Newly developed microsatellite markers for the pan-European duck mussel, Anodonta anatina: Revisiting the main mitochondrial lineages

Detalhes bibliográficos
Autor(a) principal: Lopes-Lima, Manuel
Data de Publicação: 2016
Outros Autores: Sousa, Ronaldo, Teixeira, Amílcar, Varandas, Simone, Riccardi, Nicoletta, Aldridge, David C., Froufe, Elsa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10198/15336
Resumo: Freshwater mussels of the family Unionidae are one of the most threatened groups worldwide and have suffered severe decline over recent decades. Although the freshwater duck mussel, Anodonta anatina (Linnaeus, 1758), is still widespread, this species has shown evidence of recent declines and is already protected in some European countries. Informed conservation efforts must take into account patterns in genetic diversity and phylogeography. In the present study, 20 newly developed polymorphic loci were described and tested in seven populations of A. anatina, belonging to three previously detected divergent mtDNA lineages. The genetic diversity patterns, within and among A. anatina populations, were evaluated to test their congruence with those lineages. A high genetic differentiation (F ST ) was found among all populations, with the exception of those in Central Europe (Germany) and the UK, which were not strongly structured. The present study confirms the division of the species into three evolutionarily significant units corresponding to the three previously detected mtDNA lineages, which should be managed independently. Furthermore, owing to the high differentiation among southern European populations, the establishment of distinct management units for the Guadalquivir, Guadiana and Douro populations in the Iberian Peninsula is also proposed.
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spelling Newly developed microsatellite markers for the pan-European duck mussel, Anodonta anatina: Revisiting the main mitochondrial lineagesAnodonta anatinaAnodontiniConservation geneticsGenetic diversityMicrosatellitesUnionidaeFreshwater mussels of the family Unionidae are one of the most threatened groups worldwide and have suffered severe decline over recent decades. Although the freshwater duck mussel, Anodonta anatina (Linnaeus, 1758), is still widespread, this species has shown evidence of recent declines and is already protected in some European countries. Informed conservation efforts must take into account patterns in genetic diversity and phylogeography. In the present study, 20 newly developed polymorphic loci were described and tested in seven populations of A. anatina, belonging to three previously detected divergent mtDNA lineages. The genetic diversity patterns, within and among A. anatina populations, were evaluated to test their congruence with those lineages. A high genetic differentiation (F ST ) was found among all populations, with the exception of those in Central Europe (Germany) and the UK, which were not strongly structured. The present study confirms the division of the species into three evolutionarily significant units corresponding to the three previously detected mtDNA lineages, which should be managed independently. Furthermore, owing to the high differentiation among southern European populations, the establishment of distinct management units for the Guadalquivir, Guadiana and Douro populations in the Iberian Peninsula is also proposed.We would like to thank our collaborators who have kindly provided us with tissue samples: Juergen Geist, Katharina Stoeckl, and Irene Guarneri. Financial support was provided by the Portuguese Foundation for Science and Technology (FCT) project PTDC/AAC-AMB/117688/2010. We would like to thank Susana Lopes for help with fragment analysis and acknowledge the assistance of staff at Genoscreen for generation of 454 sequence reads and DNA sequencing. Official capture and sampling licences were issued by Junta de Castilla y León (Consejería de Fomento y Medio Ambiente EP/CYL/331/2012, de 17 de Abril de 2012); Junta de Andalucia (Consejería de Medio Ambiente- SGYB/FOA/AFR/SFS, de 17 de Julio de 2012); and Gobierno de Aragón (Dirección General de Conservación del Medio Natural- 136.071/2012 de 12 de Julio de 2012)Biblioteca Digital do IPBLopes-Lima, ManuelSousa, RonaldoTeixeira, AmílcarVarandas, SimoneRiccardi, NicolettaAldridge, David C.Froufe, Elsa2018-01-25T10:00:00Z20162016-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10198/15336engLopes-Lima, Manuel; Sousa, Ronaldo; Teixeira, Amílcar; Varandas, Simone; Riccardi, Nicoletta; Aldridge, David C.; Froufe, Elsa (2016). Newly developed microsatellite markers for the pan-European duck mussel, Anodonta anatina: Revisiting the main mitochondrial lineages. Aquatic Conservation: Marine and Freshwater Ecosystems. ISSN 1052-7613. 26, p. 307-3181052-761310.1002/aqc.2575info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-21T10:35:52Zoai:bibliotecadigital.ipb.pt:10198/15336Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T23:04:57.309629Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Newly developed microsatellite markers for the pan-European duck mussel, Anodonta anatina: Revisiting the main mitochondrial lineages
title Newly developed microsatellite markers for the pan-European duck mussel, Anodonta anatina: Revisiting the main mitochondrial lineages
spellingShingle Newly developed microsatellite markers for the pan-European duck mussel, Anodonta anatina: Revisiting the main mitochondrial lineages
Lopes-Lima, Manuel
Anodonta anatina
Anodontini
Conservation genetics
Genetic diversity
Microsatellites
Unionidae
title_short Newly developed microsatellite markers for the pan-European duck mussel, Anodonta anatina: Revisiting the main mitochondrial lineages
title_full Newly developed microsatellite markers for the pan-European duck mussel, Anodonta anatina: Revisiting the main mitochondrial lineages
title_fullStr Newly developed microsatellite markers for the pan-European duck mussel, Anodonta anatina: Revisiting the main mitochondrial lineages
title_full_unstemmed Newly developed microsatellite markers for the pan-European duck mussel, Anodonta anatina: Revisiting the main mitochondrial lineages
title_sort Newly developed microsatellite markers for the pan-European duck mussel, Anodonta anatina: Revisiting the main mitochondrial lineages
author Lopes-Lima, Manuel
author_facet Lopes-Lima, Manuel
Sousa, Ronaldo
Teixeira, Amílcar
Varandas, Simone
Riccardi, Nicoletta
Aldridge, David C.
Froufe, Elsa
author_role author
author2 Sousa, Ronaldo
Teixeira, Amílcar
Varandas, Simone
Riccardi, Nicoletta
Aldridge, David C.
Froufe, Elsa
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Biblioteca Digital do IPB
dc.contributor.author.fl_str_mv Lopes-Lima, Manuel
Sousa, Ronaldo
Teixeira, Amílcar
Varandas, Simone
Riccardi, Nicoletta
Aldridge, David C.
Froufe, Elsa
dc.subject.por.fl_str_mv Anodonta anatina
Anodontini
Conservation genetics
Genetic diversity
Microsatellites
Unionidae
topic Anodonta anatina
Anodontini
Conservation genetics
Genetic diversity
Microsatellites
Unionidae
description Freshwater mussels of the family Unionidae are one of the most threatened groups worldwide and have suffered severe decline over recent decades. Although the freshwater duck mussel, Anodonta anatina (Linnaeus, 1758), is still widespread, this species has shown evidence of recent declines and is already protected in some European countries. Informed conservation efforts must take into account patterns in genetic diversity and phylogeography. In the present study, 20 newly developed polymorphic loci were described and tested in seven populations of A. anatina, belonging to three previously detected divergent mtDNA lineages. The genetic diversity patterns, within and among A. anatina populations, were evaluated to test their congruence with those lineages. A high genetic differentiation (F ST ) was found among all populations, with the exception of those in Central Europe (Germany) and the UK, which were not strongly structured. The present study confirms the division of the species into three evolutionarily significant units corresponding to the three previously detected mtDNA lineages, which should be managed independently. Furthermore, owing to the high differentiation among southern European populations, the establishment of distinct management units for the Guadalquivir, Guadiana and Douro populations in the Iberian Peninsula is also proposed.
publishDate 2016
dc.date.none.fl_str_mv 2016
2016-01-01T00:00:00Z
2018-01-25T10:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10198/15336
url http://hdl.handle.net/10198/15336
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Lopes-Lima, Manuel; Sousa, Ronaldo; Teixeira, Amílcar; Varandas, Simone; Riccardi, Nicoletta; Aldridge, David C.; Froufe, Elsa (2016). Newly developed microsatellite markers for the pan-European duck mussel, Anodonta anatina: Revisiting the main mitochondrial lineages. Aquatic Conservation: Marine and Freshwater Ecosystems. ISSN 1052-7613. 26, p. 307-318
1052-7613
10.1002/aqc.2575
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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