_sort Changes in salivary protein composition of lambs supplemented with aerial parts and condensed tannins: extract from Cistus ladanifer L. - a preliminary study.
author Lamy, Elsa
author_facet Lamy, Elsa
Rodrigues, Lenia
Gurreiro, O
Soldado, D
Amado, F
Monica, Lima
Capela e Silva, F.
Lopes, Orlando
Santos-Silva, J
Jerónimo, E
author_role author
author2 Rodrigues, Lenia
Gurreiro, O
Soldado, D
Amado, F
Monica, Lima
Capela e Silva, F.
Lopes, Orlando
Santos-Silva, J
Jerónimo, E
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Lamy, Elsa
Rodrigues, Lenia
Gurreiro, O
Soldado, D
Amado, F
Monica, Lima
Capela e Silva, F.
Lopes, Orlando
Santos-Silva, J
Jerónimo, E
dc.subject.por.fl_str_mv Cistus ladanifer L.
Condensed tannins
Lambs
Saliva
Tannin-binding salivary proteins
topic Cistus ladanifer L.
Condensed tannins
Lambs
Saliva
Tannin-binding salivary proteins
description Cistus ladanifer L. is a shrub present in Mediterranean areas without major use, but which can be incorporated into ruminant diets, improving the digestive efficiency of dietary protein and product quality. The high levels of plant secondary metabolites, including condensed tannins, may be responsible for the beneficial properties of the plant, but can also reduce palatability. In this study, C. ladanifer was incorporated into lambs’ diets, either as aerial parts or as a condensed tannin extract, reaching 1.25% and 2.5% of condensed tannins of dry matter. Saliva was collected and electrophoretic profiles of both whole saliva and saliva after in vitro incubation with C. ladanifer tannins were compared. Animals receiving the aerial parts of C. ladanifer decreased feed ingestion. Differences in salivary protein profiles were observed for animals fed with the higher levels of aerial parts of the plant (CL2.5 group). Most of the lambs’ salivary proteins were present in the precipitate formed after tannin-saliva incubation, and one of the bands increased in CL2.5 group was present in high proportion in the precipitate. None of the protein bands stained pink with Coomassie Brilliant Blue, suggesting the absence, or low amounts, of proline-rich proteins in lamb saliva. The results suggest that the aerial parts of C. ladanifer are poor in palatability and that salivary proteins from lambs, due to their affinity for this plant’s secondary metabolites, can contribute to this. Further protein identification is needed, as well as studies to access the stability of the tannin-protein complexes through the gastrointestinal tract.
publishDate 2019
dc.date.none.fl_str_mv 2019-04-12T00:00:00Z
2020-02-26T16:38:09Z
2020-02-26
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10174/27366
http://hdl.handle.net/10174/27366
https://doi.org/10.1007/s10457-019-00386-4
url http://hdl.handle.net/10174/27366
https://doi.org/10.1007/s10457-019-00386-4
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv Lamy E, Rodrigues L, Guerreiro O, Soldado D, Francisco A, Lima M, Capela e Silva F, Lopes O, Santos-Silva J, Jerónimo E. Changes in salivary protein composition of lambs supplemented with aerial parts and condensed tannins: extract from Cistus ladanifer L. - a preliminary study. Agroforest Syst (2019). DOI: 10.1007/s10457-019-00386-4
https://link.springer.com/article/10.1007/s10457-019-00386-4
Journal of Proteomics
ICAAM (MED)
ecsl@uevora.pt
nd
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nd
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mopl@uevora.pt
fcs@uevora.pt
osl@uevora.pt
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dc.publisher.none.fl_str_mv Springer
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