Advances in chemical and biological methods to identify microorganisms—from past to present

• Autor(a) principal: Franco-Duarte, R
• Data de Publicação: 2019
• Outros Autores: Cernáková, L, Kadam, S, Kaushik, K, Salehi, B, Bevilacqua, A, Corbo, M, Antolak, H, Dybka-Stepien, K, Leszczewicz, M, Tintino, S, Souza, V, Sharifi-Rad, J, Coutinho, H, Martins, N, Rodrigues, C
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: https://hdl.handle.net/10216/137943
• topic: Chromogenic media; DNA fingerprinting; Identification methods; MALDI-TOF; Microscopy techniques; PCR; RAPD PCR; Real-time PCR; WGS

Fast detection and identification of microorganisms is a challenging and significant feature from industry to medicine. Standard approaches are known to be very time-consuming and labor-intensive (e.g., culture media and biochemical tests). Conversely, screening techniques demand a quick and low-cost grouping of bacterial/fungal isolates and current analysis call for broad reports of microorganisms, involving the application of molecular techniques (e.g., 16S ribosomal RNA gene sequencing based on polymerase chain reaction). The goal of this review is to present the past and the present methods of detection and identification of microorganisms, and to discuss their advantages and their limitations.