Synergistic and antibiofilm properties of ocellatin peptides against multidrug-resistant Pseudomonas aeruginosa

Detalhes bibliográficos
Autor(a) principal: Bessa, Lucinda J
Data de Publicação: 2018
Outros Autores: Eaton, Peter, Dematei, Anderson, Plácido, Alexandra, Vale, Nuno, Gomes, Paula, Delerue-Matos, Cristina, Leite, José Roberto Sá, Gameiro, Paula
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.22/14762
Resumo: Aim:To test ocellatin peptides (ocellatins-PT2-PT6) for antibacterial and antibiofilm activities and synergy with antibiotics against Pseudomonas aeruginosa. Materials & methods: Normal- and checkerboard-broth microdilution methods were used. Biofilm studies included microtiter plate-based assays and microscopic analysis by confocal laser scanning microscopy and atomic force microscopy. Results: Ocellatins were more active against multidrug-resistant isolates of P. aeruginosa than against susceptible strains. Ocellatin-PT3 showed synergy with ciprofloxacin and ceftazidime against multidrug-resistant isolates and was capable of preventing the proliferation of 48-h mature biofilms at concentrations ranging from 4 to 8× the MIC. Treated biofilms had low viability and were slightly more disaggregated. Conclusion: Ocellatin-PT3 may be promising as a template for the development of novel antimicrobial peptides against P. aeruginosa.
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spelling Synergistic and antibiofilm properties of ocellatin peptides against multidrug-resistant Pseudomonas aeruginosaAMPsAntibiofilm ActivityAntimicrobial peptidesMultidrug-resistant Pseudomonas aeruginosaOcellatin peptidesOcellatin-PT3SynergyAim:To test ocellatin peptides (ocellatins-PT2-PT6) for antibacterial and antibiofilm activities and synergy with antibiotics against Pseudomonas aeruginosa. Materials & methods: Normal- and checkerboard-broth microdilution methods were used. Biofilm studies included microtiter plate-based assays and microscopic analysis by confocal laser scanning microscopy and atomic force microscopy. Results: Ocellatins were more active against multidrug-resistant isolates of P. aeruginosa than against susceptible strains. Ocellatin-PT3 showed synergy with ciprofloxacin and ceftazidime against multidrug-resistant isolates and was capable of preventing the proliferation of 48-h mature biofilms at concentrations ranging from 4 to 8× the MIC. Treated biofilms had low viability and were slightly more disaggregated. Conclusion: Ocellatin-PT3 may be promising as a template for the development of novel antimicrobial peptides against P. aeruginosa.This work received financial support from the European Union (FEDER funds through COMPETE) and National Funds (FCT, Fundação para a Ciência e Tecnologia), under the Partnership Agreement PT2020 through project UID/QUI/50006/2013-POCI/01/0145/FEDER/007265 (LAQV/REQUIMTE) and from Programa Operacional Regional do Norte (ON.2 – O Novo Norte),under the Quadro de Referencia Estrat ˆ egico Nacional (QREN) and funded by Fundo Europeu de Desenvolvimento Regional ´ (Feder) through projects: NORTE-01-0145-FEDER-000024, NORTE-01-0145-FEDER-000011 and NORTE-07-0161-FEDER-000111.A Placido is grateful to FCT for financial support through the grant SFRH ´ /BD/97995/2013, POPH-QREN-Tipologia 4.1-Formação Avançada and Fundo Social Europeu and Ministerio da Ciência, Tecnologia e Ensino Superior. The authors have no other relevant ˆ affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.Future MedicineRepositório Científico do Instituto Politécnico do PortoBessa, Lucinda JEaton, PeterDematei, AndersonPlácido, AlexandraVale, NunoGomes, PaulaDelerue-Matos, CristinaLeite, José Roberto SáGameiro, Paula2019-10-29T15:30:36Z20182018-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.22/14762eng1746-091310.2217/fmb-2017-0175info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-13T12:57:49Zoai:recipp.ipp.pt:10400.22/14762Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:34:15.670511Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Synergistic and antibiofilm properties of ocellatin peptides against multidrug-resistant Pseudomonas aeruginosa
title Synergistic and antibiofilm properties of ocellatin peptides against multidrug-resistant Pseudomonas aeruginosa
spellingShingle Synergistic and antibiofilm properties of ocellatin peptides against multidrug-resistant Pseudomonas aeruginosa
Bessa, Lucinda J
AMPs
Antibiofilm Activity
Antimicrobial peptides
Multidrug-resistant Pseudomonas aeruginosa
Ocellatin peptides
Ocellatin-PT3
Synergy
title_short Synergistic and antibiofilm properties of ocellatin peptides against multidrug-resistant Pseudomonas aeruginosa
title_full Synergistic and antibiofilm properties of ocellatin peptides against multidrug-resistant Pseudomonas aeruginosa
title_fullStr Synergistic and antibiofilm properties of ocellatin peptides against multidrug-resistant Pseudomonas aeruginosa
title_full_unstemmed Synergistic and antibiofilm properties of ocellatin peptides against multidrug-resistant Pseudomonas aeruginosa
title_sort Synergistic and antibiofilm properties of ocellatin peptides against multidrug-resistant Pseudomonas aeruginosa
author Bessa, Lucinda J
author_facet Bessa, Lucinda J
Eaton, Peter
Dematei, Anderson
Plácido, Alexandra
Vale, Nuno
Gomes, Paula
Delerue-Matos, Cristina
Leite, José Roberto Sá
Gameiro, Paula
author_role author
author2 Eaton, Peter
Dematei, Anderson
Plácido, Alexandra
Vale, Nuno
Gomes, Paula
Delerue-Matos, Cristina
Leite, José Roberto Sá
Gameiro, Paula
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório Científico do Instituto Politécnico do Porto
dc.contributor.author.fl_str_mv Bessa, Lucinda J
Eaton, Peter
Dematei, Anderson
Plácido, Alexandra
Vale, Nuno
Gomes, Paula
Delerue-Matos, Cristina
Leite, José Roberto Sá
Gameiro, Paula
dc.subject.por.fl_str_mv AMPs
Antibiofilm Activity
Antimicrobial peptides
Multidrug-resistant Pseudomonas aeruginosa
Ocellatin peptides
Ocellatin-PT3
Synergy
topic AMPs
Antibiofilm Activity
Antimicrobial peptides
Multidrug-resistant Pseudomonas aeruginosa
Ocellatin peptides
Ocellatin-PT3
Synergy
description Aim:To test ocellatin peptides (ocellatins-PT2-PT6) for antibacterial and antibiofilm activities and synergy with antibiotics against Pseudomonas aeruginosa. Materials & methods: Normal- and checkerboard-broth microdilution methods were used. Biofilm studies included microtiter plate-based assays and microscopic analysis by confocal laser scanning microscopy and atomic force microscopy. Results: Ocellatins were more active against multidrug-resistant isolates of P. aeruginosa than against susceptible strains. Ocellatin-PT3 showed synergy with ciprofloxacin and ceftazidime against multidrug-resistant isolates and was capable of preventing the proliferation of 48-h mature biofilms at concentrations ranging from 4 to 8× the MIC. Treated biofilms had low viability and were slightly more disaggregated. Conclusion: Ocellatin-PT3 may be promising as a template for the development of novel antimicrobial peptides against P. aeruginosa.
publishDate 2018
dc.date.none.fl_str_mv 2018
2018-01-01T00:00:00Z
2019-10-29T15:30:36Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.22/14762
url http://hdl.handle.net/10400.22/14762
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1746-0913
10.2217/fmb-2017-0175
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Future Medicine
publisher.none.fl_str_mv Future Medicine
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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