Selection and subsequent physiological characterization of industrial Saccharomyces cerevisiae strains during continuous growth at sub- and- supra optimal temperatures

Detalhes bibliográficos
Autor(a) principal: Lip, Ka Ying Florence
Data de Publicação: 2020
Outros Autores: García-Ríos, Estéfani, Costa, Carlos E., José Manuel Guillamón, Domingues, Lucília, Teixeira, J. A., van Gulik, Walter M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/65538
Resumo: Supplementary material related to this article can be found, in the online version, at doi:https://doi.org/10.1016/j.btre.2020.e00462.
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spelling Selection and subsequent physiological characterization of industrial Saccharomyces cerevisiae strains during continuous growth at sub- and- supra optimal temperaturesChemostatEnergetic efficiencyTemperature toleranceSaccharomycesSBRSupplementary material related to this article can be found, in the online version, at doi:https://doi.org/10.1016/j.btre.2020.e00462.A phenotypic screening of 12 industrial yeast strains and the well-studied laboratory strain CEN.PK113-7D at cultivation temperatures between 12°C and 40°C revealed significant differences in maximum growth rates and temperature tolerance. From those 12, two strains, one performing best at 12°C and the other at 40°C, plus the laboratory strain, were selected for further physiological characterization in well-controlled bioreactors. The strains were grown in anaerobic chemostats, at a fixed specific growth rate of 0.03h1 and sequential batch cultures at 12°C, 30°C, and 39°C. We observed significant differences in biomass and ethanol yields on glucose, biomass protein and storage carbohydrate contents, and biomass yields on ATP between strains and cultivation temperatures. Increased temperature tolerance coincided with higher energetic efficiency of cell growth, indicating that temperature intolerance is a result of energy wasting processes, such as increased turnover of cellular components (e.g. proteins) due to temperature induced damage.We would like to thank Judith Cohen and Kristen H. David for technical assistance with the chemostat fermentations and José Ma Heras (Lallemand Ibéria, SA) for kindly providing the industrial strains. This research was carried out within the ERA-IB project “YeastTempTation” (ERA-IB-2-6/0001/2014) and partially supported by the Portuguese Foundation for Science and Technology (FCT) through strategic funding UID/BIO/04469/2020 and BioTecNorte (NORTE-01-0145-FEDER-000004).info:eu-repo/semantics/publishedVersionElsevier BVUniversidade do MinhoLip, Ka Ying FlorenceGarcía-Ríos, EstéfaniCosta, Carlos E.José Manuel GuillamónDomingues, LucíliaTeixeira, J. A.van Gulik, Walter M.2020-062020-06-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/65538engLip, Ka Ying Florence; García-Ríos, Estéfani; Costa, Carlos E.; José Manuel Guillamón; Domingues, Lucília; Teixeira, José A.; van Gulik, Walter M., Selection and subsequent physiological characterization of industrial Saccharomyces cerevisiae strains during continuous growth at sub- and- supra optimal temperatures. Biotechnology Reports, 26(e00462), 20202215-017X10.1016/j.btre.2020.e00462https://www.journals.elsevier.com/biotechnology-reportsinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:28:59Zoai:repositorium.sdum.uminho.pt:1822/65538Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:23:53.760408Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Selection and subsequent physiological characterization of industrial Saccharomyces cerevisiae strains during continuous growth at sub- and- supra optimal temperatures
title Selection and subsequent physiological characterization of industrial Saccharomyces cerevisiae strains during continuous growth at sub- and- supra optimal temperatures
spellingShingle Selection and subsequent physiological characterization of industrial Saccharomyces cerevisiae strains during continuous growth at sub- and- supra optimal temperatures
Lip, Ka Ying Florence
Chemostat
Energetic efficiency
Temperature tolerance
Saccharomyces
SBR
title_short Selection and subsequent physiological characterization of industrial Saccharomyces cerevisiae strains during continuous growth at sub- and- supra optimal temperatures
title_full Selection and subsequent physiological characterization of industrial Saccharomyces cerevisiae strains during continuous growth at sub- and- supra optimal temperatures
title_fullStr Selection and subsequent physiological characterization of industrial Saccharomyces cerevisiae strains during continuous growth at sub- and- supra optimal temperatures
title_full_unstemmed Selection and subsequent physiological characterization of industrial Saccharomyces cerevisiae strains during continuous growth at sub- and- supra optimal temperatures
title_sort Selection and subsequent physiological characterization of industrial Saccharomyces cerevisiae strains during continuous growth at sub- and- supra optimal temperatures
author Lip, Ka Ying Florence
author_facet Lip, Ka Ying Florence
García-Ríos, Estéfani
Costa, Carlos E.
José Manuel Guillamón
Domingues, Lucília
Teixeira, J. A.
van Gulik, Walter M.
author_role author
author2 García-Ríos, Estéfani
Costa, Carlos E.
José Manuel Guillamón
Domingues, Lucília
Teixeira, J. A.
van Gulik, Walter M.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Lip, Ka Ying Florence
García-Ríos, Estéfani
Costa, Carlos E.
José Manuel Guillamón
Domingues, Lucília
Teixeira, J. A.
van Gulik, Walter M.
dc.subject.por.fl_str_mv Chemostat
Energetic efficiency
Temperature tolerance
Saccharomyces
SBR
topic Chemostat
Energetic efficiency
Temperature tolerance
Saccharomyces
SBR
description Supplementary material related to this article can be found, in the online version, at doi:https://doi.org/10.1016/j.btre.2020.e00462.
publishDate 2020
dc.date.none.fl_str_mv 2020-06
2020-06-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/65538
url http://hdl.handle.net/1822/65538
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Lip, Ka Ying Florence; García-Ríos, Estéfani; Costa, Carlos E.; José Manuel Guillamón; Domingues, Lucília; Teixeira, José A.; van Gulik, Walter M., Selection and subsequent physiological characterization of industrial Saccharomyces cerevisiae strains during continuous growth at sub- and- supra optimal temperatures. Biotechnology Reports, 26(e00462), 2020
2215-017X
10.1016/j.btre.2020.e00462
https://www.journals.elsevier.com/biotechnology-reports
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier BV
publisher.none.fl_str_mv Elsevier BV
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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