A biomimetic photonic crystal sensor for label-free detection of urinary venous thromboembolism biomarker
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.22/18590 |
Resumo: | This work reports a novel label-free sensor combining photonic crystals and molecularly-imprinted polymer materials for targeting a protein in human samples within levels of clinical interest. This concept was applied to detect fibrinopeptide B (FPB) in urine, a biomarker of venous thromboembolism, which is a disease of great concern and demands innovative point-of-care devices for improved diagnosis. The molecularly-imprinted photonic polymer (MIPP) was obtained by tailoring an imprinted polymer on highly ordered silica nanoparticles assembled by vertical deposition. Owing to the hierarchical structure, the resulting MIPP exhibited optical properties that changed upon rebinding of the target analyte, FPB. Thus, the changes in reflectance intensity enabled a rapid and sensitive detection of FPB in human urine. The observed response was linear between 0.2 ng mL−1 and 22 ng mL-1 and showed a limit of detection of 0.13 ng mL-1. These features of the sensing material allow the assessment of urinary FPB at relevant clinical levels. In addition, the sensor was selective for FPB compared to the standard biomarker of venous thromboembolism, D-Dimer. The stability of the material was evidenced by the reusability study, which demonstrated reversibility of the maximum intensity after three cycles of recognition and regeneration. Moreover, the sensor showed good performance for the spiked FPB detection in control human urine. Overall, the application of such label-free sensor offers high selectivity and stability, as well as easy operation. It may constitute an alternative method for non-invasive and real-time detection of various protein biomarkers in point-of-care. |
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A biomimetic photonic crystal sensor for label-free detection of urinary venous thromboembolism biomarkerPhotonic sensorPhotonic crystalMolecularly-imprinted polymerFibrinopeptide BVenous thromboembolism biomarkerThis work reports a novel label-free sensor combining photonic crystals and molecularly-imprinted polymer materials for targeting a protein in human samples within levels of clinical interest. This concept was applied to detect fibrinopeptide B (FPB) in urine, a biomarker of venous thromboembolism, which is a disease of great concern and demands innovative point-of-care devices for improved diagnosis. The molecularly-imprinted photonic polymer (MIPP) was obtained by tailoring an imprinted polymer on highly ordered silica nanoparticles assembled by vertical deposition. Owing to the hierarchical structure, the resulting MIPP exhibited optical properties that changed upon rebinding of the target analyte, FPB. Thus, the changes in reflectance intensity enabled a rapid and sensitive detection of FPB in human urine. The observed response was linear between 0.2 ng mL−1 and 22 ng mL-1 and showed a limit of detection of 0.13 ng mL-1. These features of the sensing material allow the assessment of urinary FPB at relevant clinical levels. In addition, the sensor was selective for FPB compared to the standard biomarker of venous thromboembolism, D-Dimer. The stability of the material was evidenced by the reusability study, which demonstrated reversibility of the maximum intensity after three cycles of recognition and regeneration. Moreover, the sensor showed good performance for the spiked FPB detection in control human urine. Overall, the application of such label-free sensor offers high selectivity and stability, as well as easy operation. It may constitute an alternative method for non-invasive and real-time detection of various protein biomarkers in point-of-care.The authors gratefully acknowledge the financial support from FEDER (european regional development fund), Fundação para a Ciência e Tecnologia (FCT) and INTERREG (previously missing here), through projects STRIP2SENSE (NORTE-01-0145-FEDER-024358-SAICT-POL/24358/2016) and IBEROS (Instituto de Bioingeniería en Red para el Envejecimiento Saludable, INTERREG POCTEP/0245_IBEROS_1_E). Sara Resende thanks FCT for the PhD grant ref. SFRH/BD/139634/2018.ElsevierRepositório Científico do Instituto Politécnico do PortoResende, SaraFrasco, Manuela F.Sales, Maria Goreti Ferreira2021-09-28T08:48:45Z20202020-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.22/18590engResende S, Frasco MF, Sales MGF, A biomimetic photonic crystal sensor for label-free detection of urinary venous thromboembolism biomarker, Sensors and Actuators: B. Chemical (2020), doi:https://doi.org/10.1016/j.snb.2020.12794710.1016/j.snb.2020.127947info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-13T13:09:58Zoai:recipp.ipp.pt:10400.22/18590Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:37:55.490316Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
A biomimetic photonic crystal sensor for label-free detection of urinary venous thromboembolism biomarker |
title |
A biomimetic photonic crystal sensor for label-free detection of urinary venous thromboembolism biomarker |
spellingShingle |
A biomimetic photonic crystal sensor for label-free detection of urinary venous thromboembolism biomarker Resende, Sara Photonic sensor Photonic crystal Molecularly-imprinted polymer Fibrinopeptide B Venous thromboembolism biomarker |
title_short |
A biomimetic photonic crystal sensor for label-free detection of urinary venous thromboembolism biomarker |
title_full |
A biomimetic photonic crystal sensor for label-free detection of urinary venous thromboembolism biomarker |
title_fullStr |
A biomimetic photonic crystal sensor for label-free detection of urinary venous thromboembolism biomarker |
title_full_unstemmed |
A biomimetic photonic crystal sensor for label-free detection of urinary venous thromboembolism biomarker |
title_sort |
A biomimetic photonic crystal sensor for label-free detection of urinary venous thromboembolism biomarker |
author |
Resende, Sara |
author_facet |
Resende, Sara Frasco, Manuela F. Sales, Maria Goreti Ferreira |
author_role |
author |
author2 |
Frasco, Manuela F. Sales, Maria Goreti Ferreira |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Politécnico do Porto |
dc.contributor.author.fl_str_mv |
Resende, Sara Frasco, Manuela F. Sales, Maria Goreti Ferreira |
dc.subject.por.fl_str_mv |
Photonic sensor Photonic crystal Molecularly-imprinted polymer Fibrinopeptide B Venous thromboembolism biomarker |
topic |
Photonic sensor Photonic crystal Molecularly-imprinted polymer Fibrinopeptide B Venous thromboembolism biomarker |
description |
This work reports a novel label-free sensor combining photonic crystals and molecularly-imprinted polymer materials for targeting a protein in human samples within levels of clinical interest. This concept was applied to detect fibrinopeptide B (FPB) in urine, a biomarker of venous thromboembolism, which is a disease of great concern and demands innovative point-of-care devices for improved diagnosis. The molecularly-imprinted photonic polymer (MIPP) was obtained by tailoring an imprinted polymer on highly ordered silica nanoparticles assembled by vertical deposition. Owing to the hierarchical structure, the resulting MIPP exhibited optical properties that changed upon rebinding of the target analyte, FPB. Thus, the changes in reflectance intensity enabled a rapid and sensitive detection of FPB in human urine. The observed response was linear between 0.2 ng mL−1 and 22 ng mL-1 and showed a limit of detection of 0.13 ng mL-1. These features of the sensing material allow the assessment of urinary FPB at relevant clinical levels. In addition, the sensor was selective for FPB compared to the standard biomarker of venous thromboembolism, D-Dimer. The stability of the material was evidenced by the reusability study, which demonstrated reversibility of the maximum intensity after three cycles of recognition and regeneration. Moreover, the sensor showed good performance for the spiked FPB detection in control human urine. Overall, the application of such label-free sensor offers high selectivity and stability, as well as easy operation. It may constitute an alternative method for non-invasive and real-time detection of various protein biomarkers in point-of-care. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020 2020-01-01T00:00:00Z 2021-09-28T08:48:45Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.22/18590 |
url |
http://hdl.handle.net/10400.22/18590 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Resende S, Frasco MF, Sales MGF, A biomimetic photonic crystal sensor for label-free detection of urinary venous thromboembolism biomarker, Sensors and Actuators: B. Chemical (2020), doi:https://doi.org/10.1016/j.snb.2020.127947 10.1016/j.snb.2020.127947 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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