The Influence of Solvent and Extraction Time on Yield and Chemical Selectivity of Cuticular Waxes from Quercus suber Leaves
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.5/26225 |
Resumo: | The cuticular lipid compounds, usually named cuticular waxes, present in the cuticular layering of Quercus suber adult leaves were extracted with solvents of different polarities (n-hexane, dichloromethane and acetone) and analysed by GC–MS. Q. suber leaves have a substantial cuticular wax layer (2.8% of leaf mass and 239 g/cm2), composed predominantly by terpenes (43–63% of all compounds), followed by aliphatic long chain molecules, mainly fatty acids, and by smaller amounts of aliphatic alcohols and n-alkanes. The major identified compound was lupeol (1.2% of leaves in n-hexane extract). The recovery and composition of cuticular lipids depended on the solvent and extraction time. The non-polar or weak polar solvents n-hexane and dichloromethane extracted similar lipid yields (77% and 86% of the total extract, respectively) while acetone solubilised other cellular compounds, namely sugars, with the lipid compounds representing 43% of the total extract. For cuticular lipids extraction, solvents with a low polarity such as n-hexane are the more suitable with an adequate extraction duration, e.g., n-hexane with a minimum extraction of 3 h |
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The Influence of Solvent and Extraction Time on Yield and Chemical Selectivity of Cuticular Waxes from Quercus suber Leavescork oakcuticular waxsolventsterpeneslupeolThe cuticular lipid compounds, usually named cuticular waxes, present in the cuticular layering of Quercus suber adult leaves were extracted with solvents of different polarities (n-hexane, dichloromethane and acetone) and analysed by GC–MS. Q. suber leaves have a substantial cuticular wax layer (2.8% of leaf mass and 239 g/cm2), composed predominantly by terpenes (43–63% of all compounds), followed by aliphatic long chain molecules, mainly fatty acids, and by smaller amounts of aliphatic alcohols and n-alkanes. The major identified compound was lupeol (1.2% of leaves in n-hexane extract). The recovery and composition of cuticular lipids depended on the solvent and extraction time. The non-polar or weak polar solvents n-hexane and dichloromethane extracted similar lipid yields (77% and 86% of the total extract, respectively) while acetone solubilised other cellular compounds, namely sugars, with the lipid compounds representing 43% of the total extract. For cuticular lipids extraction, solvents with a low polarity such as n-hexane are the more suitable with an adequate extraction duration, e.g., n-hexane with a minimum extraction of 3 hMDPIRepositório da Universidade de LisboaSimões, RitaMiranda, IsabelPereira, H.2022-11-25T11:45:16Z20222022-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/26225engSimões, R.; Miranda, I.; Pereira, H. The Influence of Solvent and Extraction Time on Yield and Chemical Selectivity of Cuticular Waxes from Quercus suber Leaves. Processes 2022, 10, 2270https:// doi.org/10.3390/pr10112270info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:55:42Zoai:www.repository.utl.pt:10400.5/26225Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:09:55.197580Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
The Influence of Solvent and Extraction Time on Yield and Chemical Selectivity of Cuticular Waxes from Quercus suber Leaves |
title |
The Influence of Solvent and Extraction Time on Yield and Chemical Selectivity of Cuticular Waxes from Quercus suber Leaves |
spellingShingle |
The Influence of Solvent and Extraction Time on Yield and Chemical Selectivity of Cuticular Waxes from Quercus suber Leaves Simões, Rita cork oak cuticular wax solvents terpenes lupeol |
title_short |
The Influence of Solvent and Extraction Time on Yield and Chemical Selectivity of Cuticular Waxes from Quercus suber Leaves |
title_full |
The Influence of Solvent and Extraction Time on Yield and Chemical Selectivity of Cuticular Waxes from Quercus suber Leaves |
title_fullStr |
The Influence of Solvent and Extraction Time on Yield and Chemical Selectivity of Cuticular Waxes from Quercus suber Leaves |
title_full_unstemmed |
The Influence of Solvent and Extraction Time on Yield and Chemical Selectivity of Cuticular Waxes from Quercus suber Leaves |
title_sort |
The Influence of Solvent and Extraction Time on Yield and Chemical Selectivity of Cuticular Waxes from Quercus suber Leaves |
author |
Simões, Rita |
author_facet |
Simões, Rita Miranda, Isabel Pereira, H. |
author_role |
author |
author2 |
Miranda, Isabel Pereira, H. |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
dc.contributor.author.fl_str_mv |
Simões, Rita Miranda, Isabel Pereira, H. |
dc.subject.por.fl_str_mv |
cork oak cuticular wax solvents terpenes lupeol |
topic |
cork oak cuticular wax solvents terpenes lupeol |
description |
The cuticular lipid compounds, usually named cuticular waxes, present in the cuticular layering of Quercus suber adult leaves were extracted with solvents of different polarities (n-hexane, dichloromethane and acetone) and analysed by GC–MS. Q. suber leaves have a substantial cuticular wax layer (2.8% of leaf mass and 239 g/cm2), composed predominantly by terpenes (43–63% of all compounds), followed by aliphatic long chain molecules, mainly fatty acids, and by smaller amounts of aliphatic alcohols and n-alkanes. The major identified compound was lupeol (1.2% of leaves in n-hexane extract). The recovery and composition of cuticular lipids depended on the solvent and extraction time. The non-polar or weak polar solvents n-hexane and dichloromethane extracted similar lipid yields (77% and 86% of the total extract, respectively) while acetone solubilised other cellular compounds, namely sugars, with the lipid compounds representing 43% of the total extract. For cuticular lipids extraction, solvents with a low polarity such as n-hexane are the more suitable with an adequate extraction duration, e.g., n-hexane with a minimum extraction of 3 h |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-11-25T11:45:16Z 2022 2022-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.5/26225 |
url |
http://hdl.handle.net/10400.5/26225 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Simões, R.; Miranda, I.; Pereira, H. The Influence of Solvent and Extraction Time on Yield and Chemical Selectivity of Cuticular Waxes from Quercus suber Leaves. Processes 2022, 10, 2270 https:// doi.org/10.3390/pr10112270 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799131194919485440 |