Cork extracts reduce UV-mediated DNA fragmentation and cell death
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2015 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/1822/38334 |
Resumo: | Accepted Manuscript |
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Cork extracts reduce UV-mediated DNA fragmentation and cell deathAntioxidant activityPolyphenolsUV radiationCiências Médicas::Biotecnologia MédicaScience & TechnologyAccepted ManuscriptUV radiation is known to induce the premature aging of human skin and to contribute to the occurrence of different skin cancers. High doses of UVA (able to penetrate through the epidermis into the dermis) and/or UVB radiation (only affecting the epidermis) leads to cellular oxidative damage compromising the recovery of the normal functions of the cells. This cellular damage is mainly driven by the generation of reactive oxygen species (ROS) that alter the redox status of the intracellular milieu, affecting the cellular metabolic activity, leading to DNA damage, apoptosis and, consequently, to a drastic decrease in the number of live cells, compromising the function of the skin. A series of polyphenolic fractions were extracted from the outer bark (cork) of Quercus suber L., and tested for their capacity to reduce the cellular damage promoted by the ROS produced during UV exposure. This was evaluated after exposing L929 fibroblasts to UV radiation in the presence and absence of the cork extracts. In all the cases the extracts at the concentration of 75μg/ml demonstrated the capacity to preserve cell metabolic activity and their typical morphology, as well as, to avoid DNA fragmentation after exposure to UV radiation. We were also able to correlate these findings with the intracellular reduction of ROS species and the presence of higher proportions of castalagin and vescalagin in the extracts. Our data proves that cork is a relevant source of antioxidant compounds able to act in the cellular environment, protecting cells against oxidation, reducing the number of ROS species and limiting the negative impact of UV radiation. These extracts can be further exploited in the preparation of anti-UV formulations for skin protection.The authors are grateful to Amorim Cork Composites for providing the cork powder raw material and for the financing by the COMPETE/QREN/EU funding program through the project with acronym BioActiveCork (QREN FCOMP-01-0202-FEDER-005455). Ivo M. Aroso and Mariana T. Cerqueira acknowledge the financial support of FCT through the grants SFRH/BD/42273/2007 and SFRH/BPD/96611/2013, respectively. Funding was also granted from the European Union's Seventh Framework Programme (FP7/2007-2013) under grant agreement no. REGPOT-CT2012-316331-POLARIS and from project "Novel smart and biomimetic materials for innovative regenerative medicine approaches (Ref.: RL1-ABMR-NORTE-01-0124-FEDER-000016)" co-financed by North Portugal Regional Operational Programme (ON.2 and "O Novo Norte"), under the National Strategic Reference Framework (NSRF), through the European Regional Development Fund (ERDF) as appropriate.Royal Society of ChemistryUniversidade do MinhoAraújo, A. R.Pereira, D. M.Aroso, Ivo Manuel AscensãoSantos, T. C.Batista, M. T.Cerqueira, M. T.Marques, A. P.Reis, R. L.Pires, R. A.2015-112015-11-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/38334engAraújo A. R., Pereira D. M., Santos T., Batista M. T., Cerqueira M. T., Marques A. P., Reis R. L., Pires R. A. Cork extracts reduce UV-mediated DNA fragmentation and cell death, RSC Advances , Issue 5, pp. 96151 - 96157, doi:0.1039/C5RA15712A, 20152046-206910.1039/c5ra15712ahttp://pubs.rsc.org/en/content/articlelanding/2015/ra/c5ra15712a/unauth#!divAbstractinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:33:39Zoai:repositorium.sdum.uminho.pt:1822/38334Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:29:12.322492Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Cork extracts reduce UV-mediated DNA fragmentation and cell death |
| title |
Cork extracts reduce UV-mediated DNA fragmentation and cell death |
| spellingShingle |
Cork extracts reduce UV-mediated DNA fragmentation and cell death Araújo, A. R. Antioxidant activity Polyphenols UV radiation Ciências Médicas::Biotecnologia Médica Science & Technology |
| title_short |
Cork extracts reduce UV-mediated DNA fragmentation and cell death |
| title_full |
Cork extracts reduce UV-mediated DNA fragmentation and cell death |
| title_fullStr |
Cork extracts reduce UV-mediated DNA fragmentation and cell death |
| title_full_unstemmed |
Cork extracts reduce UV-mediated DNA fragmentation and cell death |
| title_sort |
Cork extracts reduce UV-mediated DNA fragmentation and cell death |
| author |
Araújo, A. R. |
| author_facet |
Araújo, A. R. Pereira, D. M. Aroso, Ivo Manuel Ascensão Santos, T. C. Batista, M. T. Cerqueira, M. T. Marques, A. P. Reis, R. L. Pires, R. A. |
| author_role |
author |
| author2 |
Pereira, D. M. Aroso, Ivo Manuel Ascensão Santos, T. C. Batista, M. T. Cerqueira, M. T. Marques, A. P. Reis, R. L. Pires, R. A. |
| author2_role |
author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Universidade do Minho |
| dc.contributor.author.fl_str_mv |
Araújo, A. R. Pereira, D. M. Aroso, Ivo Manuel Ascensão Santos, T. C. Batista, M. T. Cerqueira, M. T. Marques, A. P. Reis, R. L. Pires, R. A. |
| dc.subject.por.fl_str_mv |
Antioxidant activity Polyphenols UV radiation Ciências Médicas::Biotecnologia Médica Science & Technology |
| topic |
Antioxidant activity Polyphenols UV radiation Ciências Médicas::Biotecnologia Médica Science & Technology |
| description |
Accepted Manuscript |
| publishDate |
2015 |
| dc.date.none.fl_str_mv |
2015-11 2015-11-01T00:00:00Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/38334 |
| url |
http://hdl.handle.net/1822/38334 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Araújo A. R., Pereira D. M., Santos T., Batista M. T., Cerqueira M. T., Marques A. P., Reis R. L., Pires R. A. Cork extracts reduce UV-mediated DNA fragmentation and cell death, RSC Advances , Issue 5, pp. 96151 - 96157, doi:0.1039/C5RA15712A, 2015 2046-2069 10.1039/c5ra15712a http://pubs.rsc.org/en/content/articlelanding/2015/ra/c5ra15712a/unauth#!divAbstract |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
| dc.publisher.none.fl_str_mv |
Royal Society of Chemistry |
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Royal Society of Chemistry |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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