Fish Oil Enzymatic Esterification for Acidity Reduction
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.22/17913 |
Resumo: | The reduction of the fish oil acidity is a significant problem in the rendering industry, as the oil’s range of applications and market value strongly depend on this parameter. In particular, the lower the acidity, the larger the oil’s market value. This work aims to study the potential of enzymatic esterification for reducing the fish oil acidity, by converting the free fatty acids into esters. Thus, four commercial lipases were used and a parametric study was performed to identify the best operating conditions, varying the reaction temperature, enzyme/oil mass ratio and alcohol/FFA mass ratio. All experiments were performed in duplicate with a very good reproducibility of results. Results showed that Lipozyme TL 100L contributed to greater acidity reduction (75% from an initial acid value of 10–14 mg KOH/g oil) for esterification at 40 °C, using ethanol 96% v/v, enzyme/oil and alcohol/FFA mass ratios of 0.01 and 3.24 w/w, respectively, reaching 3.13 mg KOH/g oil of final acid value or 1.57% FFA content. The reaction kinetics were also studied and it was found that a second order rate law as a function of the alcohol and oil concentrations is more adequate, with 35.44 kJ/mol of activation energy and 1.94 × 103 L mol− 1 min− 1 of pre-exponential factor. In conclusion, this work shows that the enzymatic esterification to reduce the fish oil acidity is technically feasible, increasing its market value. |
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7160 |
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Fish Oil Enzymatic Esterification for Acidity ReductionAcidity reductionEnzymatic esterificationFish oilFree fatty acidsLipozyme TL 100LThe reduction of the fish oil acidity is a significant problem in the rendering industry, as the oil’s range of applications and market value strongly depend on this parameter. In particular, the lower the acidity, the larger the oil’s market value. This work aims to study the potential of enzymatic esterification for reducing the fish oil acidity, by converting the free fatty acids into esters. Thus, four commercial lipases were used and a parametric study was performed to identify the best operating conditions, varying the reaction temperature, enzyme/oil mass ratio and alcohol/FFA mass ratio. All experiments were performed in duplicate with a very good reproducibility of results. Results showed that Lipozyme TL 100L contributed to greater acidity reduction (75% from an initial acid value of 10–14 mg KOH/g oil) for esterification at 40 °C, using ethanol 96% v/v, enzyme/oil and alcohol/FFA mass ratios of 0.01 and 3.24 w/w, respectively, reaching 3.13 mg KOH/g oil of final acid value or 1.57% FFA content. The reaction kinetics were also studied and it was found that a second order rate law as a function of the alcohol and oil concentrations is more adequate, with 35.44 kJ/mol of activation energy and 1.94 × 103 L mol− 1 min− 1 of pre-exponential factor. In conclusion, this work shows that the enzymatic esterification to reduce the fish oil acidity is technically feasible, increasing its market value.SpringerRepositório Científico do Instituto Politécnico do PortoMata, Teresa M.Correia, DanielaAndrade, SoraiaCasal, SusanaFerreira, Isabel M. P. L. V. O.Matos, ElisabeteMartins, António A.Caetano, Nídia20202100-01-01T00:00:00Z2020-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.22/17913eng10.1007/s12649-018-0357-zmetadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-13T13:09:07Zoai:recipp.ipp.pt:10400.22/17913Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:37:30.537524Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Fish Oil Enzymatic Esterification for Acidity Reduction |
title |
Fish Oil Enzymatic Esterification for Acidity Reduction |
spellingShingle |
Fish Oil Enzymatic Esterification for Acidity Reduction Mata, Teresa M. Acidity reduction Enzymatic esterification Fish oil Free fatty acids Lipozyme TL 100L |
title_short |
Fish Oil Enzymatic Esterification for Acidity Reduction |
title_full |
Fish Oil Enzymatic Esterification for Acidity Reduction |
title_fullStr |
Fish Oil Enzymatic Esterification for Acidity Reduction |
title_full_unstemmed |
Fish Oil Enzymatic Esterification for Acidity Reduction |
title_sort |
Fish Oil Enzymatic Esterification for Acidity Reduction |
author |
Mata, Teresa M. |
author_facet |
Mata, Teresa M. Correia, Daniela Andrade, Soraia Casal, Susana Ferreira, Isabel M. P. L. V. O. Matos, Elisabete Martins, António A. Caetano, Nídia |
author_role |
author |
author2 |
Correia, Daniela Andrade, Soraia Casal, Susana Ferreira, Isabel M. P. L. V. O. Matos, Elisabete Martins, António A. Caetano, Nídia |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Politécnico do Porto |
dc.contributor.author.fl_str_mv |
Mata, Teresa M. Correia, Daniela Andrade, Soraia Casal, Susana Ferreira, Isabel M. P. L. V. O. Matos, Elisabete Martins, António A. Caetano, Nídia |
dc.subject.por.fl_str_mv |
Acidity reduction Enzymatic esterification Fish oil Free fatty acids Lipozyme TL 100L |
topic |
Acidity reduction Enzymatic esterification Fish oil Free fatty acids Lipozyme TL 100L |
description |
The reduction of the fish oil acidity is a significant problem in the rendering industry, as the oil’s range of applications and market value strongly depend on this parameter. In particular, the lower the acidity, the larger the oil’s market value. This work aims to study the potential of enzymatic esterification for reducing the fish oil acidity, by converting the free fatty acids into esters. Thus, four commercial lipases were used and a parametric study was performed to identify the best operating conditions, varying the reaction temperature, enzyme/oil mass ratio and alcohol/FFA mass ratio. All experiments were performed in duplicate with a very good reproducibility of results. Results showed that Lipozyme TL 100L contributed to greater acidity reduction (75% from an initial acid value of 10–14 mg KOH/g oil) for esterification at 40 °C, using ethanol 96% v/v, enzyme/oil and alcohol/FFA mass ratios of 0.01 and 3.24 w/w, respectively, reaching 3.13 mg KOH/g oil of final acid value or 1.57% FFA content. The reaction kinetics were also studied and it was found that a second order rate law as a function of the alcohol and oil concentrations is more adequate, with 35.44 kJ/mol of activation energy and 1.94 × 103 L mol− 1 min− 1 of pre-exponential factor. In conclusion, this work shows that the enzymatic esterification to reduce the fish oil acidity is technically feasible, increasing its market value. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020 2020-01-01T00:00:00Z 2100-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.22/17913 |
url |
http://hdl.handle.net/10400.22/17913 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1007/s12649-018-0357-z |
dc.rights.driver.fl_str_mv |
metadata only access info:eu-repo/semantics/openAccess |
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metadata only access |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799131465494036480 |