Gastric cancer extracellular vesicles tune the migration and invasion of epithelial and mesenchymal cells in a histotype-dependent manner

Detalhes bibliográficos
Autor(a) principal: Rocha, S
Data de Publicação: 2019
Outros Autores: Teles, SP, Azevedo, M, Oliveira, P, Carvalho, J, Oliveira, C
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://hdl.handle.net/10216/136348
Resumo: Extracellular vesicles (EVs) secreted by tumor cells modulate recipient cells’ behavior, but their effects in normal cells from the tumor microenvironment remain poorly known. In this study, we dissected the functional impact of gastric cancer cell-derived EVs (GC-EVs), representative of distinct GC histotypes, on the behavior of normal isogenic epithelial and mesenchymal cells. GC-EVs were isolated by differential centrifugation and characterized by transmission electron microscopy, nanoparticle tracking analysis, and imaging flow-cytometry. Epithelial and mesenchymal cells were challenged with GC-EVs and submitted to proliferation, migration, and invasion assays. Expression of epithelial and mesenchymal markers was followed by immunofluorescence and flow-cytometry. Our results indicated that GC-EVs secreted by diffuse-type cancer cells decrease the migration of recipient cells. This effect was more prominent and persistent for mesenchymal recipient cells, which also increased Fibronectin expression in response to EVs. GC-EVs secreted by cancer cells derived from tumors with an intestinal component increased invasion of recipient epithelial cells, without changes in EMT markers. In summary, this study demonstrated that GC-EVs modulate the migration and invasion of epithelial and mesenchymal cells from the tumor microenvironment, in a histotype-dependent manner, highlighting new features of intestinal and diffuse-type GC cells, which may help explaining differential metastasis patterns and aggressiveness of GC histotypes.
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spelling Gastric cancer extracellular vesicles tune the migration and invasion of epithelial and mesenchymal cells in a histotype-dependent mannerEpithelial-to-mesenchymal transitionExtracellular vesiclesGastric cancerInvasionMigrationExtracellular vesicles (EVs) secreted by tumor cells modulate recipient cells’ behavior, but their effects in normal cells from the tumor microenvironment remain poorly known. In this study, we dissected the functional impact of gastric cancer cell-derived EVs (GC-EVs), representative of distinct GC histotypes, on the behavior of normal isogenic epithelial and mesenchymal cells. GC-EVs were isolated by differential centrifugation and characterized by transmission electron microscopy, nanoparticle tracking analysis, and imaging flow-cytometry. Epithelial and mesenchymal cells were challenged with GC-EVs and submitted to proliferation, migration, and invasion assays. Expression of epithelial and mesenchymal markers was followed by immunofluorescence and flow-cytometry. Our results indicated that GC-EVs secreted by diffuse-type cancer cells decrease the migration of recipient cells. This effect was more prominent and persistent for mesenchymal recipient cells, which also increased Fibronectin expression in response to EVs. GC-EVs secreted by cancer cells derived from tumors with an intestinal component increased invasion of recipient epithelial cells, without changes in EMT markers. In summary, this study demonstrated that GC-EVs modulate the migration and invasion of epithelial and mesenchymal cells from the tumor microenvironment, in a histotype-dependent manner, highlighting new features of intestinal and diffuse-type GC cells, which may help explaining differential metastasis patterns and aggressiveness of GC histotypes.MDPI20192019-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10216/136348eng1661-659610.3390/ijms20112608Rocha, STeles, SPAzevedo, MOliveira, PCarvalho, JOliveira, Cinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-29T12:57:47Zoai:repositorio-aberto.up.pt:10216/136348Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T23:30:31.189494Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Gastric cancer extracellular vesicles tune the migration and invasion of epithelial and mesenchymal cells in a histotype-dependent manner
title Gastric cancer extracellular vesicles tune the migration and invasion of epithelial and mesenchymal cells in a histotype-dependent manner
spellingShingle Gastric cancer extracellular vesicles tune the migration and invasion of epithelial and mesenchymal cells in a histotype-dependent manner
Rocha, S
Epithelial-to-mesenchymal transition
Extracellular vesicles
Gastric cancer
Invasion
Migration
title_short Gastric cancer extracellular vesicles tune the migration and invasion of epithelial and mesenchymal cells in a histotype-dependent manner
title_full Gastric cancer extracellular vesicles tune the migration and invasion of epithelial and mesenchymal cells in a histotype-dependent manner
title_fullStr Gastric cancer extracellular vesicles tune the migration and invasion of epithelial and mesenchymal cells in a histotype-dependent manner
title_full_unstemmed Gastric cancer extracellular vesicles tune the migration and invasion of epithelial and mesenchymal cells in a histotype-dependent manner
title_sort Gastric cancer extracellular vesicles tune the migration and invasion of epithelial and mesenchymal cells in a histotype-dependent manner
author Rocha, S
author_facet Rocha, S
Teles, SP
Azevedo, M
Oliveira, P
Carvalho, J
Oliveira, C
author_role author
author2 Teles, SP
Azevedo, M
Oliveira, P
Carvalho, J
Oliveira, C
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Rocha, S
Teles, SP
Azevedo, M
Oliveira, P
Carvalho, J
Oliveira, C
dc.subject.por.fl_str_mv Epithelial-to-mesenchymal transition
Extracellular vesicles
Gastric cancer
Invasion
Migration
topic Epithelial-to-mesenchymal transition
Extracellular vesicles
Gastric cancer
Invasion
Migration
description Extracellular vesicles (EVs) secreted by tumor cells modulate recipient cells’ behavior, but their effects in normal cells from the tumor microenvironment remain poorly known. In this study, we dissected the functional impact of gastric cancer cell-derived EVs (GC-EVs), representative of distinct GC histotypes, on the behavior of normal isogenic epithelial and mesenchymal cells. GC-EVs were isolated by differential centrifugation and characterized by transmission electron microscopy, nanoparticle tracking analysis, and imaging flow-cytometry. Epithelial and mesenchymal cells were challenged with GC-EVs and submitted to proliferation, migration, and invasion assays. Expression of epithelial and mesenchymal markers was followed by immunofluorescence and flow-cytometry. Our results indicated that GC-EVs secreted by diffuse-type cancer cells decrease the migration of recipient cells. This effect was more prominent and persistent for mesenchymal recipient cells, which also increased Fibronectin expression in response to EVs. GC-EVs secreted by cancer cells derived from tumors with an intestinal component increased invasion of recipient epithelial cells, without changes in EMT markers. In summary, this study demonstrated that GC-EVs modulate the migration and invasion of epithelial and mesenchymal cells from the tumor microenvironment, in a histotype-dependent manner, highlighting new features of intestinal and diffuse-type GC cells, which may help explaining differential metastasis patterns and aggressiveness of GC histotypes.
publishDate 2019
dc.date.none.fl_str_mv 2019
2019-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.uri.fl_str_mv https://hdl.handle.net/10216/136348
url https://hdl.handle.net/10216/136348
dc.language.iso.fl_str_mv eng
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dc.relation.none.fl_str_mv 1661-6596
10.3390/ijms20112608
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instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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