Exploring freeze-drying as strategy to enhance viability of faecalibacterium duncaniae dsm 17677 upon aerobic storage and gastrointestinal conditions
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.14/39633 |
Resumo: | Faecalibacterium duncaniae is an intestinal commensal bacterium proposed as a next-generation probiotic due to its promising outcomes in the treatment and prevention of several human diseases, which demonstrate its multiple contributions to the host’s health. However, its strict anaerobic nature has created several hurdles in the development of functional foods, nutraceuticals, and biotherapeutic products. Herein, we explored freeze-dried formulations containing prebiotics, cryoprotectants, and antioxidant agents as a technological strategy to enhance the viability of F. duncaniae DSM 17677 upon aerobic storage and gastrointestinal tract conditions. Our results indicate that freeze-dried F. duncaniae in a matrix containing inulin, sucrose, cysteine, and riboflavin survived at levels higher than 106 CFU/g and around 105 CFU/g after 1 and 4 days of aerobic storage at room temperature, respectively. Thus, the freeze-dried formulation with inulin, sucrose, cysteine, and riboflavin presents as a protective strategy to improve F. duncaniae viability under aerobic environments. Nevertheless, incorporation of a suitable coating aimed at protecting F. duncaniae against the detrimental gastrointestinal passage effects is urgently required, given its high susceptibility to extreme acidic pH values and bile. |
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Exploring freeze-drying as strategy to enhance viability of faecalibacterium duncaniae dsm 17677 upon aerobic storage and gastrointestinal conditionsAcidic pH susceptibilityAerobic storageAntioxidantsBile susceptibilityCryoprotectantsFaecalibacterium duncaniaeFreeze-dryingGastrointestinal conditionsNext-generation probioticsViabilityFaecalibacterium duncaniae is an intestinal commensal bacterium proposed as a next-generation probiotic due to its promising outcomes in the treatment and prevention of several human diseases, which demonstrate its multiple contributions to the host’s health. However, its strict anaerobic nature has created several hurdles in the development of functional foods, nutraceuticals, and biotherapeutic products. Herein, we explored freeze-dried formulations containing prebiotics, cryoprotectants, and antioxidant agents as a technological strategy to enhance the viability of F. duncaniae DSM 17677 upon aerobic storage and gastrointestinal tract conditions. Our results indicate that freeze-dried F. duncaniae in a matrix containing inulin, sucrose, cysteine, and riboflavin survived at levels higher than 106 CFU/g and around 105 CFU/g after 1 and 4 days of aerobic storage at room temperature, respectively. Thus, the freeze-dried formulation with inulin, sucrose, cysteine, and riboflavin presents as a protective strategy to improve F. duncaniae viability under aerobic environments. Nevertheless, incorporation of a suitable coating aimed at protecting F. duncaniae against the detrimental gastrointestinal passage effects is urgently required, given its high susceptibility to extreme acidic pH values and bile.Veritati - Repositório Institucional da Universidade Católica PortuguesaMachado, DanielaDomingos, MelanyBarbosa, Joana CristinaAlmeida, DianaAndrade, José CarlosFreitas, Ana CristinaGomes, Ana Maria2022-12-21T18:03:43Z2022-12-072022-12-07T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.14/39633eng1999-492310.3390/pharmaceutics1412273585144846100PMC978561136559229000904187600001info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-09-06T12:40:03Zoai:repositorio.ucp.pt:10400.14/39633Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-09-06T12:40:03Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Exploring freeze-drying as strategy to enhance viability of faecalibacterium duncaniae dsm 17677 upon aerobic storage and gastrointestinal conditions |
title |
Exploring freeze-drying as strategy to enhance viability of faecalibacterium duncaniae dsm 17677 upon aerobic storage and gastrointestinal conditions |
spellingShingle |
Exploring freeze-drying as strategy to enhance viability of faecalibacterium duncaniae dsm 17677 upon aerobic storage and gastrointestinal conditions Machado, Daniela Acidic pH susceptibility Aerobic storage Antioxidants Bile susceptibility Cryoprotectants Faecalibacterium duncaniae Freeze-drying Gastrointestinal conditions Next-generation probiotics Viability |
title_short |
Exploring freeze-drying as strategy to enhance viability of faecalibacterium duncaniae dsm 17677 upon aerobic storage and gastrointestinal conditions |
title_full |
Exploring freeze-drying as strategy to enhance viability of faecalibacterium duncaniae dsm 17677 upon aerobic storage and gastrointestinal conditions |
title_fullStr |
Exploring freeze-drying as strategy to enhance viability of faecalibacterium duncaniae dsm 17677 upon aerobic storage and gastrointestinal conditions |
title_full_unstemmed |
Exploring freeze-drying as strategy to enhance viability of faecalibacterium duncaniae dsm 17677 upon aerobic storage and gastrointestinal conditions |
title_sort |
Exploring freeze-drying as strategy to enhance viability of faecalibacterium duncaniae dsm 17677 upon aerobic storage and gastrointestinal conditions |
author |
Machado, Daniela |
author_facet |
Machado, Daniela Domingos, Melany Barbosa, Joana Cristina Almeida, Diana Andrade, José Carlos Freitas, Ana Cristina Gomes, Ana Maria |
author_role |
author |
author2 |
Domingos, Melany Barbosa, Joana Cristina Almeida, Diana Andrade, José Carlos Freitas, Ana Cristina Gomes, Ana Maria |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Veritati - Repositório Institucional da Universidade Católica Portuguesa |
dc.contributor.author.fl_str_mv |
Machado, Daniela Domingos, Melany Barbosa, Joana Cristina Almeida, Diana Andrade, José Carlos Freitas, Ana Cristina Gomes, Ana Maria |
dc.subject.por.fl_str_mv |
Acidic pH susceptibility Aerobic storage Antioxidants Bile susceptibility Cryoprotectants Faecalibacterium duncaniae Freeze-drying Gastrointestinal conditions Next-generation probiotics Viability |
topic |
Acidic pH susceptibility Aerobic storage Antioxidants Bile susceptibility Cryoprotectants Faecalibacterium duncaniae Freeze-drying Gastrointestinal conditions Next-generation probiotics Viability |
description |
Faecalibacterium duncaniae is an intestinal commensal bacterium proposed as a next-generation probiotic due to its promising outcomes in the treatment and prevention of several human diseases, which demonstrate its multiple contributions to the host’s health. However, its strict anaerobic nature has created several hurdles in the development of functional foods, nutraceuticals, and biotherapeutic products. Herein, we explored freeze-dried formulations containing prebiotics, cryoprotectants, and antioxidant agents as a technological strategy to enhance the viability of F. duncaniae DSM 17677 upon aerobic storage and gastrointestinal tract conditions. Our results indicate that freeze-dried F. duncaniae in a matrix containing inulin, sucrose, cysteine, and riboflavin survived at levels higher than 106 CFU/g and around 105 CFU/g after 1 and 4 days of aerobic storage at room temperature, respectively. Thus, the freeze-dried formulation with inulin, sucrose, cysteine, and riboflavin presents as a protective strategy to improve F. duncaniae viability under aerobic environments. Nevertheless, incorporation of a suitable coating aimed at protecting F. duncaniae against the detrimental gastrointestinal passage effects is urgently required, given its high susceptibility to extreme acidic pH values and bile. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-12-21T18:03:43Z 2022-12-07 2022-12-07T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.14/39633 |
url |
http://hdl.handle.net/10400.14/39633 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1999-4923 10.3390/pharmaceutics14122735 85144846100 PMC9785611 36559229 000904187600001 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
mluisa.alvim@gmail.com |
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1817547069971234816 |