Notable differences in gene expression between injured turbot (Scophthalmus maximus) and brill (Scophthalmus rhombus) skin

Detalhes bibliográficos
Autor(a) principal: Estêvão, João Luis Correia
Data de Publicação: 2015
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.1/7704
Resumo: Dissertação de mestrado, Engenharia Biológica, Faculdade de Ciências e Tecnologia, Universidade do Algarve, 2015
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spelling Notable differences in gene expression between injured turbot (Scophthalmus maximus) and brill (Scophthalmus rhombus) skinEngenharia biológicaPeixesLesões da pelePregadoRodovalhoImunidade inataVariações genéticasDomínio/Área Científica::Ciências Naturais::Ciências BiológicasDissertação de mestrado, Engenharia Biológica, Faculdade de Ciências e Tecnologia, Universidade do Algarve, 2015The skin, the largest organ of fish’s body and first barrier against external agents, plays a crucial role against environmental aggressions and is decisive in communication between individuals. Cutaneous lesions are common in fish and may result in an open door for infectious agents and originate osmotic stress, which can be life threatening for the animal. Turbot (Scophthalmus maximus) and brill (Scophthalmus rhombus) are two closely related congeneric species with striking differences in their skin, since turbot presents tubercles instead of the scales found in brill. In this work, gene expression analyses were performed in order to analyze the genetic differences in the skin response between the two species. Skin scraping areas (72 h after injury) from three biological replicates of each species were compared with normal skin areas from the same individuals. A previously reported 4x44k Agilent turbot microarray was employed, after evaluating its suitability by comparison of the base sequence of common genes using bioinformatics. Skin-related genes from previous studies (1564 sequences) were taken as a reference to screen the turbot database and the oligo-probes in the microarray. Among them, 584 (~37%) were present in the turbot database (e-value < 9x10-20) and 326 (~21%) contained oligo-probe in the microarray, a notable result since the microarray was especially enriched with immune-related organs. Genes with a significant fluorescent signal in the microarray (>200 fluorescence units) were identified in each (turbot: 15,739; brill: 12,393) and in both species (10,065). Among them, 1750 differentially expressed genes (t-test p-value p<0.005; log2 ratio <-2 and >2) were detected in brill, 1461 in turbot, and 885 showed differences between species, respectively. These results strongly suggest: 1) the turbot microarray is suitable for gene expression analysis in brill, and 2) a notable different occurs in the response of the skin to damage in the two species.Portela, Paulino MartinezPower, DeborahSapientiaEstêvão, João Luis Correia2017-02-17T01:30:09Z2015-12-1520152015-12-15T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfapplication/vnd.openxmlformats-officedocument.spreadsheetml.sheetapplication/vnd.openxmlformats-officedocument.spreadsheetml.sheetapplication/vnd.openxmlformats-officedocument.spreadsheetml.sheetapplication/vnd.openxmlformats-officedocument.spreadsheetml.sheethttp://hdl.handle.net/10400.1/7704TID:201179695enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-24T10:18:51Zoai:sapientia.ualg.pt:10400.1/7704Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:00:00.821882Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Notable differences in gene expression between injured turbot (Scophthalmus maximus) and brill (Scophthalmus rhombus) skin
title Notable differences in gene expression between injured turbot (Scophthalmus maximus) and brill (Scophthalmus rhombus) skin
spellingShingle Notable differences in gene expression between injured turbot (Scophthalmus maximus) and brill (Scophthalmus rhombus) skin
Estêvão, João Luis Correia
Engenharia biológica
Peixes
Lesões da pele
Pregado
Rodovalho
Imunidade inata
Variações genéticas
Domínio/Área Científica::Ciências Naturais::Ciências Biológicas
title_short Notable differences in gene expression between injured turbot (Scophthalmus maximus) and brill (Scophthalmus rhombus) skin
title_full Notable differences in gene expression between injured turbot (Scophthalmus maximus) and brill (Scophthalmus rhombus) skin
title_fullStr Notable differences in gene expression between injured turbot (Scophthalmus maximus) and brill (Scophthalmus rhombus) skin
title_full_unstemmed Notable differences in gene expression between injured turbot (Scophthalmus maximus) and brill (Scophthalmus rhombus) skin
title_sort Notable differences in gene expression between injured turbot (Scophthalmus maximus) and brill (Scophthalmus rhombus) skin
author Estêvão, João Luis Correia
author_facet Estêvão, João Luis Correia
author_role author
dc.contributor.none.fl_str_mv Portela, Paulino Martinez
Power, Deborah
Sapientia
dc.contributor.author.fl_str_mv Estêvão, João Luis Correia
dc.subject.por.fl_str_mv Engenharia biológica
Peixes
Lesões da pele
Pregado
Rodovalho
Imunidade inata
Variações genéticas
Domínio/Área Científica::Ciências Naturais::Ciências Biológicas
topic Engenharia biológica
Peixes
Lesões da pele
Pregado
Rodovalho
Imunidade inata
Variações genéticas
Domínio/Área Científica::Ciências Naturais::Ciências Biológicas
description Dissertação de mestrado, Engenharia Biológica, Faculdade de Ciências e Tecnologia, Universidade do Algarve, 2015
publishDate 2015
dc.date.none.fl_str_mv 2015-12-15
2015
2015-12-15T00:00:00Z
2017-02-17T01:30:09Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.1/7704
TID:201179695
url http://hdl.handle.net/10400.1/7704
identifier_str_mv TID:201179695
dc.language.iso.fl_str_mv eng
language eng
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eu_rights_str_mv openAccess
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dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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