Proteomic Analysis of a Syntrophic Coculture of Syntrophobacter fumaroxidans MPOBT and Geobacter sulfurreducens PCAT

Detalhes bibliográficos
Autor(a) principal: Mollaei, Monir
Data de Publicação: 2021
Outros Autores: Suarez-Diez, Maria, Sedano-Nunez, Vicente T., Boeren, Sjef, Stams, Alfons Johannes Maria, Plugge, Caroline M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/75259
Resumo: The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fmicb.2021.708911/full#supplementary-material
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spelling Proteomic Analysis of a Syntrophic Coculture of Syntrophobacter fumaroxidans MPOBT and Geobacter sulfurreducens PCATSyntrophobacter fumaroxidansGeobacter sulfurreducenscocultureinterspecies electron transferpropionateproteomicsCiências Naturais::Ciências BiológicasScience & TechnologyThe Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fmicb.2021.708911/full#supplementary-materialWe established a syntrophic coculture of Syntrophobacter fumaroxidans MPOB<sup>T</sup> (SF) and Geobacter sulfurreducens PCA<sup>T</sup> (GS) growing on propionate and Fe(III). Neither of the bacteria was capable of growth on propionate and Fe(III) in pure culture. Propionate degradation by SF provides acetate, hydrogen, and/or formate that can be used as electron donors by GS with Fe(III) citrate as electron acceptor. Proteomic analyses of the SF-GS coculture revealed propionate conversion via the methylmalonyl-CoA (MMC) pathway by SF. The possibility of interspecies electron transfer (IET) via direct (DIET) and/or hydrogen/formate transfer (HFIT) was investigated by comparing the differential abundance of associated proteins in SF-GS coculture against (i) SF coculture with Methanospirillum hungatei (SF-MH), which relies on HFIT, (ii) GS pure culture growing on acetate, formate, hydrogen as propionate products, and Fe(III). We noted some evidence for DIET in the SF-GS coculture, i.e., GS in the coculture showed significantly lower abundance of uptake hydrogenase (43-fold) and formate dehydrogenase (45-fold) and significantly higher abundance of proteins related to acetate metabolism (i.e., GltA; 62-fold) compared to GS pure culture. Moreover, SF in the SF-GS coculture showed significantly lower abundance of IET-related formate dehydrogenases, Fdh3 (51-fold) and Fdh5 (29-fold), and the rate of propionate conversion in SF-GS was 8-fold lower than in the SF-MH coculture. In contrast, compared to GS pure culture, we found lower abundance of pilus-associated cytochrome OmcS (2-fold) and piliA (5-fold) in the SF-GS coculture that is suggested to be necessary for DIET. Furthermore, neither visible aggregates formed in the SF-GS coculture, nor the pili-E of SF (suggested as e-pili) were detected. These findings suggest that the IET mechanism is complex in the SF-GS coculture and can be mediated by several mechanisms rather than one discrete pathway. Our study can be further useful in understanding syntrophic propionate degradation in bioelectrochemical and anaerobic digestion systems.This work was performed in the TTIW-cooperation framework of Wetsus, European Centre of Excellence for Sustainable Water Technology (www.wetsus.nl). Wetsus was funded by the Dutch Ministry of Economic Affairs, the European Union Regional Development Fund, the Province of Fryslân, the City of Leeuwarden, and the EZ/Kompas program of the “Samenwerkingsverband Noord-Nederland”. Research of AJMS was financed by an advanced grant of the European Research Council under the European Union’s Seventh Framework Programme (FP/2007e2013)/ERC Grant Agreement (project 323009) and a Gravitation grant (project 024.002.002) of the Netherlands Ministry of Education, Culture and Science.info:eu-repo/semantics/publishedVersionFrontiers Media S.A.Universidade do MinhoMollaei, MonirSuarez-Diez, MariaSedano-Nunez, Vicente T.Boeren, SjefStams, Alfons Johannes MariaPlugge, Caroline M.2021-11-302021-11-30T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/75259engMollaei, Monir; Suarez-Diez, Maria; Sedano-Nunez, Vicente T.; Boeren, Sjef; Stams, A. J. M.; Plugge, Caroline M., Proteomic Analysis of a Syntrophic Coculture of Syntrophobacter fumaroxidans MPOBT and Geobacter sulfurreducens PCAT. Frontiers in Microbiology, 12(708911), 20211664302X10.3389/fmicb.2021.708911https://www.frontiersin.org/articles/10.3389/fmicb.2021.708911/fullinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:49:03Zoai:repositorium.sdum.uminho.pt:1822/75259Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:47:26.715972Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Proteomic Analysis of a Syntrophic Coculture of Syntrophobacter fumaroxidans MPOBT and Geobacter sulfurreducens PCAT
title Proteomic Analysis of a Syntrophic Coculture of Syntrophobacter fumaroxidans MPOBT and Geobacter sulfurreducens PCAT
spellingShingle Proteomic Analysis of a Syntrophic Coculture of Syntrophobacter fumaroxidans MPOBT and Geobacter sulfurreducens PCAT
Mollaei, Monir
Syntrophobacter fumaroxidans
Geobacter sulfurreducens
coculture
interspecies electron transfer
propionate
proteomics
Ciências Naturais::Ciências Biológicas
Science & Technology
title_short Proteomic Analysis of a Syntrophic Coculture of Syntrophobacter fumaroxidans MPOBT and Geobacter sulfurreducens PCAT
title_full Proteomic Analysis of a Syntrophic Coculture of Syntrophobacter fumaroxidans MPOBT and Geobacter sulfurreducens PCAT
title_fullStr Proteomic Analysis of a Syntrophic Coculture of Syntrophobacter fumaroxidans MPOBT and Geobacter sulfurreducens PCAT
title_full_unstemmed Proteomic Analysis of a Syntrophic Coculture of Syntrophobacter fumaroxidans MPOBT and Geobacter sulfurreducens PCAT
title_sort Proteomic Analysis of a Syntrophic Coculture of Syntrophobacter fumaroxidans MPOBT and Geobacter sulfurreducens PCAT
author Mollaei, Monir
author_facet Mollaei, Monir
Suarez-Diez, Maria
Sedano-Nunez, Vicente T.
Boeren, Sjef
Stams, Alfons Johannes Maria
Plugge, Caroline M.
author_role author
author2 Suarez-Diez, Maria
Sedano-Nunez, Vicente T.
Boeren, Sjef
Stams, Alfons Johannes Maria
Plugge, Caroline M.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Mollaei, Monir
Suarez-Diez, Maria
Sedano-Nunez, Vicente T.
Boeren, Sjef
Stams, Alfons Johannes Maria
Plugge, Caroline M.
dc.subject.por.fl_str_mv Syntrophobacter fumaroxidans
Geobacter sulfurreducens
coculture
interspecies electron transfer
propionate
proteomics
Ciências Naturais::Ciências Biológicas
Science & Technology
topic Syntrophobacter fumaroxidans
Geobacter sulfurreducens
coculture
interspecies electron transfer
propionate
proteomics
Ciências Naturais::Ciências Biológicas
Science & Technology
description The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fmicb.2021.708911/full#supplementary-material
publishDate 2021
dc.date.none.fl_str_mv 2021-11-30
2021-11-30T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/75259
url http://hdl.handle.net/1822/75259
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Mollaei, Monir; Suarez-Diez, Maria; Sedano-Nunez, Vicente T.; Boeren, Sjef; Stams, A. J. M.; Plugge, Caroline M., Proteomic Analysis of a Syntrophic Coculture of Syntrophobacter fumaroxidans MPOBT and Geobacter sulfurreducens PCAT. Frontiers in Microbiology, 12(708911), 2021
1664302X
10.3389/fmicb.2021.708911
https://www.frontiersin.org/articles/10.3389/fmicb.2021.708911/full
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media S.A.
publisher.none.fl_str_mv Frontiers Media S.A.
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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