Interaction between atypical microorganisms and E. coli in catheter-associated urinary tract biofilms
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/31858 |
Resumo: | Most biofilms involved in catheter-associated urinary tract infections (CAUTIs) are polymicrobial, with disease causing (eg Escherichia coli) and atypical microorganisms (eg Delftia tsuruhatensis) frequently inhabiting the same catheter. Nevertheless, there is a lack of knowledge about the role of atypical microorganisms. Here, single and dual-species biofilms consisting of E. coli and atypical bacteria (D. tsuruhatensis and Achromobacter xylosoxidans), were evaluated. All species were good biofilm producers (Log 5.84–7.25 CFU cm−2 at 192 h) in artificial urine. The ability of atypical species to form a biofilm appeared to be hampered by the presence of E. coli. Additionally, when E. coli was added to a pre-formed biofilm of the atypical species, it seemed to take advantage of the first colonizers to accelerate adhesion, even when added at lower concentrations. The results suggest a greater ability of E. coli to form biofilms in conditions mimicking the CAUTIs, whatever the pre-existing microbiota and the inoculum concentration. |
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Interaction between atypical microorganisms and E. coli in catheter-associated urinary tract biofilmsEscherichia coliAtypical speciesMultispecies biofilmsUrinary tract infectionsUrinary cathetersScience & TechnologyMost biofilms involved in catheter-associated urinary tract infections (CAUTIs) are polymicrobial, with disease causing (eg Escherichia coli) and atypical microorganisms (eg Delftia tsuruhatensis) frequently inhabiting the same catheter. Nevertheless, there is a lack of knowledge about the role of atypical microorganisms. Here, single and dual-species biofilms consisting of E. coli and atypical bacteria (D. tsuruhatensis and Achromobacter xylosoxidans), were evaluated. All species were good biofilm producers (Log 5.84–7.25 CFU cm−2 at 192 h) in artificial urine. The ability of atypical species to form a biofilm appeared to be hampered by the presence of E. coli. Additionally, when E. coli was added to a pre-formed biofilm of the atypical species, it seemed to take advantage of the first colonizers to accelerate adhesion, even when added at lower concentrations. The results suggest a greater ability of E. coli to form biofilms in conditions mimicking the CAUTIs, whatever the pre-existing microbiota and the inoculum concentration.This work was supported by the Portuguese Science Foundation (FCT), DNA mimics Research Project [Ref. PIC/IC/82815/2007] from the FCT and MCTES; PhD Fellowship [SFRH/BD/82663/2011]; and Postdoctoral Fellowship [SFRH/BPD/74480/2010]. The authors would like to thank to M. Fenice M and A. Steinbuchel for kindly providing the Delftia tsuruhatensis BM90 and Achromobacter xylosoxidans B3 species, respectively.Taylor and FrancisUniversidade do MinhoAzevedo, AndreiaAlmeida, CarinaMelo, L. F.Azevedo, N. F.20142014-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/31858engAzevedo, A. S.; Almeida, Carina; Melo, L. F.; Azevedo, N. F., Interaction between atypical microorganisms and E. coli in catheter-associated urinary tract biofilms. Biofouling: The Journal of Bioadhesion and Biofilm Research, 30(8), 893-902, 20140892-70141029-245410.1080/08927014.2014.94417325184430info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:50:03Zoai:repositorium.sdum.uminho.pt:1822/31858Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:48:41.566904Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Interaction between atypical microorganisms and E. coli in catheter-associated urinary tract biofilms |
title |
Interaction between atypical microorganisms and E. coli in catheter-associated urinary tract biofilms |
spellingShingle |
Interaction between atypical microorganisms and E. coli in catheter-associated urinary tract biofilms Azevedo, Andreia Escherichia coli Atypical species Multispecies biofilms Urinary tract infections Urinary catheters Science & Technology |
title_short |
Interaction between atypical microorganisms and E. coli in catheter-associated urinary tract biofilms |
title_full |
Interaction between atypical microorganisms and E. coli in catheter-associated urinary tract biofilms |
title_fullStr |
Interaction between atypical microorganisms and E. coli in catheter-associated urinary tract biofilms |
title_full_unstemmed |
Interaction between atypical microorganisms and E. coli in catheter-associated urinary tract biofilms |
title_sort |
Interaction between atypical microorganisms and E. coli in catheter-associated urinary tract biofilms |
author |
Azevedo, Andreia |
author_facet |
Azevedo, Andreia Almeida, Carina Melo, L. F. Azevedo, N. F. |
author_role |
author |
author2 |
Almeida, Carina Melo, L. F. Azevedo, N. F. |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Azevedo, Andreia Almeida, Carina Melo, L. F. Azevedo, N. F. |
dc.subject.por.fl_str_mv |
Escherichia coli Atypical species Multispecies biofilms Urinary tract infections Urinary catheters Science & Technology |
topic |
Escherichia coli Atypical species Multispecies biofilms Urinary tract infections Urinary catheters Science & Technology |
description |
Most biofilms involved in catheter-associated urinary tract infections (CAUTIs) are polymicrobial, with disease causing (eg Escherichia coli) and atypical microorganisms (eg Delftia tsuruhatensis) frequently inhabiting the same catheter. Nevertheless, there is a lack of knowledge about the role of atypical microorganisms. Here, single and dual-species biofilms consisting of E. coli and atypical bacteria (D. tsuruhatensis and Achromobacter xylosoxidans), were evaluated. All species were good biofilm producers (Log 5.84–7.25 CFU cm−2 at 192 h) in artificial urine. The ability of atypical species to form a biofilm appeared to be hampered by the presence of E. coli. Additionally, when E. coli was added to a pre-formed biofilm of the atypical species, it seemed to take advantage of the first colonizers to accelerate adhesion, even when added at lower concentrations. The results suggest a greater ability of E. coli to form biofilms in conditions mimicking the CAUTIs, whatever the pre-existing microbiota and the inoculum concentration. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014 2014-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/31858 |
url |
http://hdl.handle.net/1822/31858 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Azevedo, A. S.; Almeida, Carina; Melo, L. F.; Azevedo, N. F., Interaction between atypical microorganisms and E. coli in catheter-associated urinary tract biofilms. Biofouling: The Journal of Bioadhesion and Biofilm Research, 30(8), 893-902, 2014 0892-7014 1029-2454 10.1080/08927014.2014.944173 25184430 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Taylor and Francis |
publisher.none.fl_str_mv |
Taylor and Francis |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799133066258546688 |