Glutamate regulates the viability of retinal cells in culture

Detalhes bibliográficos
Autor(a) principal: Rego, A. Cristina
Data de Publicação: 2001
Outros Autores: Santos, Maria Sancha, Areias, Filipe, Proença, Teresa, Oliveira, Catarina R.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10316/4830
https://doi.org/10.1016/S0042-6989(00)00309-6
Resumo: In this study, we show that glutamate regulates the viability of cultured retinal cells upon transient glucose deprivation. At low concentrations (10-100 [mu]M) glutamate decreased MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] reduction to about 50% of control and decreased intracellular ATP levels (about 4-fold) after transient glucose removal. Under these conditions, the decrease in MTT reduction was associated with the activation of NMDA (N-methyl--aspartate) receptors. Upon exposure to high (10 mM) glutamate and transient glucose deprivation, the intracellular levels of glutamate increased. High glutamate significantly counteracted the decrease in MTT reduction and ATP production observed in the presence of low glutamate concentrations. AOAA (aminooxyacetic acid), a non-specific inhibitor of mitochondrial transaminases, enhanced the intracellular glutamate levels, but did not largely affect glutamate-mediated changes in MTT reduction or ATP production. Furthermore, the intracellular levels of pyruvate were not significantly altered, suggesting that changes in ATP production were not due to an increase in glycolysis. Thus, the recovery from glucose deprivation seems to be facilitated in retinal neuronal cells that had been exposed to high glutamate, in comparison with low glutamate, suggesting a role for high glutamate and glucose in maintaining retinal cell function following conditions of glucose scarcity.
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spelling Glutamate regulates the viability of retinal cells in cultureExcitotoxicityGlutamateMitochondriaNMDA receptorsRetinal cellsIn this study, we show that glutamate regulates the viability of cultured retinal cells upon transient glucose deprivation. At low concentrations (10-100 [mu]M) glutamate decreased MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] reduction to about 50% of control and decreased intracellular ATP levels (about 4-fold) after transient glucose removal. Under these conditions, the decrease in MTT reduction was associated with the activation of NMDA (N-methyl--aspartate) receptors. Upon exposure to high (10 mM) glutamate and transient glucose deprivation, the intracellular levels of glutamate increased. High glutamate significantly counteracted the decrease in MTT reduction and ATP production observed in the presence of low glutamate concentrations. AOAA (aminooxyacetic acid), a non-specific inhibitor of mitochondrial transaminases, enhanced the intracellular glutamate levels, but did not largely affect glutamate-mediated changes in MTT reduction or ATP production. Furthermore, the intracellular levels of pyruvate were not significantly altered, suggesting that changes in ATP production were not due to an increase in glycolysis. Thus, the recovery from glucose deprivation seems to be facilitated in retinal neuronal cells that had been exposed to high glutamate, in comparison with low glutamate, suggesting a role for high glutamate and glucose in maintaining retinal cell function following conditions of glucose scarcity.http://www.sciencedirect.com/science/article/B6T0W-42HFNK7-F/1/f8f05fb7ba14343adbb0bc8c889b9c222001info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleaplication/PDFhttp://hdl.handle.net/10316/4830http://hdl.handle.net/10316/4830https://doi.org/10.1016/S0042-6989(00)00309-6engVision Research. 41:7 (2001) 841-851Rego, A. CristinaSantos, Maria SanchaAreias, FilipeProença, TeresaOliveira, Catarina R.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2020-11-06T16:59:35Zoai:estudogeral.uc.pt:10316/4830Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:43:28.834038Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Glutamate regulates the viability of retinal cells in culture
title Glutamate regulates the viability of retinal cells in culture
spellingShingle Glutamate regulates the viability of retinal cells in culture
Rego, A. Cristina
Excitotoxicity
Glutamate
Mitochondria
NMDA receptors
Retinal cells
title_short Glutamate regulates the viability of retinal cells in culture
title_full Glutamate regulates the viability of retinal cells in culture
title_fullStr Glutamate regulates the viability of retinal cells in culture
title_full_unstemmed Glutamate regulates the viability of retinal cells in culture
title_sort Glutamate regulates the viability of retinal cells in culture
author Rego, A. Cristina
author_facet Rego, A. Cristina
Santos, Maria Sancha
Areias, Filipe
Proença, Teresa
Oliveira, Catarina R.
author_role author
author2 Santos, Maria Sancha
Areias, Filipe
Proença, Teresa
Oliveira, Catarina R.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Rego, A. Cristina
Santos, Maria Sancha
Areias, Filipe
Proença, Teresa
Oliveira, Catarina R.
dc.subject.por.fl_str_mv Excitotoxicity
Glutamate
Mitochondria
NMDA receptors
Retinal cells
topic Excitotoxicity
Glutamate
Mitochondria
NMDA receptors
Retinal cells
description In this study, we show that glutamate regulates the viability of cultured retinal cells upon transient glucose deprivation. At low concentrations (10-100 [mu]M) glutamate decreased MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] reduction to about 50% of control and decreased intracellular ATP levels (about 4-fold) after transient glucose removal. Under these conditions, the decrease in MTT reduction was associated with the activation of NMDA (N-methyl--aspartate) receptors. Upon exposure to high (10 mM) glutamate and transient glucose deprivation, the intracellular levels of glutamate increased. High glutamate significantly counteracted the decrease in MTT reduction and ATP production observed in the presence of low glutamate concentrations. AOAA (aminooxyacetic acid), a non-specific inhibitor of mitochondrial transaminases, enhanced the intracellular glutamate levels, but did not largely affect glutamate-mediated changes in MTT reduction or ATP production. Furthermore, the intracellular levels of pyruvate were not significantly altered, suggesting that changes in ATP production were not due to an increase in glycolysis. Thus, the recovery from glucose deprivation seems to be facilitated in retinal neuronal cells that had been exposed to high glutamate, in comparison with low glutamate, suggesting a role for high glutamate and glucose in maintaining retinal cell function following conditions of glucose scarcity.
publishDate 2001
dc.date.none.fl_str_mv 2001
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/10316/4830
http://hdl.handle.net/10316/4830
https://doi.org/10.1016/S0042-6989(00)00309-6
url http://hdl.handle.net/10316/4830
https://doi.org/10.1016/S0042-6989(00)00309-6
dc.language.iso.fl_str_mv eng
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dc.relation.none.fl_str_mv Vision Research. 41:7 (2001) 841-851
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